Inhibition of Multidrug Resistance of Cancer Cells by Co‐Delivery of DNA Nanostructures and Drugs Using Porous Silicon Nanoparticles@Giant Liposomes

Kong, Feng; Zhang, Xu; Zhang, Hongbo; Qu, Xiangmeng; Chen, Dong; Servos, Mark R.; Mäkilä, Ermei; Salonen, Jarno; Santos, Hélder A.; Ming, Hai; Weitz, David A.

doi:10.1002/adfm.201500594

Cited by 116 publications

(79 citation statements)

References 48 publications

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“…Thus, novel nanoparticle drug delivery technologies are being aggressively developed. Structural DNA nanotechnology, which enables unprecedented control over nanoscale geometry and biochemical functionalization, has recently demonstrated immense potential for biomedical applications including controlled release of medicinal compounds, fluorescent‐based imaging applications, targeted delivery, and particularly relevant to this study, delivery of small molecule chemical therapeutics …”

Section: Introductionmentioning

confidence: 99%

Daunorubicin‐Loaded DNA Origami Nanostructures Circumvent Drug‐Resistance Mechanisms in a Leukemia Model

Halley

Lucas

McWilliams

et al. 2015

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Many cancers show primary or acquired drug resistance due to the overexpression of efflux pumps. A novel mechanism to circumvent this is to integrate drugs, such as anthracycline antibiotics, with nanoparticle delivery vehicles that can bypass intrinsic tumor drug-resistance mechanisms. DNA nanoparticles serve as an efficient binding platform for intercalating drugs (e.g. anthracyclines doxorubicin and daunorubicin, which are widely used to treat acute leukemias) and enable precise structure design and chemical modifications, for example for incorporating targeting capabilities. Here, we utilize DNA nanostructures to circumvent daunorubicin drug resistance at clinically relevant doses in a leukemia cell line model. We report the fabrication of a rod-like DNA origami drug carrier that can be controllably loaded with daunorubicin. We further directly verify that nanostructure-mediated daunorubicin delivery leads to increased drug entry and retention in cells relative to free daunorubicin at equal concentrations, which yields significantly enhanced drug efficacy. Our results indicate that DNA origami nanostructures can circumvent efflux pump-mediated drug resistance in leukemia cells at clinically relevant drug concentrations and provide a robust DNA nanostructure design that could be implemented in a wide range of cellular applications due to its remarkably fast self-assembly (~5 minutes) and excellent stability in cell culture conditions.

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Section: Introductionmentioning

confidence: 99%

Daunorubicin‐Loaded DNA Origami Nanostructures Circumvent Drug‐Resistance Mechanisms in a Leukemia Model

Halley

Lucas

McWilliams

et al. 2015

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205

163

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“…Since the first liposomes were fabricated, researchers have done a series of investigations about the biophysical and biochemical properties of liposomes. For example, liposomes were utilized as a model to explore cell membranes, these nanoparticles were also used in the areas of drug delivery [6], diagnostics [7], imaging [8], food and cosmetic industries [9,10], molecular biology [11], biochemistry [12], and microfluidics [13].…”

Section: Liposomesmentioning

confidence: 99%

Advanced liposome-loaded scaffolds for therapeutic and tissue engineering applications

et al. 2020

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Liposome is one of the most commonly used drug delivery systems in the world, due to its excellent biocompatibility, satisfactory ability in controlling drug release, and passive targeting capability. However, some drawbacks limit the application of liposomes in clinical, such as problems in transporting, storing, and difficulties in maintaining the drug concentration in the local area. Scaffolds usually are used as implants to supply certain mechanical supporting to the defective area or utilized as diagnosis and imaging methods. But, in general, unmodified scaffolds show limited abilities in promoting tissue regeneration and treating diseases. Therefore, liposome-scaffold composite systems are designed to take advantages of both liposomes' biocompatibility and scaffolds' strength to provide a novel system that is more suitable for clinical applications. This review introduces and discusses different types of liposomes and scaffolds, and also the application of liposome-scaffold composite systems in different diseases, such as cancer, diabetes, skin-related diseases, infection and human immunodeficiency virus, and in tissue regeneration like bone, teeth, spinal cord and wound healing.Journal Pre-proof Drug-loaded liposomesLiposomes have been widely investigated as drug carriers, from the approved amphotericin B liposome in 1990 to the successful Onivyde™ in 2015. As drug carriers, liposomes have several advantages, for instance, they can carry various types of drugs, specifically, the internal water core can load hydrophilic drugs and the bilayers can carry hydrophobic ones. Additionally, liposomes exhibit the ability in controlling the drug release and decreasing the side effect of drugs. Furthermore, liposomes also show advantages in low toxicity, non-immunogenic and biodegradability. The phospholipid bilayer can be regarded as satisfactory platforms that can be modified with diverse ligands to achieve the goal of targeting delivery. Furthermore, the stability and efficacy of biological products can also be improved by utilizing liposomes as vehicles. Delivering small molecule hydrophilic drugsSeveral methods, such as reverse evaporation, pH gradient, ammonium sulfate gradient and repeated freeze thawing, are usually applied in the process of preparing small hydrophilic drugs-loaded liposomes. For example, Takeuchi et al. [14] fabricated polyborane encapsulated liposomes though pH gradient or reverse-phase evaporation, then they found that for the encapsulation efficiency of the liposome Journal Pre-proof 6 prepared using the pH gradient was twice as high as that prepared, using reverse-phase evaporation. Additionally, they observed that boron concentration of the polyborane encapsulated liposomes prepared using the pH gradient achieved 110-150 μg/g of tumor tissue. Delivering small molecule hydrophobic drugsUsually the film dispersion method is one of the simplest ways to fabricate lipid drug-loaded liposomes. For example, Fu et al.[15] fabricated novel temperature-sensitive liposomes loading paclitaxel (PTX-...

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Section: Ferrofluid‐based Detection Diagnosis and Therapymentioning

confidence: 99%

“…Magnetoliposomes are found to be another ideal drug delivery vehicle, of which ferromagnetic particles and hydrophilic drugs are encapsulated in aqueous cores, while hydrophobic drugs are trapped in lipid bilayers . As shown in Figure c, a giant multifunctional liposome was developed by Kong et al Doxorubicin hydrochloride (DOX), DNA nanostructure, Erlotinib‐loaded porous silicon nanoparticles, ferromagnetic particles, along with gold nanorods were all encapsulated in the cores of the giant liposomes, and the hydrophobic Telatinib was encapsulated in their lipid bilayers. Loading with these drugs, nanoparticles, and biomolecules, the resultant giant liposomes not only enabled codelivery of multiple antitumor drugs, avoided multidrug resistance, achieved synergistic antitumor effects, but also combined photothermal effects, magnetic targeting, and magnetothermal effects.…”

Section: Ferrofluid‐based Detection Diagnosis and Therapymentioning

confidence: 99%

Flexible Ferrofluids: Design and Applications

et al. 2019

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Ferrofluids, also known as ferromagnetic particle suspensions, are materials with an excellent magnetic response, which have attracted increasing interest in both industrial production and scientific research areas. Because of their outstanding features, such as rapid magnetic reaction, flexible flowability, as well as tunable optical and thermal properties, ferrofluids have found applications in various fields, including material science, physics, chemistry, biology, medicine, and engineering. Here, a comprehensive, in‐depth insight into the diverse applications of ferrofluids from material fabrication, droplet manipulation, and biomedicine to energy and machinery is provided. Design of ferrofluid‐related devices, recent developments, as well as present challenges and future prospects are also outlined.

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Inhibition of Multidrug Resistance of Cancer Cells by Co‐Delivery of DNA Nanostructures and Drugs Using Porous Silicon Nanoparticles@Giant Liposomes

Cited by 116 publications

References 48 publications

Daunorubicin‐Loaded DNA Origami Nanostructures Circumvent Drug‐Resistance Mechanisms in a Leukemia Model

Daunorubicin‐Loaded DNA Origami Nanostructures Circumvent Drug‐Resistance Mechanisms in a Leukemia Model

Advanced liposome-loaded scaffolds for therapeutic and tissue engineering applications

Flexible Ferrofluids: Design and Applications

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