Positiver Kontrast zum Nachweis magnetisch markierter Zellen in der MRT – in-vitro Experimente / Positive contrast in the detection of magnetically labeled cells by MRI – in vitro experiments

Pintaske, Jörg; Martirosian, Petros; Claussen, Claus D.; Schick, Fritz

doi:10.1515/bmt.2005.040

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…Superparamagnetic iron oxide and ultra small super-paramagnetic iron oxide predominantly shortens the T2 and T2 * relaxation time ( 32 – 34 ). Using conventional MRI contrast agents, it is difficult to distinguish stem cells from air, post-operative bleeding and iron signals originating from red blood cells ( 35 , 36 ). These effects lead to distortion and misinterpretation of MRI tracing of stem cells.…”

Section: Discussionmentioning

confidence: 99%

Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats

Tang

et al. 2015

Molecular Medicine Reports

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Although studies have been undertaken on gadolinium labeling-based molecular imaging in magnetic resonance imaging (MRI), the use of non-ionic gadolinium in the tracking of stem cells remains uncommon. To investigate the efficiency in tracking of stem cells with non-ionic gadolinium as an MRI contrast agent, a rhodamine-conjugated fluorescent reagent was used to label bone marrow stromal cells (BMSCs) of neonatal rats in vitro, and MRI scanning was undertaken. The fluorescent-conjugated cell uptake reagents were able to deliver gadodiamide into BMSCs, and cell uptake was verified using flow cytometry. In addition, the labeled stem cells with paramagnetic contrast medium remained detectable by an MRI monitor for a minimum of 28 days. The present study suggested that this method can be applied efficiently and safely for the labeling and tracking of bone marrow stromal cells in neonatal rats.

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Section: Discussionmentioning

confidence: 99%

Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats

Tang

et al. 2015

Molecular Medicine Reports

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Gadolinium‐enhanced off‐resonance contrast angiography

Edelman

Storey

Dunkle

et al. 2007

Magnetic Resonance in Med

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We describe a novel physical basis and methodology for gadolinium (Gd)-enhanced MRA, which we call "off-resonance contrast angiography" (ORCA). Unlike standard contrast-enhanced (CE) MR angiography (MRA), ORCA contrast depends not on T 1 but on Gd-induced shifts in intravascular resonance frequency due to the bulk magnetic susceptibility (BMS) effects of Gd. The method was tested at 3 Tesla in phantoms with a range of dilutions of Gd-DTPA and ultrasmall iron oxide contrast agent (CA). With the use of ORCA, complete background suppression was obtained without image subtraction. As a result, catheters filled with various Gd dilutions proved to be highly conspicuous in ORCA projection images. This feature may make ORCA particularly attractive for passive catheter tracking during MRguided endovascular procedures. Gd-induced intravascular frequency shifts were measured in human subjects and found to be in the expected range. ORCA was used to create angiograms of forearm veins that were comparable in quality to standard CE-MRA. In addition, ORCA images of the extracranial carotid bifurcation were successfully acquired during intravenous contrast administration. However, significant technical restrictions also exist, including a dependence on vessel orientation with respect to B 0 , and sensitivity to static field inhomogeneities. Key words: magnetic resonance angiography; contrast agents; bulk magnetic susceptibility; off-resonance; chemical shift First-pass contrast-enhanced (CE) MR angiography (MRA) is a widely used, robust method for depicting vascular anatomy (1-8). The method is predicated on the strong T 1 -dependent contrast between gadolinium (Gd)-enhanced blood vessels and background tissues.We describe a novel physical basis and methodology for Gd-enhanced MRA, which we call "off-resonance contrast angiography" (ORCA). Unlike standard CE-MRA, ORCA contrast depends not on T 1 but on Gd-induced shifts in intravascular resonance frequency due to the bulk magnetic susceptibility (BMS) of Gd. If the excitation frequency and bandwidth are properly chosen, one can excite selected concentrations of contrast agent (CA) without exciting fat or water spins. Moreover, complete background suppression can be obtained without image subtraction. We studied the feasibility and limitations of the ORCA methodology in studies of phantoms and human subjects. MATERIALS AND METHODSThis clinical study was approved by the hospital institutional review board. A total of 11 subjects (three males and eight females, 19 -38 years old) were imaged. Five subjects were studied for measurement of intravascular frequency shifts, and six were measured as a proof of concept for vascular imaging with ORCA.

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Quantification of direct current in electrically active implants using MRI methods

Wojtczyk

Graf

Martirosian

et al. 2011

Zeitschrift für Medizinische Physik

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Positiver Kontrast zum Nachweis magnetisch markierter Zellen in der MRT – in-vitro Experimente / Positive contrast in the detection of magnetically labeled cells by MRI – in vitro experiments

Cited by 4 publications

References 11 publications

Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats

Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats

Gadolinium‐enhanced off‐resonance contrast angiography

Quantification of direct current in electrically active implants using MRI methods

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