1985
DOI: 10.1055/s-2007-969419
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Partial Purification and Characterization of UDP-Glucose: Salicyl Alcohol Glucosyltransferase fromGardenia jasminoidesCell Cultures

Abstract: A new enzyme, UDP-glucose : saucy! alcohol glucosyltransferase, was purified 110-fold from Gardenia jasminoides cell cultures. The enzyme catalyzes position-specific glucosylation of saucy! alcohol to form salicin using UDP-glucose as a glucose donor. The molecular weight of the enzyme as estimated by gel filtration is 51,000. Apparent Km values for saucy! alcohol and UDP-glucose are 0.11 and 0.03 1 mM, respectively. The enzyme shows a pH optimum between 9.0 and 9.5. Sulfhydryl inhibitors and some divalent cat… Show more

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Cited by 17 publications
(3 citation statements)
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“…It would appear that the biosynthetic pathways to cyanogenic glycosides such as dhurrin and the nitro-compound (67) are closely related (Scheme 23). A glucosyltransferase which catalyses the glucosylation of ( 67) to (68) in the presence of UDPglucose was also present in the osmotically stressed cell cultures, appearing in a time pattern similar to that for the production of (67).…”
Section: Cinnamic Acids and Estersmentioning
confidence: 89%
“…It would appear that the biosynthetic pathways to cyanogenic glycosides such as dhurrin and the nitro-compound (67) are closely related (Scheme 23). A glucosyltransferase which catalyses the glucosylation of ( 67) to (68) in the presence of UDPglucose was also present in the osmotically stressed cell cultures, appearing in a time pattern similar to that for the production of (67).…”
Section: Cinnamic Acids and Estersmentioning
confidence: 89%
“…Several groups showed, that simple phenols like salicylic acid, salicyl alcohol or salicylaldehyde .can be glucosylated very effectively (Mizukami et al 1983(Mizukami et al , 1985(Mizukami et al , 1986(Mizukami et al , 1987Pilgrim 1970;Petersen et al 1992;Tabata et al 1976Tabata et al , 1988Tanaka et al 1990). An interesting reaction in this context is the glucosylation of hydroquinone to arbutine.…”
Section: Introductionmentioning
confidence: 99%
“…Several groups showed, that simple phenols like salicylic acid, salicyl alcohol or salicylaldehyde .can be glucosylated very effectively (Mizukami et al 1983(Mizukami et al , 1985(Mizukami et al , 1986(Mizukami et al , 1987Pilgrim 1970;Petersen et al 1992;Tabata et al 1976Tabata et al , 1988Tanaka et al 1990). Several groups showed, that simple phenols like salicylic acid, salicyl alcohol or salicylaldehyde .can be glucosylated very effectively (Mizukami et al 1983(Mizukami et al , 1985(Mizukami et al , 1986(Mizukami et al , 1987Pilgrim 1970;Petersen et al 1992;Tabata et al 1976Tabata et al , 1988Tanaka et al 1990).…”
Section: Introductionmentioning
confidence: 99%