The review presents the most elegant and promising set of synthetic routes for the synthesis of 2‐aminothiophenes by the Gewald reaction. Applications of this facile methodology to pharmaceuticals and dyestuffs have been demonstrated.

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The present review discusses the fluorescent organelle probe, DiOC6(3), with reference to its structure, chemistry, availability, spectral properties, labeling procedures, vital staining characteristics, and major applications in cellular and molecular biology. The specificity of dye for endoplasmic reticulum is summarized. We examine the simplicity and advantages of the fluorescent dye system for evaluating structure and function of endoplasmic reticulum. Other significant uses of the dye are also discussed.

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