Genetic characteristics of the complete feline kobuvirus genome

Choi, Jong-Woo; Lee, Myoung-Heon; Lee, Kyoung-Ki; Oem, Jae‐Ku

doi:10.1007/s11262-014-1144-y

Cited by 10 publications

(20 citation statements)

References 25 publications

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“…In this study, we provided the first evidence to support FeKoV circulation in the Chinese feline population, demonstrating that FeKoV is not geographically restricted to Korea and Italy (Cho et al, 2014(Cho et al, , 2015Choi et al, 2015;Chung et al, 2013;Di Martino et al, 2015).…”

Section: Discussionmentioning

confidence: 69%

“…Using reverse transcription polymerase chain reaction (RT-PCR), six of 39 collected faecal samples from Korean cats were confirmed positive for kobuvirus RNA. Phylogenetic analysis revealed that FeKoV (12D240, FK-13) is most closely related to the canine kobuvirus (Choi et al, 2015). In 2015, Barbara Di Martino et al screened faecal samples obtained from asymptomatic and diarrhoeic cats for FeKoV RNA in Italy (Di Martino, Profio, Melegari, Marsilio, & Martella, 2015).…”

Section: )mentioning

confidence: 99%

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First report and genetic characterization of feline kobuvirus in diarrhoeic cats in China

Zhang

Luo

et al. 2018

Transbound Emerg Dis

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Feline kobuvirus (FeKoV) is a newly discovered organism, classified under the species Aichivirus A of the genus Kobuvirus. Since it was first reported in 2013, molecular evidence for FeKoV in the feline population has been restricted to two countries: Korea and Italy. In this study, we collected faecal samples from cats in southern China and detected the FeKoV RNA in these samples. A prevalence rate of 9.9% (8/81) was identified by RT-PCR, and all positive samples were obtained from diarrhoeic animals. In addition, FeKoV was shown positive associated with diarrhoea in cats, with a correlation coefficient of 0.25. Next, we designed three primer pairs with degenerate bases, which targeted the conservative overlapping region of the entire published FeKoV genome, and sequenced the near-complete genome of the first Chinese field FeKoV strain, WHJ-1, using long-fragment PCR. Finally, we analysed WHJ-1's homology and phylogeny using the polyprotein gene. The results indicated that FeKoV has rapidly mutated since it was first discovered. This study will help to better understand FeKoV's epidemiology, evolutionary pattern and genetic diversity.

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Section: Discussionmentioning

confidence: 69%

Section: )mentioning

confidence: 99%

First report and genetic characterization of feline kobuvirus in diarrhoeic cats in China

Zhang

Luo

et al. 2018

Transbound Emerg Dis

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“…Genomic analysis showed that the polyprotein of the two strains were all cleaved into 11 viral proteins, L, VP0, VP3, VP1, 2A, 2B, 2C, 3A, 3B, 3C and 3D. The predicted cleavage sites were Q/G, Q/H, Q/A and Q/S, in accordance with the Korean FeKoV strains, 12D240 and FK-13, and the Chinese strain, WHJ-1 (Cho et al 2014;Choi et al 2015;Lu et al 2018). One of the important finding is that one-amino-acid deletion in VP0 gene of 17CC0811.…”

Section: Tablementioning

confidence: 57%

“…The 28 FeKoV sequences were divided into two major groups: 22 sequences clustered with the Chinese FeKoV strain, WHJ-1 (Lu et al 2018), and formed a major group, while the other 6 sequences and FeKoV reference strains identified in South Korea and Italy formed another group. Interestingly, only one sequence identified in this study appeared more closely related to the Korean FeKoV strain, 12D240 (Choi et al 2015), while other sequences were separated from these reference sequences (Fig. 1).…”

Section: Phylogenetic Analysis Of Partial 3d Genementioning

confidence: 71%

“…FeKoV RNA was found in 8 domestic cats with diarrhea, but was undetected in healthy cats (Lu et al 2018). However, only four complete genomes of FeKoV strains, including FK-13 (Cho et al 2014), 12D240 (Choi et al 2015), TE/52/IT/2013 (Di Martino et al 2015b) and WHJ-1 (Lu et al 2018), have been sequenced until now. Furthermore, there is no data of kobuvirus infections in cats in northeast China.…”

Section: Introductionmentioning

confidence: 99%

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Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China

Niu

Wang

et al. 2019

Infection, Genetics and Evolution

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A B S T R A C TFeline kobuvirus (FeKoV), a novel picornavirus of the genus kobuvirus, was initially identified in the feces of cats with diarrhea in South Korea in 2013. To date, there is only one report of the circulation of kobuvirus in cats in southern China. To investigate the presence and genetic variability of FeKoV in northeast China, 197 fecal samples were collected from 105 cats with obvious diarrhea and 92 asymptomatic cats in Shenyang, Jinzhou, Changchun, Jilin and Harbin regions, Northeast China, and viruses were detected by RT-PCR with universal primers targeting all kobuviruses. Kobuvirus was identified in 28 fecal samples with an overall prevalence of 14.2% (28/197) of which 20 samples were co-infected with feline parvovirus (FPV) and/or feline bocavirus (FBoV). Diarrhoeic cats had a higher kobuvirus prevalence (19.1%, 20/105) than asymptomatic cats (8.7%, 8/ 92). By genetic analysis based on partial 3D gene, all kobuvirus-positive samples were more closely related to previous FeKoV strains with high identities of 90.5%-97.8% and 96.6%-100% at the nucleotide and amino acid levels. Additionally, phylogenetic analysis based on the complete VP1 gene indicated that all FeKoV strains identified in this study were placed into a cluster, which separated from other reference strains previously reported, and three identical amino acid substitutions were present at the C-terminal of the VP1 protein for these FeKoV strains. Furthermore, two complete FeKoV polyprotein genomes were successfully obtained from two positive samples and designated 16JZ0605 and 17CC0811, respectively. The two strains shared 92.9%-94.9% nucleotide identities and 96.8%-98.4% amino acid identities to FeKoV prototype strains. Phylogenetic analysis indicated that FeKoVs were clustered according to their geographical regions, albeit with limited sequences support. This study provides the first molecular evidence that FeKoV circulates in cats in northeast China, and these FeKoVs exhibit genetic diversity and unique evolutionary trend.

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Epidemiology and genetic characteristics of murine kobuvirus from faecal samples of Rattus losea, Rattus tanezumi and Rattus norvegicus in southern China

Zhang

You

et al. 2021

Journal of General Virology

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Recently, murine kobuvirus (MuKV), a novel member of the family Picornaviridae, was identified in faecal samples of Rattus norvegicus in China. The limited information on the circulation of MuKV in other murine rodent species prompted us to investigate its prevalence and conduct a genetic characterization of MuKV in Rattus losea, Rattus tanezumi and Rattus norvegicus in China. Between 2015 and 2017, 243 faecal samples of these three murine rodent species from three regions in southern China were screened for the presence of MuKV. The overall prevalence was 23.0% (56/243). Three complete MuKV polyprotein sequences were acquired, and the genome organization was determined. Phylogenetic analyses suggested that our sequences were closely related to Chinese strains and belong to the species Aichivirus A in the genus Kobuvirus. Additional studies are required to understand the true prevalence of MuKV in murine rodent populations in China.

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Genetic characteristics of the complete feline kobuvirus genome

Cited by 10 publications

References 25 publications

First report and genetic characterization of feline kobuvirus in diarrhoeic cats in China

First report and genetic characterization of feline kobuvirus in diarrhoeic cats in China

Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China

Epidemiology and genetic characteristics of murine kobuvirus from faecal samples of Rattus losea, Rattus tanezumi and Rattus norvegicus in southern China

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