“…In 1942 (Movlaee et al, 2017), Bulger and Johns introduced a method based on the use of uricase enzyme though it is commercially available its application is limited due to the cost of uricase enzyme. Alternative methods such as chemiluminescence (Wu et al, 2006), fluorescence (Erden and Kilic, 2013;Afrasiabi et al, 2016), spectrophotometry (Zhao et al, 2009), HPLC-mass spectrometry (Weiss and Lunte, 2000), ion-chromatography (Revin and John, 2012), high performance liquid chromatography and calorimetry (Zhuang et al, 2017) have been reported. However these methods are usually expensive, laborious and complex to perform.…”