Portable Nanofiber-Light Addressable Potentiometric Sensor  for Rapid <i>Escherichia coli</i> Detection in Orange Juice

Shaibani, Parmiss Mojir; Etayash, Hashem; Jiang, Keren; Sohrabi, Amirreza; Hassanpourfard, Mahtab; Naicker, Selvaraj; Sadrzadeh, Mohtada; Thundat, Thomas

doi:10.1021/acssensors.8b00063

Cited by 74 publications

(41 citation statements)

References 30 publications

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“…This decrease may be because the addition of virus solution not only creates hindrance of the electronic movement of the electrode but also increases solution resistance ( R sol ), decreasing the overall electroactivity. Thereafter, the current intensities decreased gradually when increasing concentrations of the viruses were added onto the surface of the electrode, which indicates that the attachment of virus molecules restricted the electron transfer process 38–40 .…”

Section: Resultsmentioning

confidence: 99%

Electrical pulse-induced electrochemical biosensor for hepatitis E virus detection

et al. 2019

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Hepatitis E virus (HEV) is one of the leading causes of acute viral hepatitis worldwide. In this work, a pulse-triggered ultrasensitive electrochemical sensor was fabricated using graphene quantum dots and gold-embedded polyaniline nanowires, prepared via an interfacial polymerization and then self-assembly approach. Introducing an external electrical pulse during the virus accumulation step increases the sensitivity towards HEV due to the expanded surface of the virus particle as well as the antibody-conjugated polyaniline chain length, compared to other conventional electrochemical sensors. The sensor was applied to various HEV genotypes, including G1, G3, G7 and ferret HEV obtained from cell culture supernatant and in a series of fecal specimen samples collected from G7 HEV-infected monkey. The sensitivity is similar to that detected by real-time quantitative reverse transcription-polymerase chain (RT-qPCR). These results suggests that the proposed sensor can pave the way for the development of robust, high-performance sensing methodologies for HEV detection.

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Section: Resultsmentioning

confidence: 99%

Electrical pulse-induced electrochemical biosensor for hepatitis E virus detection

et al. 2019

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“…As the sensitive layer, electrospun pH-sensitive poly (vinyl alcohol)/poly(acrylic acid) (PVA/PAA) hydrogel NFs was chosen. A limit of detection of 100 CFU/mL was obtained selectively in less than one hour by using NF-LAPS [86].…”

Section: Potentiometric-based Portable Baiosensorsmentioning

confidence: 99%

“…Schematic illustration of the nanofiber-light addressable potentiometric sensor (NF-LAPS) sensor comprising a three-electrode system[86].…”

mentioning

confidence: 99%

Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview

et al. 2020

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Escherichia coli O157:H7 (E. coli O157:H7) is a pathogenic strain of Escherichia coli which has issued as a public health threat because of fatal contamination of food and water. Therefore, accurate detection of pathogenic E. coli is important in environmental and food quality monitoring. In spite of their advantages and high acceptance, culture-based methods, enzyme-linked immunosorbent assays (ELISAs), polymerase chain reaction (PCR), flow cytometry, ATP bioluminescence, and solid-phase cytometry have various drawbacks, including being time-consuming, requiring trained technicians and/or specific equipment, and producing biological waste. Therefore, there is necessity for affordable, rapid, and simple approaches. Electrochemical biosensors have shown great promise for rapid food- and water-borne pathogen detection. Over the last decade, various attempts have been made to develop techniques for the rapid quantification of E. coli O157:H7. This review covers the importance of E. coli O157:H7 and recent progress (from 2015 to 2020) in the development of the sensitivity and selectivity of electrochemical sensors developed for E. coli O157:H7 using different nanomaterials, labels, and electrochemical transducers.

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“…Shaibani et al has described a portable nanofiber-light addressable potentiometric sensor for detection of E . coli with a LOD of 100 CFU/ml within only 1 hour [21]. (c) Impedimetric sensors.…”

Section: Introductionmentioning

confidence: 99%

A microfluidic based biosensor for rapid detection of Salmonella in food products

et al. 2019

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An impedance based microfluidic biosensor for simultaneous and rapid detection of Salmonella serotypes B and D in ready-to-eat (RTE) Turkey matrix has been presented. Detection of Salmonella at a concentration as low as 300 cells/ml with a total detection time of 1 hour has been achieved. The sensor has two sensing regions, with each formed from one interdigitated electrode array (IDE array) consisting of 50 finger pairs. First, Salmonella antibody type B and D were prepared and delivered to the sensor to functionalize each sensing region without causing any cross contamination. Then the RTE Turkey samples spiked with Salmonella types B and D were introduced into the biosensor via the antigen inlet. The response signal resulted from the binding between Salmonella and its specific antibody demonstrated the sensor’s ability to detect a single type of pathogen, and multiple pathogens simultaneously. In addition, the biosensor’s selectivity was tested using non-specific binding of E . coli O157 and E . coli DH5 Alpha while the IDE array was coated with the Salmonella antibody. The results also showed the sensor is capable to differentiate low concentration of live Salmonella cells from high concentration of dead Salmonella cells, and high concentration of E . coli cells. A detailed study on antibody immobilization that includes antibody concentration, antibody coating time (0.5–3 hours) and use of cross-linker has been performed. The study showed that Salmonella antibody to Salmonella antigen is not a factor of antibody concentration after electrodes were saturated with antibody, while the optimal coating time was found to be 1.5 hours, and the use of cross-linker has improved the signal response by 45–60%.

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Portable Nanofiber-Light Addressable Potentiometric Sensor for Rapid Escherichia coli Detection in Orange Juice

Cited by 74 publications

References 30 publications

Electrical pulse-induced electrochemical biosensor for hepatitis E virus detection

Electrical pulse-induced electrochemical biosensor for hepatitis E virus detection

Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview

A microfluidic based biosensor for rapid detection of Salmonella in food products

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