Positive and negative regulators for neuronal BC1 RNA transcription by RNA polymerase III are possible members of the RNA polymerase II transcription system

Kobayashi, Shunsuke; Kamo, Sakurako; Agui, Keisuke; Funakoshi, Tomoko; Anzai, Kentaro

doi:10.1016/s0169-328x(02)00695-2

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…The human counterpart of BC1 is BC200, a neural‐specific RNA that is also targeted to dendrites (Tiedge et al, 1993). Whereas BC1 is known to bind to Purα (Kobayashi et al, 2003), BC200 has not yet been examined in that regard. We sought to determine whether BC200 is bound by Purα and whether Purα can mediate the association of such non‐coding RNA molecules with dendritic mRNA molecules.…”

Section: Resultsmentioning

confidence: 99%

Role of Purα in targeting mRNA to sites of translation in hippocampal neuronal dendrites

Johnson

Kinoshita

Weinreb

et al. 2006

J of Neuroscience Research

115

143

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Using genetic inactivation in the mouse, PURA, encoding Pur alpha, is demonstrated to be essential for developmentally-timed dendrite formation in the cerebellum and hippocampus. Comparison of RNA species bound by Pur alpha prompts the hypothesis that Pur alpha functions with non-coding RNA in transport of certain mRNA molecules to sites of translation in dendrites. Pur alpha binds to human BC200 RNA, implicated in dendritic targeting, and this has homologies to 7SL RNA, implicated in compartmentalized translation. Results using hippocampal rat neurons in situ show that Pur alpha binds to BC1 RNA, implicated in dendritic targeting as a mouse counterpart of BC200, and to mRNA molecules translated in dendrites; Pur alpha is specifically located in dendrites, where it is colocalized with Map2, but not in axons, where it fails to colocalize with Ankyrin G. Pur alpha and Staufen are colocalized at dendritic sites of mRNA translation. Microtubule disruptors inhibit Pur alpha dendritic targeting and allow its mislocalization to axons. Using mouse brain, double-RNA immunoprecipitation places Pur alpha together with Staufen or FMRP on BC1 RNA and specific mRNA species in vivo. These results help define a mechanism by which Pur alpha targets specific mRNA molecules to sites of dendritic translation.

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Section: Resultsmentioning

confidence: 99%

Role of Purα in targeting mRNA to sites of translation in hippocampal neuronal dendrites

Johnson

Kinoshita

Weinreb

et al. 2006

J of Neuroscience Research

115

143

View full text Add to dashboard Cite

show abstract

YB-1 transcription in the postnatal brain is regulated by a bHLH transcription factor Math2 through an E-box sequence in the 5′-UTR of the gene

et al. 2009

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YB-1 controls gene expression at both the transcriptional and translational levels. In the brain, a high level of YB-1 is expressed from prior to the first week after birth. However, YB-1 expression substantially declines to the adult level thereafter. Here, we investigated the regulatory mechanism of YB-1 transcription in the postnatal brain. We found that an E-box sequence within the 5'-untranslated region (5'-UTR) of the gene was recognized by Math2 in the 5-day-old brain, whereas the DNA binding was reduced in the 4-week-old brain. In vitro transcription analysis and reporter gene assay revealed that Math2 was necessary for YB-1 transcription. Furthermore, the expression pattern of Math2 protein in the postnatal brain was correlated with that of YB-1 protein. Our results suggest that YB-1 transcription in the postnatal brain is regulated by Math2, which binds to an E-box in the 5'-UTR of the YB-1 gene.

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Application of the BC1 RNA gene promoter for short hairpin RNA expression in cultured neuronal cells

Kobayashi¹,

Higuchi²,

Anzai³

2005

Biochemical and Biophysical Research Communications

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Positive and negative regulators for neuronal BC1 RNA transcription by RNA polymerase III are possible members of the RNA polymerase II transcription system

Cited by 3 publications

References 22 publications

Role of Purα in targeting mRNA to sites of translation in hippocampal neuronal dendrites

Role of Purα in targeting mRNA to sites of translation in hippocampal neuronal dendrites

YB-1 transcription in the postnatal brain is regulated by a bHLH transcription factor Math2 through an E-box sequence in the 5′-UTR of the gene

Application of the BC1 RNA gene promoter for short hairpin RNA expression in cultured neuronal cells

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