Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect

Wang, Xing; Wu, Xia; Huan, Xing; Zhang, Guilan; Shi, Quan; Lingling, E; Liu, Na; Yang, Tingyuan; Wang, Dongsheng; Qi, Feng; Wang, Lianyan; Liu, Hongchen

doi:10.1021/acsami.6b13566

Cited by 59 publications

(45 citation statements)

References 47 publications

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“…Furthermore, the study of growth signaling has become important for growth of alveolar, maxillary, and mandibular TE constructs. A plethora of graft biomaterials and biochemical factors have been studied with the goal of improving the tissue‐engineered construct's scaffold integration and tissue growth 48‐58,66‐79 …”

Section: Discussionmentioning

confidence: 99%

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Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

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Section: Discussionmentioning

confidence: 99%

“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate craniofacial tissue engineering 49‐72,74‐77,79,81‐84,145‐197 …”

Section: Discussionmentioning

confidence: 99%

Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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“…The evaluation was performed using three types of particles: nanospheres of 121.62 ± 2.5 nm, microspheres of 1.61 ± 0.08 µm, and 10× microspheres of 21.45 ± 0.22 µm. As a result, ALP and OCN expression from BMSCs was the highest in the microspheres group, indicating a strong differentiation into bone tissue [113]. In an in vivo study, the repair of critical size bone defects were evaluated in rabbit mandibles, and the greatest bone formation was similarly found in the microspheres group.…”

Section: Structure Of Scaffoldsmentioning

confidence: 98%

Biodegradable Polymers as Drug Delivery Systems for Bone Regeneration

Aoki

2020

Pharmaceutics

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Regenerative medicine has been widely researched for the treatment of bone defects. In the field of bone regenerative medicine, signaling molecules and the use of scaffolds are of particular importance as drug delivery systems (DDS) or carriers for cell differentiation, and various materials have been explored for their potential use. Although calcium phosphates such as hydroxyapatite and tricalcium phosphate are clinically used as synthetic scaffold material for bone regeneration, biodegradable materials have attracted much attention in recent years for their clinical application as scaffolds due their ability to facilitate rapid localized absorption and replacement with autologous bone. In this review, we introduce the types, features, and performance characteristics of biodegradable polymer scaffolds in their role as DDS for bone regeneration therapy.

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“…The amount of insulin loaded into the nHAC scaffolds was determined by subtracting the remaining insulin from the initial amount. 14 Measurements were carried out three times.…”

Section: Fabrication Of Nhac/plga Composite Scaffoldsmentioning

confidence: 99%

Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold

Wang¹,

Zhang²,

Qi³

et al. 2017

IJN

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Insulin is widely considered as a classical hormone and drug in maintaining energy and glucose homeostasis. Recently, insulin has been increasingly recognized as an indispensable factor for osteogenesis and bone turnover, but its applications in bone regeneration have been restricted because of the short periods of activity and uncontrolled release. In this study, we incorporated insulin-loaded poly lactic-co-glycolic-acid (PLGA) nanospheres into nano-hydroxyapatite/collagen (nHAC) scaffolds and investigated the bioactivity of the composite scaffolds in vitro and in vivo. Bioactive insulin was successfully released from the nanospheres within the scaffold, and the release kinetics of insulin could be efficiently controlled by uniform-sized nanospheres. The physical characterizations of the composite scaffolds demonstrated that incorporation of nanospheres in nHAC scaffolds using this method did not significantly change the porosity, pore diameters, and compressive strengths of nHAC. In vitro, the insulin-loaded nHAC/PLGA composite scaffolds possessed favorable biological function for bone marrow mesenchymal stem cells adhesion and proliferation, as well as the differentiation into osteoblasts. In vivo, the optimized bone regenerative capability of this composite scaffold was confirmed in rabbit mandible critical size defects. These results demonstrated successful development of a functional insulin–PLGA–nHAC composite scaffold that enhances the bone regeneration capability of nHAC.

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Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect

Cited by 59 publications

References 47 publications

Tissue engineering applications in otolaryngology—The state of translation

Tissue engineering applications in otolaryngology—The state of translation

Biodegradable Polymers as Drug Delivery Systems for Bone Regeneration

Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold

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