Porcine wound models for skin substitution and burn treatment

Middelkoop, Esther; Bogaerdt, Antoon J. van den; Lamme, Evert N.; Hoekstra, M.J.; Brandsma, Kor; Ulrich, Magda M. W.

doi:10.1016/s0142-9612(03)00502-7

Cited by 128 publications

(97 citation statements)

References 32 publications

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“…Total number of full-thickness wounds are generally limited to 8 per animal. Wounds of this size would be expected to achieve 40% reduction with granulation in 7 to 8 days (M. M. Swindle 2007; Chvapil 1992; Middelkoop et al 2004). Incisional wounds are made using a scalpel and are also studied for testing new closure devices or suture materials.…”

Section: Excisional and Incisional Wound Healingmentioning

confidence: 99%

The Miniature Swine as a Model in Experimental and Translational Medicine

2016

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The use of the miniature swine as a nonrodent species in research has continued to expand for over a decade, and they are becoming routinely used both in experimental pharmacology and as a therapeutic model for human diseases. Miniature swine models are regularly used for studies designed to assess efficacy and safety of new therapeutic compounds given through different routes of exposure and are used as an alternative model to rodents, canines, or nonhuman primates. Translational preclinical swine study data presented here support the current understanding that miniature swine are the animal model of choice for the assessment of drugs targeting endocrine, dermal, and ocular disorders. Because research investigators need to be familiar with some of the important features of the models developed in the miniature swine in order to place clinical and experimental findings in their proper perspective, relevant references and data from these models will be presented, compared, and partially illustrated.

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Section: Excisional and Incisional Wound Healingmentioning

confidence: 99%

The Miniature Swine as a Model in Experimental and Translational Medicine

2016

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“…Apart from the loss of tissue function, such impairment generates social and psychological tribulations. The reconstruction or replacement of damaged tissues is one of the objectives behind tissue engineering, especially in substantial burns and chronic wounds, because current treatments are insufficient in preventing scar formation and promoting restorative healing [1] . Moreover, tissue loss can be extensive and the healing process may not be sufficient for a theraupetic healing of the tissue.…”

Section: Introductionmentioning

confidence: 99%

Porcine skin as a source of biodegradable matrices: alkaline treatment and glutaraldehyde crosslinking

2010

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Abstract:In this work, the modifications promoted by alkaline hydrolysis and glutaraldehyde (GA) crosslinking on type I collagen found in porcine skin have been studied. Collagen matrices were obtained from the alkaline hydrolysis of porcine skin, with subsequent GA crosslinking in different concentrations and reaction times. The elastin content determination showed that independent of the treatment, elastin was present in the matrices. Results obtained from in vitro trypsin degradation indicated that with the increase of GA concentration and reaction time, the degradation rate decreased. From thermogravimetry and differential scanning calorimetry analysis it can be observed that the collagen in the matrices becomes more resistant to thermal degradation as a consequence of the increasing crosslink degree. Scanning electron microscopy analysis indicated that after the GA crosslinking, collagen fibers become more organized and well-defined. Therefore, the preparations of porcine skin matrices with different degradation rates, which can be used in soft tissue reconstruction, are viable.

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“…Fibroblasts, being present in great amounts on the skin, were chosen to conduct the test. They are responsible for skin cell regeneration and are used for testing biomaterials for skin substitutes [11,33,41,42]. To monitor cell adhesion, the biomaterials were stained with haematoxylin-eosin and imaged with a Leica microscope and micrometrics camera.…”

Section: Cell Adhesionmentioning

confidence: 99%

Bioactive 3D-Shaped Wound Dressings Synthesized from Bacterial Cellulose: Effect on Cell Adhesion of Polyvinyl Alcohol Integrated In Situ

Osorio

Velásquez-Cock

Múnera

et al. 2017

International Journal of Polymer Science

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We investigated wound dressing composites comprising fibrils of bacterial cellulose (BC) grown by fermentation in the presence of polyvinyl alcohol (PVA) followed by physical crosslinking. The reference biointerface, neat BC, favoured adhesion of fibroblasts owing to size exclusion effects. Furthermore, it resisted migration across the biomaterial. Such effects were minimized in the case of PVA/BC membranes. Therefore, the latter are suggested in cases where cell adhesion is to be avoided, for instance, in the design of interactive wound dressings with facile exudate control. The bioactivity and other properties of the membranes were related to their morphology and structure and considered those of collagen fibres. Bioactive materials were produced by simple 3D templating of BC during growth and proposed for burn and skin ulcer treatment.

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Porcine wound models for skin substitution and burn treatment

Cited by 128 publications

References 32 publications

The Miniature Swine as a Model in Experimental and Translational Medicine

The Miniature Swine as a Model in Experimental and Translational Medicine

Porcine skin as a source of biodegradable matrices: alkaline treatment and glutaraldehyde crosslinking

Bioactive 3D-Shaped Wound Dressings Synthesized from Bacterial Cellulose: Effect on Cell Adhesion of Polyvinyl Alcohol Integrated In Situ

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