Porous Silicon Nanodiscs for Targeted Drug Delivery

Abstract

International audienc

Dual‐Action Cancer Therapy with Targeted Porous Silicon Nanovectors

“…Thus, nanoparticle (NP)‐based delivery platforms capable of combining hyperthermia with traditional chemotherapeutics may afford the efficient treatment of highly drug‐resistant tumors . Moreover, the vectorization of therapeutics using NPs that actively target membrane receptors overexpressed in cancer cells has been recently proposed as an efficient way to guarantee selective payload delivery and therefore improve therapeutic outcomes …”

“…Porous silicon (pSi) NPs are i) biodegradable, ii) suitable for conjugation with targeting moieties, and iii) excellent carriers of chemotherapy drugs . Here, we utilized these unique properties of pSiNPs and loaded them with two different therapeutics, while immobilizing cell‐specific antibodies to ensure active targeting ( Scheme ).…”

“…pSiNPs were fabricated by electrochemical etching of crystalline Si as reported previously by our group. [4b] Figure S1A (Supporting Information) shows a scanning electron microscopy image of the perforated structure which has a thickness of 187 ± 16 nm. The anodized porous layer (Figure S1B, Supporting Information) was lifted off the Si wafer by electropolishing and fractured into particles by ultrasonication.…”

“…Active targeting strategies have an impact on nanoparticle–cell interactions and particle internalization. [4a,b] To add the targeting function to pSiNPs, a cell‐targeting antibody (Ab) was immobilized to the NP surface. We used semicarbazide chemistry, which allows the conjugation of the periodate‐oxidized Ab through the Fc (Fragment, crystallizable) region resulting in specifically oriented binding.…”

“…We used semicarbazide chemistry, which allows the conjugation of the periodate‐oxidized Ab through the Fc (Fragment, crystallizable) region resulting in specifically oriented binding. [4a] Freshly prepared pSiNPs were functionalized with tert ‐butyl‐2[(allylamino)carbonyl]hydrazine‐carboxylate (SC) by thermal hydrosilylation. Prior to Ab immobilization, the tert ‐butyloxycarbonyl‐protected group in the SC moiety was removed.…”