Porous silicon surfaces – A candidate substrate for reverse protein arrays in cancer biomarker detection

Ressine, Anton; Corin, Irina; Järås, Kerstin; Guanti, Ginevra; Simone, Cristiano; Markó-Varga, György; Laurell, Thomas

doi:10.1002/elps.200700379

Cited by 29 publications

(27 citation statements)

References 62 publications

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“…For example, improvements have been reported concerning the substratum and data capture. Functionalized glass (36,37), hydrogel (38,39), PVDF (40,41), macroporous silicon (42), nitrocellulose polymers (43,44) (Grace Bio-Labs; Maine Manufacturing; Sartorius), and planar wave guide surfaces (ZEPTOSENS) (45) have all been successfully implemented to improve sensitivity, spot morphology, precision, and accuracy. Further marked improvements have been made in informatics approaches to deal with sample handling, regional staining correction, quality control, and the identification of high-quality samples and reagents.…”

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confidence: 99%

Realizing the Promise of Reverse Phase Protein Arrays for Clinical, Translational, and Basic Research: A Workshop Report

Akbani

Becker

Carragher

et al. 2014

Molecular & Cellular Proteomics

154

132

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Reverse phase protein array (RPPA) technology introduced a miniaturized "antigen-down" or "dot-blot" immunoassay suitable for quantifying the relative, semi-quantitative or quantitative (if a well-accepted reference standard exists) abundance of total protein levels and post-translational modifications across a variety of biological samples including cultured cells, tissues, and body fluids. The recent evolution of RPPA combined with more sophisticated sample handling, optical detection, quality control, and better quality affinity reagents provides exquisite sensitivity and high sample throughput at a reasonable cost per sample. This facilitates large-scale multiplex analysis of multiple post-translational markers across samples from in vitro, preclinical, or clinical samples. The technical power of RPPA is stimulating the application and widespread adoption of RPPA methods within academic, clinical, and industrial research laboratories.Advances in RPPA technology now offer scientists the opportunity to quantify protein analytes with high precision, sensitivity, throughput, and robustness. As a result, adopters of RPPA technology have recognized critical success factors for useful and maximum exploitation of RPPA technologies, including the following:• preservation and optimization of pre-analytical sample quality,• application of validated high-affinity and specific antibody (or other protein affinity) detection reagents,• dedicated informatics solutions to ensure accurate and robust quantification of protein analytes, and• quality-assured procedures and data analysis workflows compatible with application within regulated clinical environments.In 2011, 2012, and 2013, the first three Global RPPA workshops were held in the United States, Europe, and Japan, respectively. These workshops provided an opportunity for RPPA laboratories, vendors, and users to share and discuss results, the latest technology platforms, best practices, and future challenges and opportunities. The outcomes of the workshops included a number of key opportunities to advance the RPPA field and provide added benefit to existing and future participants in the RPPA research community. The purpose of this report is to share and disseminate, as a community, current knowledge and future directions of the RPPA technology. Molecular & Cellular Proteomics 13: 10.1074/mcp.O113.034918, 1625-1643, 2014.Reverse phase protein array (RPPA) 1 technology represents a highly efficient and cost-effective descendent of miniaturized immunoassays that use a sandwich format for antigen capture (1, 2). Immunoassay arrays were generally sandwichstyle assays in which a series of antibodies immobilized on the solid phase were used to capture the analyte of interest ("antibody capture"), with a second antibody, directed against a different epitope on the same protein, used as a detection molecule. In contrast, in "reverse phase" the analytes (antigens) are immobilized on the solid phase (usually nitrocellulose) and subsequently probed with an antibody or other affini...

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mentioning

confidence: 99%

Realizing the Promise of Reverse Phase Protein Arrays for Clinical, Translational, and Basic Research: A Workshop Report

Akbani

Becker

Carragher

et al. 2014

Molecular & Cellular Proteomics

154

132

View full text Add to dashboard Cite

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“…In order to improve biomolecules loading capacity on the solid substrate, one straightforward approach is to increase the surface area of the substrate by enhancing the surface roughness (Nijdam et al 2007;Ressine et al 2007, Vlachopoulou et al 2009). Therefore, the significant increase in roughness of PDMS artificial lotus leaf substrate can provide larger surface areas for great improvement of protein loading capacity in immunoassay.…”

Section: Immunoassay On Microfluidic Chip With 3d Structuresmentioning

confidence: 99%

Rapid fabrication of microfluidic chip with three-dimensional structures using natural lotus leaf template

Liu

2010

Microfluid Nanofluid

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This article describes a simple and rapid fabrication method for microfluidic chip with three-dimensional (3D) structures by using natural lotus leaf as a template. This microfluidic chip consists of one PDMS artificial lotus leaf substrate with micro/nano hierarchical structure and one PDMS replica with microfluidic network. PDMS artificial lotus leaf substrate is directly replicated from natural lotus leaf template by soft lithography technology. In order to seal this rough PDMS artificial lotus leaf substrate against PDMS replica, a PDMS adhesiveassisted bonding method is developed to assist the sealing of our microfluidic chip. Compared to previously reported microfluidic chips with 3D structures based on MEMS/ NEMS technology, the rapid fabrication of our microfluidic chip against a natural lotus leaf template is simple, low cost, easy to control, and highly faithful without the need of expensive MEMS facility and clean room condition. As an application demonstration, we fabricate a microfluidic immunosensing chip for C-reactive protein (CRP) detection by taking advantage of its high surface area. The experiment result shows that our microfluidic chip with 3D structures has more than five times higher sensitivity than those obtained from the conventional microfluidic chip with a flat channel wall due to the increase of the surface area and consequent increase of the amount of immobilized antibody. The present microfluidic chip with 3D structures also can be applied in proteins microarray, cell culture, cell adhesion, and so on.

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“…Unique fluorescence properties and tremendous surface areas of porous silicon (500-800 m 2 /g) made it an ideal candidate for protein sensors (Sailor, 2007). Ressine et al (2007) used porous silicon as a scaffold for antibody arrays and obtained detection limits down to 1 picomolar for IgG and 20 picomolar for prostatespecific antigen (PSA) in clinical samples. Hill et al (2009) explored the curvature effect of spherical gold nanoparticles on the loading density of probes.…”

Section: Nanostructured Surfacesmentioning

confidence: 99%

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Kobeissy

Gülbakan

Alawieh

et al. 2014

OMICS: A Journal of Integrative Biology

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The post-genomics era has brought about new Omics biotechnologies, such as proteomics and metabolomics, as well as their novel applications to personal genomics and the quantified self. These advances are now also catalyzing other and newer post-genomics innovations, leading to convergences between Omics and nanotechnology. In this work, we systematically contextualize and exemplify an emerging strand of post-genomics life sciences, namely, nanoproteomics and its applications in health and integrative biological systems. Nanotechnology has been utilized as a complementary component to revolutionize proteomics through different kinds of nanotechnology applications, including nanoporous structures, functionalized nanoparticles, quantum dots, and polymeric nanostructures. Those applications, though still in their infancy, have led to several highly sensitive diagnostics and new methods of drug delivery and targeted therapy for clinical use. The present article differs from previous analyses of nanoproteomics in that it offers an in-depth and comparative evaluation of the attendant biotechnology portfolio and their applications as seen through the lens of post-genomics life sciences and biomedicine. These include: (1) immunosensors for inflammatory, pathogenic, and autoimmune markers for infectious and autoimmune diseases, (2) amplified immunoassays for detection of cancer biomarkers, and (3) methods for targeted therapy and automatically adjusted drug delivery such as in experimental stroke and brain injury studies. As nanoproteomics becomes available both to the clinician at the bedside and the citizens who are increasingly interested in access to novel post-genomics diagnostics through initiatives such as the quantified self, we anticipate further breakthroughs in personalized and targeted medicine.

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Porous silicon surfaces – A candidate substrate for reverse protein arrays in cancer biomarker detection

Cited by 29 publications

References 62 publications

Realizing the Promise of Reverse Phase Protein Arrays for Clinical, Translational, and Basic Research: A Workshop Report

Realizing the Promise of Reverse Phase Protein Arrays for Clinical, Translational, and Basic Research: A Workshop Report

Rapid fabrication of microfluidic chip with three-dimensional structures using natural lotus leaf template

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

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