Possible Ameliorating Role of Ascorbic Acid on Intestinal Changes Induced by Acrylamide in Adult Female Albino Rats and Their Offsprings

Abstract

Background: Acrylamide (ACR) is a naturally occurring, widely used compound. Ingestion of large amounts of ACR underlies several health concerns and teratogenicity. Ascorbic acid (vitamin C) is a strong reducing agent greatly used to clean free radicals. This study investigated the morphometric, histological, immunohistochemical and biochemical disturbances induced by acrylamide (10 mg/kg/day) via gavage in the intestine of rat mothers and their offsprings. As well as, the protective role of ascorbic acid (100 mg/kg/day) via gavage. Materials and Methods: Forty adult pregnant female rats were divided into four groups; control, ascorbic acid, acrylamide and acrylamide+ascorbic acid. 10 randomly chosen offsprings of each group after weaning were also used. Histomorphometric analysis of intestinal wall and biochemical analysis of intestinal enzymes, oxidant antioxidant markers and some genes expression were performed. Results: In both dams and offsprings, ACR resulted in mucosal hyperplasia with evident inflammatory infiltration in the villi. In addition, goblet cells and KI67 +ve cell numbers decreased in the dams however increased in offsprings. ACR decreased citrate synthase, glutathione and catalase levels in dams and increased β-glucuronidase and malonaldehyde levels in dams. In offsprings, level of alkaline phosphatase was reduced and β-glucuronidase was elevated. Glutathione peroxidase and glutathione reductase mRNA expression was increased significantly with ACR ingestion. Ascorbic acid supplementation conserved the control status in the majority of conditions. Conclusion: Acrylamide consumption during pregnancy and lactation is risky because of the induction of intestinal mucosal hyperplasia in rat offsprings. Ascorbic acid supplementation could reduce the harmful effects induced by ACR.

Bisphenol A enhances apoptosis, fibrosis, and biochemical fluctuations in the liver of adult male rats with possible regression after recovery

“…All kits were supplied by Qiagen (Valencia, CA). All samples were assessed in triplicate to confirm the reproducibility and accuracy of the obtained results (Aboregela et al, 2020; Kassab et al, 2020). The primers were designed according to (Al‐Rasheed et al, 2016), as shown in Table 1b.…”

The ameliorating role of sofosbuvir and daclatasvir on thioacetamide-induced kidney injury in adult albino rats

“…PBS kept kidney samples were homogenized in ice-cold 0.1 M Tris-HCl buffer at pH 7.4. Centrifugation of the homogenate at 3,000 rpm for 15 minutes at 4°C was done and the resultant supernatant was isolated for detection of Malondialdehyde (MDA) and Reduced glutathione (GSH) levels [ 35 ]. The pellets were extracted and stored at −20°C [ 36 ].…”

Histomorfometri Duodenum Mencit (Mus musculus) yang Diinfeksi Telur Infektif Hymenolepis nana dan diberi Ekstrak Daun Kelor (Moringa oleifera Lam.)

“…Tinggi vili normal mencit adalah 333,25±45,25 μm (Hidayat et al, 2021), dan tinggi vili usus halus mencit adalah 288,95±7,73 μm (Zou & Zheng, 2013). Tinggi vili normal duodenum mencit adalah 356,58±21,21 μm (Aboregela et al, 2020). Tinggi vili pada kelompok K3 menunjukkan bahwa ekstrak daun kelor LD100 efektif mengurangi peradangan usus sehingga terjadi penyembuhan luka dan perbaikan struktur vili duodenum.…”

“…Penebalan lapisan mukosa duodenum dari ketiga kelompok perlakuan berada di atas kisaran normal tebal mukosa duodenum mencit. Tebal mukosa normal duodenum mencit berkisar antara 342,32-412,85 µm (Aboregela et al, 2020). Penebalan mukosa duodenum setiap kelompok perlakuan terjadi karena adanya peradangan akibat infeksi cacing cestoda.…”

“…Sedangkan kelompok K1 tidak berbeda nyata dengan K2 (Sig >0.05). Tebal lapisan sub-mukosa duodenum mencit kelompok K1 sebesar 16,67 ± 0,26 μm, kelompok K2 sebesar 16,50 ± 0,22 μm, dan kelompok K3 sebesar 13,81 ± 0,35 μm.Tebal sub-mukosa normal duodenum mencit berkisar antara 12,25±1,98 -260,37±21,78 µm (Aboregela et al, 2020). Berdasarkan hal tersebut, tebal lapisan sub-mukosa setiap kelompok perlakuan masuk ke dalam tebal sub-mukosa normal.…”