Possible involvement of 4‐hydroxynonenal in splenocyte regulated liver regeneration

Čipak, Ana; Borović, Suzana; Ščukaneć-Špoljar, Mira; Kirac, Iva; Žarković, Neven

doi:10.1002/biof.5520240126

Cited by 6 publications

(8 citation statements)

References 31 publications

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“…This resembles our previous findings of the death of partially heptectomized mice bearing melanoma B16 or fibrosarcoma in the hind limb [28,29,31]. Partial hepatectomy is known to induce systemic inflammatory response [3]. In the tumor-bearing mice decreased tumor growth rates associated with necrosis of the tumors was observed after partial hepatectomy, but the reduction of the tumor mass was not associated with the extension of survival.…”

Section: Resultssupporting

confidence: 89%

Oxidative burst and anticancer activities of rat neutrophils

et al. 2005

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It is assumed that oxidative damage caused by reactive oxygen species (ROS) from activated neutrophil granulocytes may contribute to pathology of tumors. ROS are crucial in neutrophil-mediated tumor cell lysis. The present study is focused on the oxidative burst and antitumorous activities of neutrophils when challenged with Walker carcinoma W256. Survival and tumor growth dynamics were monitored in vivo, while tumor cell proliferation when mixed with neutrophils was studied in vitro together with the generation/release of neutrophil respiratory burst products, primarily 1O2. Neutrophils were collected upon Sephadex injection. The survival of Sephadex injected animals was slightly improved, while their tumors grew less than in controls. The presence of tumor cells in vitro activated neutrophils to produce singlet oxygen similar to phorbol ester. Neutrophils from Sephadex-bearing animals diminished tumor cell proliferation in vitro (measured by 3H-TdR incorporation), while neutrophils from Sephadex and the tumor-bearing animals did not show such activity in vitro. Our results confirm that in the case of rapidly growing tumors such as murine W256 carcinoma neutrophils have antitumorous effects in the early phase of tumor development.

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Section: Resultssupporting

confidence: 89%

Oxidative burst and anticancer activities of rat neutrophils

et al. 2005

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“…In contrast, when hepatocytes were exposed to HNE and cultivated with spleen cells, there was a significant decrease (P < 0.001) of apoptosis and necrosis compared to hepatocytes exposed to HNE but cultured without spleen cells. These results show similarity to the results for LDP/OC cells published previously (Cipak et al, 2005).…”

Section: Effects Of Spleen Cells On Apoptosis and Necrosis In Primarysupporting

confidence: 92%

“…LDP/OC cells. A liver-derived progenitor/oval-like cell line (referred to as LDP/OC) was isolated as described previously (Cipak et al, 2005). Briefly, liver was minced by pushing through a 10 ml syringe and put in a beaker on ice for 5 min to allow large particles to settle.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous work we have shown that a cell line derived from liver damaged by HNE (Cipak et al, 2005), recovers in the presence of spleen cells, which migrate as inflammatory cells to the damaged liver and affect its regeneration. In this report, the aim was to characterize a cell line derived from liver and to evaluate the effects of exposure to HNE and of spleen cells on the cells of liver origin, which should eventually help to clarify liver regeneration mechanisms.…”

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confidence: 99%

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Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells.

Čipak

Borović²,

Jaganjac³

et al. 2010

Acta Biochim Pol

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Liver is a unique mammalian organ with a great capacity of regeneration related to its function. After surgical resection or injury, hepatic cells, especially hepatocytes, can proliferate rapidly to repair the damage and to regenerate the structure without affecting the function of the liver. Loss of catalase activity during regeneration indicates that oxidative stress is present in the liver not only in pathological conditions but also as a 'physiological' factor during regeneration. As we have shown in our previous work, liver stem cell-like cells treated with 4-hydroxynonenal (HNE), a cytotoxic and growth regulating lipid peroxidation product, recover in the presence of spleen cells. In the current study we characterized this novel cell line as liver-derived progenitor/oval-like cells, (LDP/OCs), i.e. functional liver stem-like cells. We showed that LDP/OC were OV6 positive, with abundant glycogen content in the cytoplasm and expressed alpha-fetoprotein, albumin, biliverdin reductase and gamma-glutamyl transferase. Also, we compared their growth in vitro with the growth of cultured primary hepatocytes stressed with HNE and co-cultured with autologous spleen cells. The influence of spleen cells on HNE-treated primary hepatocytes and on LDP/OCs showed that spleen cells support in a similar manner the recovery of both types of liver cells indicating their important role in regeneration. Hence, LDP/OC cells may provide a valuable tool to study cell interactions and the role on HNE in liver regeneration.

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“…The lipid peroxidation products are liberated in the circulation as the membrane lipids are oxidized during cell lysis. These products are also a source of cytotoxicity and are suggested to invoke the inflammatory response in the body [24,25]. Hence, an increased TBARS level may reflect the largescale destruction of hepatocytes that may further aggravate the inflammation and cell death.…”

Section: Discussionmentioning

confidence: 99%