Porous biochar/chitosan composites for high performance cellulase immobilization by glutaraldehyde

Mo, Haodao; Qiu, Jianhui; Yang, Chao; Zang, Limin; Sakai, Eiichi; Chen, Jiao

doi:10.1016/j.enzmictec.2020.109561

Cited by 49 publications

(21 citation statements)

References 49 publications

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“…The immobilized cellulase on the biochar/chitosan support showed superior activity and reusability (91 % glucose productivity after 10 cycles) during the formation of glucose (Figure 8) from the enzymatic hydrolysis of carboxymethyl cellulose (CMC). [64] Cellulase solution at pH 4 was added to polyporous biochar (obtained from the thermochemical treatment of lignocellulosic biomass) combined with magnetic γ-Fe 2 O 3 particle crystallized on the pores and at the high surface area (ca. 400 m 2 g À 1 ) of the biochar (obtained by calcination of the Fe(II/III)-containing biochar).…”

Section: Scheme 3 Hydrolysis Of Insoluble Cellulose With Cellulase Into Soluble Glucosementioning

confidence: 99%

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Challenges and Opportunities for the Encapsulation of Enzymes over Porous Solids for Biodiesel Production and Cellulose Valorization into Glucose

Cirujano

Dhakshinamoorthy

2021

ChemCatChem

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enzymes, via simple adsorption or covalent grafting. Herein, we provide a comprehensive review by comparing the catalytic performance of the enzymes supported over porous solids with respect to the bulk/free enzymes in terms of yields, stability and recycling, highlighting the benefits of the porous support for an optimal biocatalytic performance in the biodiesel production and cellulose valorization into glucose. The final section provides our views for further developments in this field.

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Section: Scheme 3 Hydrolysis Of Insoluble Cellulose With Cellulase Into Soluble Glucosementioning

confidence: 99%

“…Influence of the loading of chitosan (CS) during reusability in the glucose production. The numbers 25, 50 and 100 indicate the loading of CS in mg.Reproduced with permission from ref [64]. Copyright 2020 Elsevier.…”

mentioning

confidence: 99%

Challenges and Opportunities for the Encapsulation of Enzymes over Porous Solids for Biodiesel Production and Cellulose Valorization into Glucose

Cirujano

Dhakshinamoorthy

2021

ChemCatChem

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“…In the past few years, cellulases have been successfully immobilized with various methods (Mubarak et al, 2014;Mo et al, 2020). Cellulase immobilization onto coffee surface provides an excellent base for increasing the enzyme productivity and different uses of the enzyme (Buntić et al, 2018).…”

Section: Modify the Enzymes And Increase Their Efficiencymentioning

confidence: 99%

An overview of biomass conversion: exploring new opportunities

Fülöp¹,

Ecker²

2020

PeerJ

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Recycling biomass is indispensable these days not only because fossil energy sources are gradually depleted, but also because pollution of the environment, caused by the increasing use of energy, must be reduced. This article intends to overview the results of plant biomass processing methods that are currently in use. Our aim was also to review published methods that are not currently in use. It is intended to explore the possibilities of new methods and enzymes to be used in biomass recycling. The results of this overview are perplexing in almost every area. Advances have been made in the pre-treatment of biomass and in the diversity and applications of the enzymes utilized. Based on molecular modeling, very little progress has been made in the modification of existing enzymes for altered function and adaptation for the environmental conditions during the processing of biomass. There are hardly any publications in which molecular modeling techniques are used to improve enzyme function and to adapt enzymes to various environmental conditions. Our view is that using modern computational, biochemical, and biotechnological methods would enable the purposeful design of enzymes that are more efficient and suitable for biomass processing.

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“…For an efficient hydrolysis of cellulose-based woody and non-woody biomass in industrial and agricultural applications, active and stable cellulases are needed. Enzymes' stabilization by immobilization increases their usability, as well as the number and diversity of specific applications (Dinçer & Telefoncu, 2007;Das et al, 2011;Yang et al, 2021, Haodao et al, 2020, Zheng et al, 2019.…”

Section: Introductionmentioning

confidence: 99%

“…Only a few articles deal with the entrapment/encapsulation of cellulases in polymeric structures, probably because the low solubility of substrates and low diffusion of cellulose, a macromolecular substrate, in the matrix may diminish the enzyme's activity. Due to the growing interest in efficiently stabilized cellulases in biomass transformation processes, most of such papers were published in recent years (Haodao et al, 2020, Zheng et al, 2019, Ajay & Sangeeta, 2020Wei et al, 2020, Yonca et al, 2020.…”

Section: Introductionmentioning

confidence: 99%

Microbial cellulases immobilized in biopolymer/silica matrices used as enzyme release systems

et al. 2021

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Trichoderma viride CMGB 1 cellulases were immobilized by entrapment in silica gels (by sol-gel method), alginate biopolymers and hybrid alginate/silica materials. Tetramethoxysilane (TMOS), tetraethoxysilane (TEOS) and tetrakis (2-hydroxyethyl) orthosilicate (THEOS) were used as organoalkoxysilane precursors and ethanol or ethylene glycol as cosolvents in a two step sol-gel synthesis. Combined alginate/silica matrices resulted by mixing silica sol with sodium alginate or by coating alginate beads with a silica shell. The partial confinement of ethylene glycol in the matrix with consequences on biocatalytic activity was investigated using SEM-EDAX, thermal analysis and FT-IR spectroscopy. The efficiency of the enzyme-matrix biomaterials was tested in controlled enzyme release experiments. The sol-gel method developed using EG as a co-solvent allowed cellulase immobilization yields 1.5-4.5 times higher compared to classical sol-gel methods that use EtOH. The characterization of the gels by microscopic and spectrophotometric analyzes showed that there are similarities between the structure of the gels based on THEOS and those developed by us from TEOS, TMOS and EG as co-solvent. The new developed gels showed good cellulase release properties at acidic pH, comparable to those based on THEOS and alginate. The microbial cellulases immobilized in the matrices obtained and characterized in this work can operate as efficient systems for releasing enzymes, in acidic pH conditions, as feed additives.

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Porous biochar/chitosan composites for high performance cellulase immobilization by glutaraldehyde

Cited by 49 publications

References 49 publications

Challenges and Opportunities for the Encapsulation of Enzymes over Porous Solids for Biodiesel Production and Cellulose Valorization into Glucose

Challenges and Opportunities for the Encapsulation of Enzymes over Porous Solids for Biodiesel Production and Cellulose Valorization into Glucose

An overview of biomass conversion: exploring new opportunities

Microbial cellulases immobilized in biopolymer/silica matrices used as enzyme release systems

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