Position-specific glucosylation of salicyl alcohol with an enzyme preparation from Gardenia jasminoides cultured cells

Partial Purification and Characterization of UDP-Glucose: Salicyl Alcohol Glucosyltransferase fromGardenia jasminoidesCell Cultures

“…glucosyltransferase showed the optimum pH of reaction at pH 9,0-9.5. This optimum pH is much higher than those of other plant glucosyltransferases so far reported (5). The enzyme was stored overnight at 4° C at a range of pH 3.0-12.0 and the remaining activity of the enzyme was measured at pH 9.0 (Fig.…”

“…In our previous studies (4)(5), however, we found that cell cultures of Gardenia jasminoides and Lithospermum erythrorhizon produce salicin as well as isosalicin from salicyl alcohol and we detected enzyme activity catalyzing the formation of salicin from salicyl alcohol and UDP-glucose in a cell-free preparation from G. jasminoides cultured cells (Scheme).…”

“…The values of slopes and vertical intercepts were replotted to give secondary plots, from which Km values for salicyl alcohol and UDP-glucose were calculated to be 0.11 and 0.031 mM, respectively. This Km value for UDP-glucose was much smaller than that obtained by using the crude enzyme (5). One of the possible reasons for this may be due to presence of the enzymes Introduction Oenanthe aquatica L. (Umbelliferae) is a herbaceous water plant endemic to the marshy areas of continental Europe.…”