Pore Size of 3D-Printed Polycaprolactone/Polyethylene Glycol/Hydroxyapatite Scaffolds Affects Bone Regeneration by Modulating Macrophage Polarization and the Foreign Body Response

Li, Wenfeng; Dai, Fang; Zhang, Shan; Xu, Fancheng; Xu, Zhiyong; Liao, Shousheng; Zeng, Liangtao; Li, Song; Ai, Fanrong

doi:10.1021/acsami.2c02001

Cited by 33 publications

(28 citation statements)

References 47 publications

(83 reference statements)

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“…Similarly, Wang et al (2014) reported that 30 μm pore size was beneficial to the phenotypic differentiation of M2 macrophages. However, in the study by Li and co-workers ( Li et al, 2022 ), scaffolds with the pore size of 600 μm had better M2 cell infiltration. These differences may be due to the different raw materials and fabrication processes of the scaffolds.…”

Section: Biomaterials Design and Macrophage Regulationmentioning

confidence: 88%

Biomaterial scaffolds regulate macrophage activity to accelerate bone regeneration

Liu

Zhu²,

Li³

et al. 2023

Front. Bioeng. Biotechnol.

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Bones are important for maintaining motor function and providing support for internal organs. Bone diseases can impose a heavy burden on individuals and society. Although bone has a certain ability to repair itself, it is often difficult to repair itself alone when faced with critical-sized defects, such as severe trauma, surgery, or tumors. There is still a heavy reliance on metal implants and autologous or allogeneic bone grafts for bone defects that are difficult to self-heal. However, these grafts still have problems that are difficult to circumvent, such as metal implants that may require secondary surgical removal, lack of bone graft donors, and immune rejection. The rapid advance in tissue engineering and a better comprehension of the physiological mechanisms of bone regeneration have led to a new focus on promoting endogenous bone self-regeneration through the use of biomaterials as the medium. Although bone regeneration involves a variety of cells and signaling factors, and these complex signaling pathways and mechanisms of interaction have not been fully understood, macrophages undoubtedly play an essential role in bone regeneration. This review summarizes the design strategies that need to be considered for biomaterials to regulate macrophage function in bone regeneration. Subsequently, this review provides an overview of therapeutic strategies for biomaterials to intervene in all stages of bone regeneration by regulating macrophages.

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Section: Biomaterials Design and Macrophage Regulationmentioning

confidence: 88%

Biomaterial scaffolds regulate macrophage activity to accelerate bone regeneration

Liu

Zhu²,

Li³

et al. 2023

Front. Bioeng. Biotechnol.

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“…Luo et al, concluded that porous scaffolds with a pore size of 400–600 μm better promote osteogenesis and osseointegration [ 51 ]. Although some scholars believe that a small pore size (188 μm) is more favorable for the osteogenic differentiation of cells in vitro [ 52 ], more studies have demonstrated that 400–700 μm is a good choice for the preparation of bone tissue engineering scaffolds [ 49 , 50 , 51 , 53 ]. In this study, the standard pore size of the scaffold was 600 μm, while the printed PF scaffold had a pore size of approximately 564 μm and the pore size of the GPF scaffold was approximately 508 μm.…”

Section: Discussionmentioning

confidence: 99%

3D-Printed GelMA/PEGDA/F127DA Scaffolds for Bone Regeneration

Gao

Jin

et al. 2023

JFB

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Tissue-engineered scaffolds are an effective method for the treatment of bone defects, and their structure and function are essential for bone regeneration. Digital light processing (DLP) printing technology has been widely used in bone tissue engineering (BTE) due to its high printing resolution and gentle printing process. As commonly used bioinks, synthetic polymers such as polyethylene glycol diacrylate (PEGDA) and Pluronic F127 diacrylate (F127DA) have satisfactory printability and mechanical properties but usually lack sufficient adhesion to cells and tissues. Here, a compound BTE scaffold based on PEGDA, F127DA, and gelatin methacrylate (GelMA) was successfully prepared using DLP printing technology. The scaffold not only facilitated the adhesion and proliferation of cells, but also effectively promoted the osteogenic differentiation of mesenchymal stem cells in an osteoinductive environment. Moreover, the bone tissue volume/total tissue volume (BV/TV) of the GelMA/PEGDA/F127DA (GPF) scaffold in vivo was 49.75 ± 8.50%, higher than the value of 37.10 ± 7.27% for the PEGDA/F127DA (PF) scaffold and 20.43 ± 2.08% for the blank group. Therefore, the GPF scaffold prepared using DLP printing technology provides a new approach to the treatment of bone defects.

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“…Studies have shown that pore size and high porosity also affect the polarization of macrophages, regardless of the implant material [ 128 , 158 , 173 ]. Thus, 3D-printed polycaprolactone/polyethylene glycol/ HA scaffolds were, in vitro, tested on RAW 264.7 cells.…”

Section: Prospects For Affecting Immune Response Through Implant Modi...mentioning

confidence: 99%

Interaction of Ceramic Implant Materials with Immune System

Rafikova

Piatnitskaia

Shapovalova

et al. 2023

IJMS

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The immuno-compatibility of implant materials is a key issue for both initial and long-term implant integration. Ceramic implants have several advantages that make them highly promising for long-term medical solutions. These beneficial characteristics include such things as the material availability, possibility to manufacture various shapes and surface structures, osteo-inductivity and osteo-conductivity, low level of corrosion and general biocompatibility. The immuno-compatibility of an implant essentially depends on the interaction with local resident immune cells and, first of all, macrophages. However, in the case of ceramics, these interactions are insufficiently understood and require intensive experimental examinations. Our review summarizes the state of the art in variants of ceramic implants: mechanical properties, different chemical modifications of the basic material, surface structures and modifications, implant shapes and porosity. We collected the available information about the interaction of ceramics with the immune system and highlighted the studies that reported ceramic-specific local or systemic effects on the immune system. We disclosed the gaps in knowledge and outlined the perspectives for the identification to ceramic-specific interactions with the immune system using advanced quantitative technologies. We discussed the approaches for ceramic implant modification and pointed out the need for data integration using mathematic modelling of the multiple ceramic implant characteristics and their contribution for long-term implant bio- and immuno-compatibility.

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Pore Size of 3D-Printed Polycaprolactone/Polyethylene Glycol/Hydroxyapatite Scaffolds Affects Bone Regeneration by Modulating Macrophage Polarization and the Foreign Body Response

Cited by 33 publications

References 47 publications

Biomaterial scaffolds regulate macrophage activity to accelerate bone regeneration

Biomaterial scaffolds regulate macrophage activity to accelerate bone regeneration

3D-Printed GelMA/PEGDA/F127DA Scaffolds for Bone Regeneration

Interaction of Ceramic Implant Materials with Immune System

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