Pore size, tissue ingrowth, and endothelialization of small-diameter microporous polyurethane vascular prostheses

Zhang, Ze; Wang, Zhaoxu; Li, Shuqin; Kodama, Makoto

doi:10.1016/s0142-9612(03)00478-2

Cited by 128 publications

(103 citation statements)

References 19 publications

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“…To seed BMCs in ePTFE vascular prostheses stably, high porosity (60 to 90 m and more; fibril length) is thought to be advantageous for endothelialization in vivo, because the anchoring of seeded cells and the capillary ingrowth from perigraft tissue are likely to occur. [11][12][13] Hence, in this study, we chose highporosity ePTFE (90 m; fibril length) as a scaffold and were able to succeed in expressing recombinant proteins. This result may be consistent with the work of Noishiki et al 4 demonstrating that BMC-seeded high-porosity vascular prosthesis exhibited autocrine function of angiogenic factor.…”

Section: Discussionmentioning

confidence: 99%

Synthetic Vascular Prosthesis Impregnated With Mesenchymal Stem Cells Overexpressing Endothelial Nitric Oxide Synthase

et al. 2006

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Background-Endothelial dysfunction is known to exaggerate coronary artery disease, sometimes leading to irreversible myocardial damage. In such cases, repetitive coronary revascularization including coronary artery bypass grafting is needed, which may cause a shortage of graft conduits. On the other hand, endothelial nitric oxide synthase (eNOS) is an attractive target of cardiovascular gene therapy. The vascular prostheses, of which the inner surfaces are covered with mesenchymal stem cells (MSCs) overexpressing eNOS, are expected to offer feasible effects of NO and angiogenic effects of MSCs on the native coronary arterial beds, as well as improvement of self-patency. Herein, we attempted to develop small caliber vascular prostheses generating the bioactive proteins. Also, we attempted to transduce eNOS cDNA into MSCs. Methods and Results-The MSCs were isolated from rat bone marrow and transduced with each adenovirus harboring rat eNOS cDNA and ␤-galactosidase (␤-gal) (eNOS/MSCs and ␤-gal/MSCs). The ␤-gal/MSCs were impregnated into vascular prostheses, then the expressions of ␤-gal on the inner surfaces of them were evaluated by 5-bromo-4-chloro-3-indolyl ␤-D-galactoside staining. The NOS activity of eNOS/MSCs was assayed by monitoring the conversion of 3 H-arginine to 3 H-citrulline. The inner surfaces of the vascular prostheses were covered with MSCs expressing ␤-gal. The amount of the 3 H-citrulline increased, and eNOS/MSCs were determined to generate enzymatic activity of eNOS. This activity was completely inhibited by N G -nitro-L-arginine methyl ester. Key Words: mesenchymal stem cells Ⅲ endothelial nitric oxide synthase Ⅲ gene-transduction Ⅲ adenovirus Ⅲ small caliber expanded polytetrafluoroethylene (ePTFE) vascular prosthesis I mpaired endothelial function induces several cardiovascular diseases, including atherosclerosis, hypertension, heart failure, arteriosclerosis obliterans, and coronary heart disease. Endothelial dysfunction also causes NO insufficiency, resulting in the limitation of NO-mediated signal transduction and excretion of bioactive hormone-like products. 1 The attenuated production of NO and the hormone-like products exaggerates these cardiovascular diseases further. Coronary artery disease is one of the most critical diseases enhanced by endothelial dysfunction, sometimes leading to irreversible myocardial damage. Some patients require repetitive coronary revascularization, including coronary artery bypass grafting, which may lead to a shortage of graft materials. Conclusions-TheSmall caliber vascular prostheses, which would be suitable for graft conduits for coronary artery bypass grafting or arteriosclerosis obliterans below the knee, have an extremely high failure rate that is attributed to thrombus formation and occlusion. Many attempts have been made to increase the patency of small-caliber vascular prostheses over the years. Recently, vascular grafts releasing NO infer possibilities preventing thrombosis and stenosis. 2,3 However, they are far from practical use, because the ma...

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Section: Discussionmentioning

confidence: 99%

Synthetic Vascular Prosthesis Impregnated With Mesenchymal Stem Cells Overexpressing Endothelial Nitric Oxide Synthase

et al. 2006

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“…Thus special attention needs to be paid to the cell-biomaterials interactions which control cell proliferation and phenotype. [5][6][7][8] Attempts have been made to minimize the risk of thrombotic complications and to enhance endothelialization, for example, by the introduction of micropores into the conduit wall. [9][10][11] Furthermore, long term patency, integration into the surrounding tissue, and also inflammation are determined not only by the material but also considerably by the production method.…”

Section: Introductionmentioning

confidence: 99%

“…[14][15][16] Polyurethanes are considered promising base materials for the fabrication of vascular grafts because of their superior tensile and elastic properties and their known biocompatibility. 6,12,17,18 Therefore, the electrospinning technique was chosen to process polyetherurethane for the production of vascular grafts. Spinning parameters were selected to implement a reproducible fabrication process which was investigated by measuring the mechanical strength of the constructs.…”

Section: Introductionmentioning

confidence: 99%

Electrospun polyurethane vascular grafts: In vitro mechanical behavior and endothelial adhesion molecule expression

Grasl

Bergmeister

Stoiber

et al. 2009

J Biomedical Materials Res

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Engineered small diameter vascular grafts must closely match mechanical characteristics of native vessels and exhibit stimulus-responsive bioactivity. In this study, mechanical homogeneity of electrospun small diameter polyurethane grafts as well as spontaneous attachment, proliferation, and adhesion molecule expression of endothelial cells (EC) in their presence was studied in vitro. Axial and circumferential tensile strengths were measured and found to be twofold higher in the circumferential direction. EC attachment was easily achieved without precoating the fiber matrix. Stimulation of EC with interleukin-1beta (IL-1beta) led to a statistically significant upregulation of the adhesion molecules E-Selectin, ICAM-1, and VCAM-1. Quantification of adhesion molecule expression by means of energy-dispersive X-ray microanalysis revealed no differences in the stimulatory responses of EC cultured on electrospun polyurethane when compared with cells grown on tissue culture-treated cover slips. Summarizing, highly uniform small diameter polyurethane grafts were fabricated and shown to allow spontaneous EC attachment. The synthetic graft surface neither impaired the endothelial response toward IL-1beta stimulation nor did it adversely affect the regulation of expression of endothelial adhesion molecules.

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“…They found the highest rate of endothelialization with grafts of pore size 30 mm in the abdominal aortas of rats. 115 It was thought that ingrowth of perigraft collagenous tissues aided in the establishment of the endothelium by presenting an ECM suitable for establishment of an endothelial layer. The most significant impact of porosity on the rate of endothelialization may be the establishment of these subendothelium tissues that are crucial to native tissue replacement of a biodegradable small-diameter graft.…”

mentioning

confidence: 99%

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Melchiorri

Hibino

Fisher

2013

Tissue Engineering Part B: Reviews

154

147

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Pore size, tissue ingrowth, and endothelialization of small-diameter microporous polyurethane vascular prostheses

Cited by 128 publications

References 19 publications

Synthetic Vascular Prosthesis Impregnated With Mesenchymal Stem Cells Overexpressing Endothelial Nitric Oxide Synthase

Synthetic Vascular Prosthesis Impregnated With Mesenchymal Stem Cells Overexpressing Endothelial Nitric Oxide Synthase

Electrospun polyurethane vascular grafts: In vitro mechanical behavior and endothelial adhesion molecule expression

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

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