Porous membrane with reverse gradients of PDGF-BB and BMP-2 for tendon-to-bone repair: In vitro evaluation on adipose-derived stem cell differentiation

Min, Hyun Ki; Oh, Se Heang; Lee, Jong Min; Im, Gun‐Il; Lee, Jin Ho

doi:10.1016/j.actbio.2013.12.031

Cited by 53 publications

(61 citation statements)

References 36 publications

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“…In a previously mentioned study [110], the authors investigated the effect of PDGF-BB and BMP-2 reverse gradients on the proliferation and differentiation of ASCs for tendon-to-bone repair. Growth factor gradients were generated by immobilizing heparin on a PCL-Pluronic Tissue vascularization and oxygen concentration are other important parameters to be taken into account.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Recent studies report the feasibility of using ASCs for the generation of bone- [35, 37, 42, 43, [110] seeded ASCs on a PCL porous membrane loaded with a reverse gradient of platelet-derived growth factor B (PDGF-BB) and BMP-2 to generate a tendon-to-bone construct. This strategy enabled the generation of a gradient of molecular stimuli that resulted in specific ASC differentiation depending on growth factor concentration.…”

Section: Cell-and Culture-based Strategiesmentioning

confidence: 99%

See 1 more Smart Citation

Strategies to engineer tendon/ligament-to-bone interface: Biomaterials, cells and growth factors

Tellado

Balmayor

Griensven

2015

Advanced Drug Delivery Reviews

218

216

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Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Cell-and Culture-based Strategiesmentioning

confidence: 99%

Strategies to engineer tendon/ligament-to-bone interface: Biomaterials, cells and growth factors

Tellado

Balmayor

Griensven

2015

Advanced Drug Delivery Reviews

218

216

View full text Add to dashboard Cite

“…6C). [144] In combination with materials processing methods and cells, growth factor gradients contribute to the development of integrated and graded regions for engineered soft tissue-to-bone interfaces.…”

Section: Biochemical Factorsmentioning

confidence: 99%

“…Reprinted from Acta Biomaterialia, Volume 10, Issue 3, Hyun Ki Min, Se Heang Oh, Jonh Min Lee, Gun Il Im, Jin Ho Lee, Porous membrane with reverse gradients of PDGF-BB and BMP-2 for tendon-to-bone repair: In vitro evaluation on adipose-derived stem cell differentiation, 1272 – 1279, Copyright © 2013, with permission from Elsevier. [144] …”

Section: Figmentioning

confidence: 99%

Next generation tissue engineering of orthopedic soft tissue-to-bone interfaces

et al. 2017

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Soft tissue-to-bone interfaces are complex structures that consist of gradients of extracellular matrix materials, cell phenotypes, and biochemical signals. These interfaces, called entheses for ligaments, tendons, and the meniscus, are crucial to joint function, transferring mechanical loads and stabilizing orthopedic joints. When injuries occur to connected soft tissue, the enthesis must be re-established to restore function, but due to structural complexity, repair has proven challenging. Tissue engineering offers a promising solution for regenerating these tissues. This prospective review discusses methodologies for tissue engineering the enthesis, outlined in three key design inputs: materials processing methods, cellular contributions, and biochemical factors.

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“…Moreover, many common techniques to repair tendon tears at the tendon-bone interface, such as the use of suture anchors, cannot regenerate the enthesis. The tendon and bone are thus only held together primarily by sutures, resulting in a high incidence of rerupture after tendon reattachment surgeries [7,8]. Therefore, alternative surgical procedures may be required to ensure proper tendon healing.…”

Section: Introductionmentioning

confidence: 99%

Biologic and Tissue Engineering Strategies for Tendon Repair

Sigal

Grande

Dines

et al. 2016

Regen. Eng. Transl. Med.

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This review summarizes recent developments in biologic treatments-including growth factors, platelet-rich plasma (PRP), stem cells, and cell-seeded scaffolds-for tendon repair. Growth and differentiation faction-5 (GDF-5), insulin-related growth factor-1 (IGF-1), and basic fibroblast growth factor (bFGF) all improved extracellular matrix (ECM) production and tensile strength of treated tendons; however, no clinical trials were done on GDF-5. Platelet-derived growth factor-BB (PDGF-BB) improved proliferation and ECM production, but did not consistently improve mechanical properties. The literature was mixed on the efficacy of PRP for the treatment of chronic and acute tendinopathies. However, PRP did cause any complications, and its benefits may be enhanced once an ideal, standardized composition is developed. Therefore, PRP may be a valid treatment, especially once nonsurgical management options have failed. Mesenchymal stem cells (MSCs) significantly and substantially improved the quality of tendon repairs and demonstrated the ability to regenerate an enthesis. Adipose-derived stem cells (ADSCs) have similar effects and are easier to harvest. The periosteum may also regenerate the tendon-bone attachment. Tenocytes, meanwhile, may be ideal for midsubstance tendon repairs. Cell-seeded scaffolds-especially ECMderived scaffolds-were demonstrated to improve ECM production, enhancing the healing abilities of tenocytes or stem cells while providing early mechanical support to healing tendons.Each of these treatments demonstrated enhanced healing compared to common surgical techniques; moreover, patient outcomes may be enhanced by combining these treatments. Lay SummaryTendon injuries are very prevalent and can be debilitating. Tendon heals poorly, and the scar tissue that forms is weak and susceptible to reinjury. A major focus of orthopedic research is regenerative medicine, encouraging the formation of healthy tendon rather than mechanically inferior scar tissue. This review summarizes the recent scientific literature on biologic treatments for tendon repair, such as growth factors, platelet-rich plasma, and stem cells. The purpose of which is to show which treatments are promising candidates for clinical use and research, helping to guide physicians and to lay out a path for future research.

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Porous membrane with reverse gradients of PDGF-BB and BMP-2 for tendon-to-bone repair: In vitro evaluation on adipose-derived stem cell differentiation

Cited by 53 publications

References 36 publications

Strategies to engineer tendon/ligament-to-bone interface: Biomaterials, cells and growth factors

Strategies to engineer tendon/ligament-to-bone interface: Biomaterials, cells and growth factors

Next generation tissue engineering of orthopedic soft tissue-to-bone interfaces

Biologic and Tissue Engineering Strategies for Tendon Repair

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