Porcine Pancreatic Lipase as a Catalyst in Organic Synthesis

Еремеев, Н. Л.; Zaitsev, Sergey Y.-U.

doi:10.2174/1570193x13666160225000520

Cited by 22 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This effect can be explained by the significant difference in the water-soluble parts of two chitosans: chitosan 2 (used for the Group 3 nutrition) has had much more content of the water-soluble part than chitosan 1 (used for the Group 2 nutrition). This tendency was supported by our data [21][22][23][24][25] concerning the regulatory effects of chitosan on the activity of lipolytic enzymes, mainly pancreatic lipase in the gastrointestinal tract.…”

Section: Discussionsupporting

confidence: 76%

“…The following biochemical indicators: the concentration of total protein (biuret method), albumin (colorimetric method), urea (enzymatic colorimetric Berthelot method), creatinine (the kinetic Yaffe method), glucose (enzymatic glucose oxidase method), cholesterol (enzyme-colorimetric method), phospholipids (enzyme-colorimetric method), bilirubin (quantification by Walters and Gerarde method); calcium (O-cresolphthalein complexone method), phosphorus, magnesium and iron (colorimetric method); alanine aminotransferase (ALT) activity (UV-kinetic method), aspartate aminotransferase (AST) activity (UV-kinetic method), alkaline phosphatase activity (kinetic method) were determined [15,[19][20][21][22][23][24][25]. The following ratios and indicators: A/G, Ca/P, ALT/AST and the concentration of globulins were determined by calculation [15,19].…”

Section: Animal Bloodmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Study of the Water-Soluble Antioxidants in Fodder Additives and Sheep Blood Serum by Amperometric and Biochemical Methods

Zaitsev

Savina

Volnin

et al. 2020

Animals

View full text Add to dashboard Cite

The effects of chitosan as feed additive for animals (FAFAs) on various digestive processes are an important to study because of the animal nutrition and production quality, healthcare and farming. The aims of this study were to evaluate the total amount of water-soluble antioxidants (TAWSA) of chitosan and high protein microbiologically synthesized concentrate as FAFAs; to assess the effect of these FAFAs on TAWSA values, parameters of sheep blood serum and rumen content by biochemical, physical and chemical methods. The laboratory studies of TAWSA values of feed components based on chitosan from different manufacturers or/and a high-protein concentrate were implemented. The animal experiments were carried out on six rumen-fistulated ewes (in three rounds of 14 days each, i.e., three groups) to confirm the results of the laboratory studies. The particular differences of the TAWSA of sheep blood by using both FAFAs by amperometric method were determined. A strong negative correlation −0.67 (or −0.86) was observed between TAWSA and the total protein (globulin’s) content in the blood for the Group 3 of animals. A moderate (0.40) or strong (0.73) positive correlation between TAWSA and total protein content in the blood for the Group 2 of animals than weak correlation 0.23 (or 0.26) for the control Group 1. In conclusion, the correlations between the value changes of TAWSA vs. major biochemical parameters of a blood serum of rumen-fistulated ewes (Group 3 > Group 2 > Group 1) or some indicators of the rumen content (ingesta pH, total content of volatile fatty acids, etc.) were found for the first time.

show abstract

Section: Discussionsupporting

confidence: 76%

Section: Animal Bloodmentioning

confidence: 99%

Comparative Study of the Water-Soluble Antioxidants in Fodder Additives and Sheep Blood Serum by Amperometric and Biochemical Methods

Zaitsev

Savina

Volnin

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…The biocatalytic promiscuity of enzymes has gained great attention recently and an attempt made to expand their application in organic synthesis. Since catalytic promiscuity could immensely broaden the applicability of enzymatic reactions, extensive investigations have been conducted mainly using hydrolases. − ILs are highly polar liquids, unique chemical reactions have thus been developed using them as a key solvent. − Therefore, it is expected that ILs may influence the promiscuity of enzymatic reaction. I will show such examples in this section.…”

Section: Biocatalysis In Ionic Liquidsmentioning

confidence: 99%

Ionic Liquids as Tool to Improve Enzymatic Organic Synthesis

2017

View full text Add to dashboard Cite

Ionic liquids (ILs) have now been acknowledged as reaction media for biotransformations. The first three examples were reported in this field in 2000, and since then, numerous applications have been reported for biocatalytic reactions using ILs. Two topics using ILs for enzymatic reactions have been reviewed from the standpoint of biocatalyst mediating organic synthesis; the first is "Biocatalysis in Ionic Liquids" which includes various types of biocatalytic reactions in ILs (section 2): (1) recent examples of lipase-mediated reactions using ILs as reaction media for biodiesel oil production and for sugar ester production, (2) oxidase-catalyzed reactions in ILs, (3) laccase-catalyzed reactions, (4) peroxidase-catalyzed reactions, (4) cytochrome-mediated reactions, (5) microbe-mediated hydrations, (6) protease-catalyzed reactions, (8) whole cell mediated asymmetric reduction of ketones, (10) acylase-catalyzed reactions, (11) glycosylation or cellulase-mediated hydrolysis of polysaccharides, (12) hydroxynitrile lyase-catalyzed reaction, (13) fluorinase or haloalkane dehydrogenase-catalyzed reaction, (14) luciferase-catalyzed reactions, and (15) biocatalytic promiscuity of enzymatic reactions for organic synthesis using ILs. The second is "Enzymes Activated by Ionic Liquids for Organic Synthesis", particularly describing the finding story of activation of lipases by the coating with a PEG-substituted IL (section 3). The author's opinion toward "Future Perspectives of Using ILs for Enzymatic Reactions" has also been discussed in section 4.

show abstract

“…Lipases can catalyze a very wide range of reactions, covering not only the formation and hydrolysis of an ester bond, such as esterification or transesterification between an ester and an alcohol or an acid, as well as between two esters, but also amidation, aminolysis, aldol concentration, Michael addition, opening of lactone rings, epoxidation, etc. [ 45 , 145 , 146 , 147 , 148 , 149 , 150 ]. Lipases can exhibit enzymatic activity in various media, including acidic, neutral and alkaline aqueous; aqueous–organic solutions; organic solvents; or ionic liquids, and in the presence of detergents [ 151 ].…”

Section: Enzymes and Polymers Produced With Themmentioning

confidence: 99%

Prospects of Using Biocatalysis for the Synthesis and Modification of Polymers

Nikulin

Švedas

2021

Molecules

View full text Add to dashboard Cite

Trends in the dynamically developing application of biocatalysis for the synthesis and modification of polymers over the past 5 years are considered, with an emphasis on the production of biodegradable, biocompatible and functional polymeric materials oriented to medical applications. The possibilities of using enzymes not only as catalysts for polymerization but also for the preparation of monomers for polymerization or oligomers for block copolymerization are considered. Special attention is paid to the prospects and existing limitations of biocatalytic production of new synthetic biopolymers based on natural compounds and monomers from biomass, which can lead to a huge variety of functional biomaterials. The existing experience and perspectives for the integration of bio- and chemocatalysis in this area are discussed.

show abstract

Porcine Pancreatic Lipase as a Catalyst in Organic Synthesis

Cited by 22 publications

References 0 publications

Comparative Study of the Water-Soluble Antioxidants in Fodder Additives and Sheep Blood Serum by Amperometric and Biochemical Methods

Comparative Study of the Water-Soluble Antioxidants in Fodder Additives and Sheep Blood Serum by Amperometric and Biochemical Methods

Ionic Liquids as Tool to Improve Enzymatic Organic Synthesis

Prospects of Using Biocatalysis for the Synthesis and Modification of Polymers

Contact Info

Product

Resources

About