Portable oxidative stress sensor: Dynamic and non-invasive measurements of extracellular H 2 O 2 released by algae

Koman, Volodymyr B.; Santschi, Christian; Moos, Nadia Rachel Von; Martin, Olivier J. F.

doi:10.1016/j.bios.2014.12.044

Cited by 15 publications

(20 citation statements)

References 35 publications

(38 reference statements)

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“…8) [82]. An aqueous solution of cyt c was spotted onto a porous filter membrane using a microarray robot with a delivery volume of 5 nl of 4 mM cyt c solution.…”

Section: Portable Setup and Microfluidic Chipmentioning

confidence: 99%

“…The sensing element was placed at the bottom of the chamber prior to the measurements. Figure 9a shows the time evolution of φ in the static regime for different H 2 O 2 concentrations in PBS buffer solution [82]. Measurements performed in this configuration exhibit a signal enhancement due to multiscattering, on the order of 5.…”

Section: Portable Setup and Microfluidic Chipmentioning

confidence: 99%

“…The calibration curve resembles a sigmoidal shape when increasing H 2 O 2 concentration, which is typical for ligand binding assays and can be fitted using a 4-parameter logistic model [84]. For the given configuration with a porous membrane a LOD of 40 nM of H 2 O 2 was achieved [82]. Exposing the sensing element to reducing agents the cyt c alters from its ferric Fe III state to its ferrous Fe II state.…”

Section: Portable Setup and Microfluidic Chipmentioning

confidence: 99%

“…This has been shown by exposing the sensing spot to AA. Four consecutive oxidation/reduction cycles were carried out without lowering the performance of the sensor [82]. Furthermore, glucose and H 2 O 2 and lactate and H 2 O 2 were simultaneously measured adding glucose (GOx) and lactate oxidase (LOx), respectively, for the enzymatic conversion into H 2 O 2 [Eq.…”

Section: Portable Setup and Microfluidic Chipmentioning

confidence: 99%

“…Here ENM-induced H 2 O 2 excretion by cells exposed to ENMs was monitored with a recently developed optical biosensor in a portable setup (POSS; portable oxidative stress sensor) specifically designed for field experimentation [82]. In this way, POSS may contribute to the elucidation of ENM-specific pro-oxidant interactions with cells and thus help to narrow the gap between material innovation and sound risk assessment.…”

Section: Portable Setup and Microfluidic Chipmentioning

confidence: 99%

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Non-invasive continuous monitoring of pro-oxidant effects of engineered nanoparticles on aquatic microorganisms

et al. 2017

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Engineered nanomaterials (ENMs) are key drivers for the development of highly sophisticated new technologies. As all new attainments, the rapidly increasing used of ENMs raise concerns about their safety for the environment and humans. There is growing evidence showing that if engineered nanomaterials are released into the environment, there is a possibility that they could cause harm to aquatic microorganisms. Among the divers effects triggering their toxicity the ability of ENMs to generate reactive oxygen species (ROS) capable of oxidizing biomolecules is currently considered a central mechanism of toxicity. Therefore, development of sensitive tools for quantification of the ROS generation and oxidative stress are highly sought. After briefly introducing ENMs-induced ROS generation and oxidative stress in the aquatic microorganisms (AMOs), this overview paper focuses on a new optical biosensor allowing sensitive and dynamic measurements of H2O2 in real-time using multiscattering enhanced absorption spectroscopy. Its principle is based on sensitive absorption measurements of the heme protein cytochrome c whose absorption spectrum alters with the oxidation state of constituent ferrous FeII and ferric FeIII. For biological applications cytochrome c was embedded in porous random media resulting in an extended optical path length through multiple scattering of light, which lowers the limit of detection to a few nM of H2O2. The sensor was also integrated in a microfluidic system containing micro-valves and sieves enabling more complex experimental conditions. To demonstrate its performance, abiotic absorption measurements of low concentrations of dye molecules and 10 nm gold particles were carried out achieving limits of detection in the low nM range. Other biologically relevant reactive oxygen species can be measured at sub-μM concentrations, which was shown for glucose and lactate through enzymatic reactions producing H2O2. In ecotoxicological investigations H2O2 excreted by aquatic microorganisms exposed to various stressors were measured. Pro-oxidant effects of nano-TiO2 and nano-CuO towards green alga Chlamydomonas reinhardtii were explored in various exposure media and under different light illuminations. Dynamics of Cd2+ induced effects on photosynthetic activity, sensitisation and recovery of cells of C. reinhardtii was also studied.

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“…8) [82]. An aqueous solution of cyt c was spotted onto a porous filter membrane using a microarray robot with a delivery volume of 5 nl of 4 mM cyt c solution.…”

Section: Portable Setup and Microfluidic Chipmentioning

confidence: 99%

Section: Portable Setup and Microfluidic Chipmentioning

confidence: 99%

Section: Portable Setup and Microfluidic Chipmentioning

confidence: 99%

Section: Portable Setup and Microfluidic Chipmentioning

confidence: 99%

Section: Portable Setup and Microfluidic Chipmentioning

confidence: 99%

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Non-invasive continuous monitoring of pro-oxidant effects of engineered nanoparticles on aquatic microorganisms

et al. 2017

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Assessment of the oxidative potential of nanoparticles by the cytochrome c assay: assay improvement and development of a high-throughput method to predict the toxicity of nanoparticles

et al. 2016

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Oxidative stress has increasingly been demonstrated as playing a key role in the biological response induced by nanoparticles (NPs). The acellular cytochrome c oxidation assay has been proposed to determine the intrinsic oxidant-generating capacity of NPs. Yet, there is a need to improve this method to allow a rapid screening to classify NPs in terms of toxicity. We adapted the cytochrome c assay to take into account NP interference, to improve its sensitivity and to develop a high-throughput method. The intrinsic oxidative ability of a panel of NPs (carbon black, MnO, Cu, Ag, BaSO, CeO, TiO and ZnO) was measured with this enhanced test and compared to other acellular redox assays. To assess whether their oxidative potential correlates with cellular responses, we studied the effect of insoluble NPs on the human bronchial epithelial cell line NCI-H292 by measuring the cytotoxicity (WST-1 assay), pro-inflammatory response (IL-8 cytokine production and expression) and antioxidant defense induction (SOD2 and HO-1 expression). The adapted cytochrome c assay had a greatly increased sensitivity allowing the ranking of NPs in terms of their oxidative potential by using the developed high-throughput technique. Besides, a high oxidative potential revealed to be predictive for toxic effects as MnO NPs induced a strong oxidation of cytochrome c and a dose-dependent cytotoxicity, pro-inflammatory response and antioxidant enzyme expression. BaSO, which presented no intrinsic oxidative potential, had no cellular effects. Nevertheless, CeO and TiO NPs demonstrated no acellular oxidant-generating capacity but induced moderate cellular responses. In conclusion, the novel cytochrome c oxidation assay could be used for high-throughput screening of the intrinsic oxidative potential of NPs. However, acellular redox assays may not be sufficient to discriminate among low-toxicity NPs, and additional tests are thus needed.

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Effect of the near-infrared activated photocatalyst Cu2(OH)PO4 nanoparticles on the growth of harmful algal blooms causing Microcystis aeruginosa

Asgodom

Liu

et al. 2021

Environ Sci Pollut Res

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Portable oxidative stress sensor: Dynamic and non-invasive measurements of extracellular H 2 O 2 released by algae

Cited by 15 publications

References 35 publications

Non-invasive continuous monitoring of pro-oxidant effects of engineered nanoparticles on aquatic microorganisms

Non-invasive continuous monitoring of pro-oxidant effects of engineered nanoparticles on aquatic microorganisms

Assessment of the oxidative potential of nanoparticles by the cytochrome c assay: assay improvement and development of a high-throughput method to predict the toxicity of nanoparticles

Effect of the near-infrared activated photocatalyst Cu2(OH)PO4 nanoparticles on the growth of harmful algal blooms causing Microcystis aeruginosa

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