Porous calcium phosphate–collagen composite microspheres for effective growth factor delivery and bone tissue regeneration

Seong, Yun‐Jeong; Song, Eun Kee; Park, Cheonil; Lee, Hyun; Kang, In-Ku; Kim, Hyoun‐Ee; Jeong, Sunjoo

doi:10.1016/j.msec.2019.110480

Cited by 36 publications

(21 citation statements)

References 33 publications

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“…Collagen type I or hydroxyapatite have been used in order to improve resorbable scaffold biocompatibility and promote bone tissue regeneration. Both collagen type I and HAp are well known for their osteogenic potential [ 30 , 31 , 32 ]. It was already evidenced by Lee et al [ 33 ], that the adhesion of hMSC was significantly improved upon coating of polylactide surface with collagen.…”

Section: Discussionmentioning

confidence: 99%

Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits

Krok-Borkowicz

Reczyńska

Rumian

et al. 2020

IJMS

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Poly(l-lactide-co-glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and bone tissue regeneration potential. The scaffolds were fabricated using solvent casting/particulate leaching method. Their total porosity was 85% and the pore size was in the range of 250–320 µm. The physico-chemical properties of the scaffolds were evaluated using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), sessile drop, and compression tests. Three types of the scaffolds (unmodified PLGA, PLGA/coll, and PLGA/HAp) were implanted into the defects created in New Zealand rabbit femoral trochlears; empty defect acted as control. Samples were extracted after 1, 4, 12, and 26 weeks from the implantation, evaluated using micro-computed tomography (µCT), and stained by Masson–Goldner and hematoxylin-eosin. The results showed that the proposed method is suitable for fabrication of highly porous PLGA scaffolds. Effective deposition of both coll and HAp was confirmed on all surfaces of the pores through the entire scaffold volume. In the in vivo model, PLGA and PLGA/HAp scaffolds enhanced tissue ingrowth as shown by histological and morphometric analyses. Bone formation was the highest for PLGA/HAp scaffolds as evidenced by µCT. Neo-tissue formation in the defect site was well correlated with degradation kinetics of the scaffold material. Interestingly, around PLGA/coll extensive inflammation and inhibited tissue healing were detected, presumably due to immunological response of the host towards collagen of bovine origin. To summarize, PLGA scaffolds modified with HAp are the most promising materials for bone tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits

Krok-Borkowicz

Reczyńska

Rumian

et al. 2020

IJMS

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“…Nevertheless, osteogenic properties of PCL/gelatin membranes are often limited. To impart the electrospun membranes with tissue regeneration ability, bioactive growth factors (GFs), such as bone morphogenetic protein 2 (BMP-2) [ 16 ] and platelet-derived growth factor (PDGF) [ 17 ], were incorporated. However, the short protein half-life, possible denaturation as well as high cost have limited the use of bioactive GFs.…”

Section: Introductionmentioning

confidence: 99%

Cerium oxide nanoparticles loaded nanofibrous membranes promote bone regeneration for periodontal tissue engineering

Ren

Zhou

Zheng

et al. 2022

Bioactive Materials

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Bone regeneration is a crucial part in the treatment of periodontal tissue regeneration, in which new attempts come out along with the development of nanomaterials. Herein, the effect of cerium oxide nanoparticles (CeO 2 NPs) on the cell behavior and function of human periodontal ligament stem cells (hPDLSCs) was investigated. Results of CCK-8 and cell cycle tests demonstrated that CeO 2 NPs not only had good biocompatibility, but also promoted cell proliferation. Furthermore, the levels of alkaline phosphatase activity, mineralized nodule formation and expressions of osteogenic genes and proteins demonstrated CeO 2 NPs could promote osteogenesis differentiation of hPDLSCs. Then we chose electrospinning to fabricate fibrous membranes containing CeO 2 NPs. We showed that the composite membranes improved mechanical properties as well as realized release of CeO 2 NPs. We then applied the composite membranes to in vivo study in rat cranial defect models. Micro-CT and histopathological evaluations revealed that nanofibrous membranes with CeO 2 NPs further accelerated new bone formation. Those exciting results demonstrated that CeO 2 NPs and porous membrane contributed to osteogenic ability, and CeO 2 NPs contained electrospun membrane may be a promising candidate material for periodontal bone regeneration.

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“…The physical characteristics of implanted biomaterials can affect the proliferation rate of osteoblasts and has important effects on cytokine gene expression (Barbeck et al, 2021). It has been established that the porous structure of calcium phosphate material can significantly influence bone regeneration (Xie et al, 2016;Bastami et al, 2017;Seong et al, 2020;Zamani et al, 2021). The porous surface of bone materials enhances the mechanical interlocking between the bone substitute and the surrounding bone tissues, providing mechanical stability to the bone-material interface (Meka et al, 2019;Roopavath et al, 2019;Cao et al, 2019;Dos Santos Trento et al, 2020).…”

Section: The Influence Of Physical Properties Of Beta-tricalcium Phosphate On Osteogenesismentioning

confidence: 99%

Current Application of Beta-Tricalcium Phosphate in Bone Repair and Its Mechanism to Regulate Osteogenesis

Zhou

et al. 2021

Front. Mater.

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Large segmental bone loss and bone resection due to trauma and/or the presence of tumors and cysts often results in a delay in healing or non-union. Currently, the bone autograft is the most frequently used strategy to manage large bone loss. Nevertheless, autograft harvesting has limitations, namely sourcing of autograft material, the requirement of an invasive procedure, and susceptibility to infection. These disadvantages can result in complications and the development of a bone substitute materials offers a potential alternative to overcome these shortcomings. Among the biomaterials under consideration to date, beta-tricalcium phosphate (β-TCP) has emerged as a promising material for bone regeneration applications due to its osteoconductivity and osteoinductivity properties as well as its superior degradation in vivo. However, current evidence suggests the use β-TCP can in fact delay bone healing and mechanisms for this observation are yet to be comprehensively investigated. In this review, we introduce the broad application of β-TCP in tissue engineering and discuss the different approaches that β-TCP scaffolds are customized, including physical modification (e.g., pore size, porosity and roughness) and the incorporation of metal ions, other materials (e.g., bioactive glass) and stem cells (e.g., mesenchymal stem cells). 3D and 4D printed β-TCP-based scaffolds have also been reviewed. We subsequently discuss how β-TCP can regulate osteogenic processes to aid bone repair/healing, namely osteogenic differentiation of mesenchymal stem cells, formation of blood vessels, release of angiogenic growth factors, and blood clot formation. By way of this review, a deeper understanding of the basic mechanisms of β-TCP for bone repair will be achieved which will aid in the optimization of strategies to promote bone repair and regeneration.

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Porous calcium phosphate–collagen composite microspheres for effective growth factor delivery and bone tissue regeneration

Cited by 36 publications

References 33 publications

Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits

Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits

Cerium oxide nanoparticles loaded nanofibrous membranes promote bone regeneration for periodontal tissue engineering

Current Application of Beta-Tricalcium Phosphate in Bone Repair and Its Mechanism to Regulate Osteogenesis

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