Possible assignment of the glyoxalase I (GLO) gene to chromosome 6 using man-mouse somatic cell hybrids

Abstract

A correlation between the expression or absence of human glyoxalase I and chromosome 6 (as well its markers ME1, IPO-B, and PGM3) was observed in man-mouse somatic cell hybrids. This segregation pattern indicates that the GLO gene is situated on chromosome 6.

Regional localization of loci on chromosome 14 using somatic cell hybrids

“…Rag-G03-1. obtained from the original somatic cell hybrid line RagGordon (Bender andSiniscalco. 1976), is a hybrid derived from fusion of mouse Rag cells with human fibroblasts carry ing the balanced translocation t(X;14)(pl !:q32).…”

Glyoxalase I ‘Null’ Allele in the Polish Population

“…Hemolysates were subjected to starch electro phoresis in 0.1 M Tris-maleate buffer, pH 7.4 (method of Kiihnl et al (1977) modified by Byrdy and Trembaczowska [1980]); the gel was stained ac cording to Bender and Grzeschik [1976] with Raczek's modification [1987], Glyoxalase activity was determined according to Racker [1951] and Sparkes |letter commun., 1986Sparkes |letter commun., -1988, The hemoglobin concentration was mea sured in Drabkin's solution.…”

The elastin gene is disrupted in a family with a balanced translocation t(7;16)(q11.23;q13) associated with a variable expression of the Williams-Beuren syndrome

“…NA10658), was fused with Polyethlenglycol (PEG) in equal proportion with human lymphoblastoid cells from the affected individual III/7 as described earlier. 23 Individual hybrid clones were analysed by PCR using the chromosome 7 markers D7S544E and D7S557E for the presence of chromosome 7 or its derivatives. Clones containing either the marker from the telomeric end of 7q or from 7p, respectively, were selected for further analysis.…”