Possible Involvement of Brain Tumour Stem Cells in the Emergence of a Fast-Growing Malignant Meningioma after Surgical Resection and Radiotherapy of High-Grade Astrocytoma: Case Report and Preliminary Laboratory Investigation

Shen, Fang; Chen, G; Shen, Gang; Liu, W; Zhou, Jia

doi:10.1177/147323000903700129

Cited by 11 publications

(11 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also used meningioma as additional positive control and comparison (Fig 2). Consistent with the literature,16,17 our data showed that meningioma was positive for CD133 and SDF-1 signals. Hemangioblastoma and meningioma appeared to be roughly similar to AVM tissues in the number of EPCs.…”

Section: Resultssupporting

confidence: 92%

Evidence of Endothelial Progenitor Cells in the Human Brain and Spinal Cord Arteriovenous Malformations

et al. 2010

View full text Add to dashboard Cite

Objective-Brain and spinal cord arteriovenous malformations (AVMs) are characterized by aberrant angiogenesis and vascular remodeling. Endothelial progenitor cells (EPCs) can be recruited by stromal cell-derived factor-1 (SDF-1), and participate in vascular remodeling in both physiological and pathological settings. We hypothesized that there was increased EPC levels in the brain and spinal cord AVM nidus.Address correspondence to: William L. Young, MD, University of California, San Francisco, Department of Anesthesia and Perioperative Care, 1001 Potrero Avenue, Room San Francisco, CA 94110, ccr@anesthesia.ucsf.edu. Financial DisclosuresThe authors have no personal, financial or institutional interest in any of the drugs, materials, or devices described in this article. AuthorshipThere were many collaborators in this interdisciplinary project. In addition to reviewing the final manuscript, the authors' participation is noted below.• Peng Gao, MD, PhD, drafted the manuscript, performed the laboratory assays in tissue, and participated in the study design and analysis.• Yongmei Chen, MD, PhD, performed the laboratory assays in tissue and participated in the study design and analysis.• Michael T. Lawton, MD, was responsible for the harvest and selection of surgical specimens, and participated in the histopathological screening and study design.• Nicholas M. Barbaro, MD, was responsible for harvest and selection of surgical specimens from epilepsy patients, and participated in the study design.• Guo-Yuan Yang, MD, PhD, participated in the study design.• Hua Su, MD, participated in the study design.• Feng Ling, MD, provided the human spinal cord AVM tissue, and participated in the study design.• William L. Young, MD, was involved in all aspects of the study.• UCSF BAVM Study Project members participated in other aspects of the work and are acknowledged. NIH Public Access Author ManuscriptNeurosurgery. Author manuscript; available in PMC 2011 October 1. Methods-Microsurgical specimens without endovascular embolization and radiosurgery from the brain (n=12) and spinal cord (n=5) AVMs were examined. Hemangioblastoma, meningioma, cerebral cortex obtained from epilepsy surgery, and the basilar artery (BA) from the autopsy were chosen for control comparisons. EPCs were identified as cells that were double-positive for the stem cell marker CD133 and the endothelial cell marker VEGFR-2 (vascular endothelial growth factor receptor-2 or KDR). In addition, SDF-1 was characterized by immunohistochemistry.Results-Both brain and spinal AVM tissues displayed more CD133, SDF-1, and CD68-positive signals than epilepsy and basilar artery control tissues. The level of EPCs was increased in the brain and spinal cord AVM nidus, mainly at the edge of the vessel wall. The expression of SDF-1 was colocalized with CD31-positive and α-smooth muscle cells, and was predominantly found within the vessel wall.Conclusion-Our data demonstrate that EPCs are present in the nidus of the brain and spinal cord AVMs, which may mediate pathologi...

show abstract

Section: Resultssupporting

confidence: 92%

Evidence of Endothelial Progenitor Cells in the Human Brain and Spinal Cord Arteriovenous Malformations

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Tumor stem cells are proposed to be the cellular origin of cancer including glioblastoma (GB) ( 3 ) and leukemia ( 4 ), are increasingly thought to be the origin of benign entities such as Dupuytren's disease ( 5 ), infantile hemangioma ( 6 ) and MG ( 1 , 7 – 9 ). Cultured MG cells form tumor spheres ( 8 ), demonstrate self-renewal, and express embryonic stem cell (ESC) associated markers including SOX2, nestin ( 1 ) and KLF4 ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

“…Recent reports have documented the presence of cells with stem cell phenotype within MG, although their exact location has not been clearly defined ( 1 , 7 , 8 , 10 , 11 ). Stem cells are characterized by their expression of certain ESC-associated markers including OCT4, SOX2, NANOG, KLF4 and c-MYC.…”

Section: Introductionmentioning

confidence: 99%

Expression of Embryonic Stem Cell Markers on the Microvessels of WHO Grade I Meningioma

et al. 2018

View full text Add to dashboard Cite

Aim: The presence of cells within meningioma (MG) that express embryonic stem cell (ESC) markers has been previously reported. However, the precise location of these cells has yet to be determined.Methods: 3,3-Diaminobenzidine (DAB) immunohistochemical (IHC) staining was performed on 11 WHO grade I MG tissue samples for the expression of the ESC markers OCT4, NANOG, SOX2, KLF4 and c-MYC. Immunofluorescence (IF) IHC staining was performed to investigate the localization of each of these ESC markers. NanoString and colorimetric in situ hybridization (CISH) mRNA expression analyses were performed on six snap-frozen MG tissue samples to confirm transcriptional activation of these proteins, respectively.Results: DAB IHC staining demonstrated expression of OCT4, NANOG, SOX2, KLF4, and c-MYC within all 11 MG tissue samples. IF IHC staining demonstrated the expression of the ESC markers OCT4, NANOG, SOX2, KLF4, and c-MYC on both the endothelial and pericyte layers of the microvessels. NanoString and CISH mRNA analyses confirmed transcription activation of these ESC markers.Conclusion: This novel finding of the expression of all aforementioned ESC markers in WHO grade I MG infers the presence of a putative stem cells population which may give rise to MG.

show abstract

“…The therapeutic options for the treatment of patients with astrocytoma are limited to three fundamental modalities: surgical resection, chemotherapy, and radiation therapy [4–6]. Cellular adoptive immunotherapy with specific CTLs has been recently used to treat malignant tumors [7–9].…”

Section: Introductionmentioning

confidence: 99%

Identification of new HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from neuritin

et al. 2013

View full text Add to dashboard Cite

Identification of cytotoxic T lymphocyte (CTL) epitopes from additional tumor antigens is essential for the development of specific immunotherapy of malignant tumors. Neuritin, a recently discovered antigen overexpressed in astrocytoma, is considered to be a promising target for biological therapy. In the present study, we predicted and identified HLA-A2-restricted CTL epitopes from neuritin by using the following four-step procedure: (1) computer-based epitope prediction from the amino acid sequence of neuritin; (2) peptide-binding assay to determine the affinity of the predicted peptide with HLA-A2.1 molecule; (3) stimulation of primary T cell response against the predicted peptides in vitro; and (4) testing of the induced CTLs toward target cells expressing neuritin and HLA-A2.1. The results demonstrated that effectors induced by peptides of neuritin containing residues 13–21, 121–129 and 4–12 could specifically-secrete interferon-γ and lyse target cells. Our results indicate that these peptides are new HLA-A2.1-restricted CTL epitopes, and may serve as valuable tools for astrocytoma immunotherapy.

show abstract

Possible Involvement of Brain Tumour Stem Cells in the Emergence of a Fast-Growing Malignant Meningioma after Surgical Resection and Radiotherapy of High-Grade Astrocytoma: Case Report and Preliminary Laboratory Investigation

Cited by 11 publications

References 34 publications

Evidence of Endothelial Progenitor Cells in the Human Brain and Spinal Cord Arteriovenous Malformations

Evidence of Endothelial Progenitor Cells in the Human Brain and Spinal Cord Arteriovenous Malformations

Expression of Embryonic Stem Cell Markers on the Microvessels of WHO Grade I Meningioma

Identification of new HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from neuritin

Contact Info

Product

Resources

About