Porcine Rotaviruses: Epidemiology, Immune Responses and Control Strategies

Vlasova, Anastasia N.; Amimo, Joshua O.; Saif, Linda J.

doi:10.3390/v9030048

Cited by 163 publications

(180 citation statements)

References 216 publications

(311 reference statements)

Supporting

Mentioning

167

Contrasting

Unclassified

Order By: Relevance

“…Swine enteric health is continuously challenged by viral infections that cause severe diarrhoea, high mortality in piglets and economic losses. The pathogenesis of TGEV, PEDV and PRV‐A has been extensively characterized (Pensaert & Martelli, ; Vlasova, Amimo, & Saif, ). However, several novel viruses have been found in the faeces of pigs, such as PDCoV, PKV and PSaV.…”

Section: Discussionmentioning

confidence: 99%

Development of a multiplex RT‐PCR for the detection of major diarrhoeal viruses in pig herds in China

Ding

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

The major enteric RNA viruses in pigs include porcine epidemic diarrhoea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus A (PRV‐A), porcine kobuvirus (PKV), porcine sapovirus (PSaV) and porcine deltacoronavirus (PDCoV). For differential diagnosis, a multiplex RT‐PCR method was established on the basis of the N genes of TGEV, PEDV and PDCoV, the VP7 gene of PRV‐A, and the polyprotein genes of PKV and PSaV. This multiplex RT‐PCR could specifically detect TGEV, PEDV, PDCoV, PRV‐A, PKV and PSaV without cross‐reaction to any other major viruses circulating in Chinese pig farms. The limit of detection of this method was as low as 100–101 ng cDNA of each virus. A total of 398 swine faecal samples collected from nine provinces of China between October 2015 and April 2017 were analysed by this established multiplex RT‐PCR. The results demonstrated that PDCoV (144/398), PSaV (114/398), PEDV (78/398) and PRV‐A (70/398) were the main pathogens, but TGEV was not found in the pig herds in China. In addition, dual infections, for example, PDCoV + PSaV, PDCoV + PRV‐A, PRA‐V + PSaV and PEDV + PDCoV, and triple infections, for example, PDCoV + PRV‐A + PSaV and PEDV + PDCoV + PKV, were found among the collected samples. The multiplex RT‐PCR provided a valuable tool for the differential diagnosis of swine enteric viruses circulating in Chinese pig farms and will facilitate the prevention and control of swine diarrhoea in China.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of a multiplex RT‐PCR for the detection of major diarrhoeal viruses in pig herds in China

Ding

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…Rotaviruses (RVs) are a major cause of intestinal disease in animals, including weaned piglets and young children (Vlasova, Amimo, & Saif, ). RVs form a genus of the Reoviridae family and contain a genome consisting of 11 double‐stranded RNA segments.…”

Section: Introductionmentioning

confidence: 99%

“…Continuous RV classification is being performed by the Rega Instituut, KU Leuven (Belgium) (https://rega.kuleuven.be/cev/viralmetagenomics/virus-classification/7th-RCWG-meeting). The most common group infecting humans and animals is RVA, which causes acute dehydrating diarrhoea (Vlasova et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…To date, 32 G, 47 P, 24 I, 18 R, 17 C, 17 M, 28 A, 18 N, 19 T, 24 E and 19 H genotypes have been identified among RVAs from humans and animals (Esona et al., ), as described at the 7th RCWG meeting (https://rega.kuleuven.be/cev/viralmetagenomics/virus-classification/7th-RCWG-meeting) at Rega Instituut, KU Leuven (Belgium). In pigs, 12 G genotypes (G1 to G6, G8 to G12 and G26) and 16 P genotypes (P[1] to P[8], P[13], P[19], P[23], P[26], P[27], P[28], P[32] and P[34]) of RVA have been identified, and G3, G4, G5, G9 and G11 usually occur in combination with P[5], P[6], P[7], P[13] and P[28], respectively (Vlasova et al., ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A G3P[13] porcine group A rotavirus emerging in China is a reassortant and a natural recombinant in the VP4 gene

Jing

Zhang

Shi

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

Group A rotaviruses (RVAs) are a major cause of serious intestinal disease in piglets. In this study, a novel pig strain was identified in a stool sample from China. The strain was designated RVA/Pig/China/LNCY/2016/G3P[13] and had a G3-P[13]-I5-R1-C1-M1-A8-N1-T1-E1-H1 genome. The viral protein 7 (VP7) and non-structural protein 4 (NSP4) genes of RVA/Pig/China/LNCY/2016/G3P[13] were closely related to cogent genes of human RVAs, suggesting that a reassortment between pig and human strains had occurred. Recombination analysis showed that RVA/Pig/China/LNCY/2016/G3P[13] is a natural recombinant strain between the P[23] and P[7] RVA strains, and crossover points for recombination were found at nucleotides (nt) 456 and 804 of the VP4 gene. Elucidating the biological characteristics of porcine rotavirus (PoRV) will be helpful for further analyses of the epidemic characteristics of this virus. The results of this study provide valuable information for RVA recombination and evolution and will facilitate future investigations into the molecular pathogenesis of RVAs.

show abstract

“…Its infection appears to be clinically milder with lower neonatal mortality rate of 30-40% compared to typical PEDV infection. PoRV is also a common cause of viral diarrhea diseases among newborn piglets [14,15]. Infection with any of these viruses develops into similar clinical symptoms, including severe diarrhea and dehydration, and in some cases, mixed infections of these pathogens are common; therefore, it is difficult to distinguish them clinically [4,[16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Dual priming oligonucleotide (DPO)-based real-time RT-PCR assay for accurate differentiation of four major viruses causing porcine viral diarrhea

Jia

Feng

Wang

et al. 2019

Molecular and Cellular Probes

View full text Add to dashboard Cite

A B S T R A C TCurrently in China, porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus (PoRV), and porcine deltacoronavirus (PDCoV) are the major causes of porcine viral diarrhea, and mixed infections in clinics are common, resulting in significant economic losses in pig industry. Here, a dual priming oligonucleotide (DPO)-based multiplex real-time SYBR Green RT-PCR assay were developed for accurately differentiating PEDV, TGEV, PoRV, and PDCoV in clinical specimens targeting the N gene of TGEV, PEDV, and PDCoV, and the VP7 gene of PoRV. Results showed that the DPO primer allowed a wider annealing temperature range (40-65°C) and had a higher priming specificity compared to conventional primer, in which more than 3 nucleotides in the 3′-or 5′-segment of DPO primer mismatched with DNA template, PCR amplification efficiency would decrease substantially or extension would not proceed. DPO-based multiplex real-time RT-PCR method had analytical detection limit of 8.63 × 10 2 copies/μL, 1.92 × 10 2 copies/μL, 1.74 × 10 2 copies/μL, and 1.76 × 10 2 copies/μL for PEDV, TGEV, PoRV, and PDCoV in clinical specimens, respectively. A total of 672 clinical specimens of piglets with diarrheal symptoms were collected in Northeastern China from 2017 to 2018 followed by analysis using the assay, and epidemiological investigation results showed that PEDV, TGEV, PoRV, and PDCoV prevalence was 19.05%, 5.21%, 4.32%, and 3.87%, respectively. The assay developed in this study showed higher detection accuracy than conventional RT-PCR method, suggesting a useful tool for the accurate differentiation of the four major viruses causing porcine viral diarrhea in practice.

show abstract

Porcine Rotaviruses: Epidemiology, Immune Responses and Control Strategies

Cited by 163 publications

References 216 publications

Development of a multiplex RT‐PCR for the detection of major diarrhoeal viruses in pig herds in China

Development of a multiplex RT‐PCR for the detection of major diarrhoeal viruses in pig herds in China

A G3P[13] porcine group A rotavirus emerging in China is a reassortant and a natural recombinant in the VP4 gene

Dual priming oligonucleotide (DPO)-based real-time RT-PCR assay for accurate differentiation of four major viruses causing porcine viral diarrhea

Contact Info

Product

Resources

About