Porcine teschovirus in wild boars in Hungary

Abstract

The genus Teschovirus, family Picornaviridae, currently includes 12 serotypes (PTV 1 to 12) isolated from swine. PTVs have been well studied in domestic pigs, but knowledge about PTVs in wild boars is deficient. Here, we report the first complete PTV genome sequence from 7 (70%) of 10 fecal samples of wild boar piglets (Sus scrofa) by RT-PCR and pyrosequencing. Analysis of the wild boar PTV strain WB2C-TV/2011/HUN (JQ429405) showed considerable difference, especially in VP1 (66–74% amino acid identity) compared to the available PTVs. PTV is present in wild boars, and WB2C-TV/2011/HUN represents a novel PTV genotype, provisionally named PTV-13.

Two Closely Related Novel Picornaviruses in Cattle and Sheep in Hungary from 2008 to 2009, Proposed as Members of a New Genus in the Family Picornaviridae

“…Both BHuV-1 and OHuV-1 appear to possess a type II IRES, based upon the sequence and secondary-structure similarities to human parechoviruses and Ljungan virus in the genus Parechovirus (9), with high nucleotide similarity in IRES core domains H-I-J-K-L. While teschoviruses, identified only in domestic pigs (1) and wild boar (6), have a type IV IRES (12), the high nucleotide identity of hungarovirus IRESs to the IRES of human parechoviruses, especially to that of HPeV-3, is particularly surprising and interesting. Apart from HPeV-1 and HPeV-6, which have been found in monkeys in China (29), human parechoviruses (or the 5= UTR genome part of parechoviruses) have not yet been reported from nonhuman hosts.…”

Genetic characteristics of the complete feline kobuvirus genome

“…1c) may serve as a core-binding site of p(Y) tract-binding protein (PTB) [17] and suggests that the kobuvirus 3 0 -UTRs may contain specific binding sites for Virus Genes viral or cellular proteins [18]. The ''barbell-like'' structure was also recognizable in members of the Avihepatovirus, Gallivirus, and Kobuvirus genera [19], although the function of this conserved structure is unknown. Comparative sequence analysis revealed that feline strain 12D240 shares nucleotide/amino acid sequence identity with those of cat (94.4/97.4 %), dog (79.5/92.1 %), mouse (75.8/89.4 %), and human strains (74.4/88.7 %).…”

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

“…In addition, Boros et al (2012a) proposed a novel EV-G genotype, provisionally named PEV-15, after characterizing the complete genome sequence of an EV-G strain from a wild boar. This same research group also suggested that there may be PTV strains well-adapted or specific to wild boar host species (Boros et al 2012b). …”

“…The present study detected PTV, PSV, and EV-G more frequently in animals up to 7 months of age relative to adult (2-to 4-year-old) wild boars. Another research group reported high frequencies of positive results for EV-G and PTV infections in 6-and 8-week-old wild boars (Boros et al 2012a, Boros et al 2012b). Due to the lack of data, the results from these studies represent supplementary information that should be used in further age distribution investigations of porcine enteric picornavirus infections in wild boars.…”

“…Wild boars are susceptible to infections by viruses that also affect domestic pigs (Sus scrofa domesticus), such as porcine group A rotavirus (PoRVA), porcine circovirus type 2 (PCV2), hepatitis E virus (HEV), Aujeszky disease virus (ADV), porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSF), torque teno sus virus (TTSuV), influenza A virus, and others (Ruiz-Fons et al 2008, Meng et al 2009, Okadera et al 2013, Biondo et al 2014. These animals are also susceptible to infections by PTV, PSV, and EV-G, which were described in asymptomatic wild boars in Hungary (Boros et al 2012a, Boros et al 2012b, Spain (Cano--Gómez et al 2013), and the Czech Republic (Prodělalová 2012).…”