Possible cryptic speciationwithin the sea anemone Actinia equina complex detected by AFLP markers

Douek, Jacob; Barki, Y.; Gateño, D.; Rinkevich, Baruch

doi:10.1046/j.1096-3642.2002.00034.x

Cited by 34 publications

(16 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are consistent with AFLP data reported for other European invertebrates such as the soft coral Parerythropodium fulvum fulvum (Barki et al . 2000), the sea anemone Actinia equina (Douek et al . 2002) and the dung beetle of the genus Trypocopris (Carisio et al .…”

Section: Discussionmentioning

confidence: 99%

Gene‐flow patterns in Atlantic and Mediterranean populations of the Lusitanian sea star Asterina gibbosa

2005

View full text Add to dashboard Cite

In this study, the population structure of the Lusitanian sea star Asterina gibbosa was assessed using amplified fragment length polymorphism (AFLP). One hundred and twenty-two AFLP loci were analysed in 159 individuals from eight populations from across the species' range and revealed high levels of genetic diversity, with all individuals but two harbouring a unique banding pattern. As reported for other marine invertebrates, we found high levels of genetic differentiation between the Atlantic and Mediterranean basins, suggesting that the Strait of Gibraltar represents a major barrier to dispersal for this sea star. Our assignment studies suggest that, in the Atlantic, a measurable degree of gene flow occurs between populations, which could result in the isolation-by-distance pattern of differentiation observed in this basin. In contrast, no evidence of contemporary gene flow was found in the Mediterranean, suggesting contrasting patterns of dispersal of Asterina gibbosa in the Atlantic and Mediterranean basins.

show abstract

Section: Discussionmentioning

confidence: 99%

Gene‐flow patterns in Atlantic and Mediterranean populations of the Lusitanian sea star Asterina gibbosa

2005

View full text Add to dashboard Cite

show abstract

“…Genomic DNA was extracted according to Graham (1978), with the following modifications (Douek et al, 2002(Douek et al, , 2010Israel et al, 2010): the samples were centrifuged for 5 min at 14,000 rpm; the aqueous upper phase was transferred to a new tube, further mixed with 240 µl of chloroform-isoamyl alcohol (24:1, v/v) and centrifuged for 5min at 14,000rpm. The upper phase was then collected once more and the DNA was precipitated overnight at -20°C, with 500µl of 100% cold ethanol.…”

Section: Dna Extraction Amplification Sequencingmentioning

confidence: 99%

Initiating DNA barcoding of Eastern Mediterranean deep-sea biota

et al. 2018

View full text Add to dashboard Cite

Preliminary results of DNA barcoding survey of deep-sea mega-faunal biota are presented, collected by trawl and gillnet off the Israeli coast (SE Mediterranean, depths 700 to 1500 meters) during 2012-2013. 846 organisms were identified to 37 species, mainly fish and decapod crustaceans. The most abundant species were the blackmouth catshark Galeus melastomus, the cosmopolitan decapod Polycheles typhlops and the bivalve Abra longicallus. Two species were sampled for the first time from the southern Levant- the long armed chiroteuthid squid Chiroteuthis veranyi and the common mora, Mora moro. Four of the 18 fish species and two of the 10 crustacean species were abundant, representing 78% and 61%, respectively, of the organisms collected. Most other species are represented by fewer than 10 individuals. PCR products for the cytochrome c oxidase sub unit I (COI) gene for the 37 species were successfully sequenced. The identified and vouchered individuals are stored at the Steinhardt Museum of Natural History (Tel Aviv University, Israel) and their COI sequences were uploaded into the BoLD universal data center as part of the national marine barcoding project. The COI sequences of Acanthephyra eximia, Gryphus vitreus, Galeodea echinophora, Mesothuria intestinalis and Astropecten irregularis, constitute first records of these species in BoLD. When compared to the COI sequences in BoLD, the present results reveal some inconsistency in species identification, an outcome that should be taken into consideration primarily once the taxonomical verifications of collected taxa are elusive. This study is the first step in DNA barcoding of the Levant’s little-known benthic deep-sea fauna.

show abstract

“…We used analysis of amplified fragment length polymorphisms (AFLPs) to evaluate whether G. laevigata may be a complex of cryptic species. These molecular markers allow assessment of a large number of DNA fragment polymorphisms throughout the genome in a large number of individuals and are becoming an increasingly important tool for identifying early stages of divergence in a wide range of taxa (28)(29)(30)(31).…”

mentioning

confidence: 99%

Cryptic species within the cosmopolitan desiccation-tolerant moss Grimmia laevigata

Fernandez

Shevock

Glazer

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The common cushion moss Grimmia laevigata (Bridel) Bridel grows on bare rock in a broad range of environments on every continent except Antarctica. As such, it must harbor adaptations to a remarkably broad set of environmental stresses, the extremes of which can include very high temperatures, prolonged nearly complete desiccation, and high ultraviolet B (UVB) exposure. Yet, like many mosses, G. laevigata shows very little morphological variability across its cosmopolitan range. This presents an evolutionary puzzle, the solution to which lies in understanding the phylogeographic structure of this morphologically simple organism. Here we report the results of an analysis of amplified fragment length polymorphisms (AFLPs) in G. laevigata, focusing on individuals from the California Floristic Province. We found evidence that populations within California constitute two distinct geographically overlapping cryptic species. Each clade harbors multiple private alleles, indicating they have been genetically isolated for some time. We suggest that the existence of cryptic species within G. laevigata, in combination with its life history, growth habits, and extreme desiccation tolerance, makes this moss an ideal research tool and a candidate for a biological indicator of climate change and pollution.amplified fragment length polymorphisms ͉ bioindication ͉ California Floristic Province ͉ Grimmiaceae ͉ phylogeography

show abstract

Possible cryptic speciationwithin the sea anemone Actinia equina complex detected by AFLP markers

Cited by 34 publications

References 24 publications

Gene‐flow patterns in Atlantic and Mediterranean populations of the Lusitanian sea star Asterina gibbosa

Gene‐flow patterns in Atlantic and Mediterranean populations of the Lusitanian sea star Asterina gibbosa

Initiating DNA barcoding of Eastern Mediterranean deep-sea biota

Cryptic species within the cosmopolitan desiccation-tolerant moss Grimmia laevigata

Contact Info

Product

Resources

About