Porcine Models of Severe Sepsis: Emphasis on Porcine Peritonitis

Goldfarb, Roy D.; Dellinger, R Phillip; Parrillo, Joseph E.

doi:10.1097/01.shk.0000191337.01036.b7

Cited by 32 publications

(36 citation statements)

References 35 publications

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“…As it is known, S aureus releases harmful proteins and enzymes to the cells which cause tissue destruction and severe clinical symptoms (Vehmas-Ali and Sandholm, 1995). In a porcine sepsis model, it was reported that Pseudomonas, S. aureus and E. coli infusions induced acute respiratory failure in sepsis (Goldfarb et al, 2005).…”

Section: Clinical Signs and Bacteriologymentioning

confidence: 99%

The comparative evaluation of serum biochemical, haematological, bacteriological and clinical findings of dead and recovered bitches with pyometra in the postoperative process

Küplülü¹,

Vural²,

Demırel³

et al. 2009

Acta vet. (Beogr.)

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The aim of this study was to investigate the prognosis, the risk status and the death reasons in dead and recovered bitches with pyometra after the operative approach by comparing serum biochemical, and haematological parameters, as well as bacteriological and clinical findings. Bitches with pyometra (n=30) were ovariohysterectomized. Eight bitches that died after the ovariohysterectomy formed Group 1 and the rest, which recovered, was Group 2. In addition, 10 healthy bitches were used as the reference for haematological and biochemical parameters. It was found that the level of band neutrophils in Group 1 was higher than 10% and there was lymphopenia along with a marked monocytosis resulting in a highly negative correlation (r=-0.74). There were significant differences (p<0.01) between Group 1 and 2 for BUN, creatinine and BUN/creatinine ratio. Also, the increased serum BUN and creatinine levels were significantly correlated (rs = 0.605 and rs = 0.514, respectively). A significant tendency was apparent for increasing mortality in bitches with pyometra whose BUN levels were over 30 mg/dL (odds ratio=23.80) and creatinine levels above 1.5 mg/dL (odds ratio=7.518). Clinical signs and bacteriological findings did not lead to marked differences between the groups. In pyometra cases, the elderly azotemic dogs having higher BUN and creatinine concentration, would more likely succumb within 3 days after surgery. It may be concluded that the increased BUN concentrations may serve as a reliable prognostic predictor

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Section: Clinical Signs and Bacteriologymentioning

confidence: 99%

The comparative evaluation of serum biochemical, haematological, bacteriological and clinical findings of dead and recovered bitches with pyometra in the postoperative process

Küplülü¹,

Vural²,

Demırel³

et al. 2009

Acta vet. (Beogr.)

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“…Fourth, the pig is LPS sensitive in contrast to mice but similar to humans (166,167), which is important studying Gram-negative induced inflammation. Fifth, there are high sequence and chromosome structure homology with humans (168), indicating that the majority of in particular the orthologous genes are conserved between the two species.…”

Section: The Pig As a Model Animalmentioning

confidence: 99%

“…IP challenge was therefore not conducted in study V. CLP might be the model that best mimic clinical sepsis (178,179), and is frequently used in small animals. Peritonitis induced sepsis models are also conducted in pigs (167), and although CLP is possible to conduct and has been described in pig models (180), it is rarely used. A CLP model was not possible to use for us, because of limitations of the animal facilities and the possibilities of prolonged surveillance of the animals.…”

Section: Tissue Compartmentalization Is a Well Recognized Feature Of mentioning

confidence: 99%

Candidate inhibitors of porcine complement

Thorgersen¹,

Ghebremariam²,

Thurman³

et al. 2007

Molecular Immunology

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“…In this current feasibility study we assessed whether tIPR could enhance circulation in a well-established model of porcine E. coli peritonitis [19,20]. In this model there is an early hyperdynamic response characterized by increased cardiac output, moderate increase in heart rate and slight changes in peripheral resistances.…”

Section: Introductionmentioning

confidence: 98%

Biphasic intra-thoracic pressure regulation augments cardiac index during porcine peritonitis: a feasibility study

Cinel

Goldfarb

Metzger

et al. 2013

Journal of Medical Engineering & Technology

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Preservation of cardiac output (CO) and pulmonary artery pressure (PAP) is vital to maintaining tissue oxygenation in sepsis. This feasibility study tested the hypothesis that therapeutic intra-thoracic pressure regulation (tIPR), delivered with a novel device, was designed to non-invasively enhance venous return by creating sub-atmospheric intra-thoracic pressure during the expiratory phase of mechanical ventilation, improves CO without fluid resuscitation in a porcine E. coli peritonitis model of sepsis. Seven pigs were intubated, anaesthetized and instrumented with a Swan-Ganz and femoral artery catheter. After a 30 min basal period, a fibrin clot containing 4-5 × 10(9) cfu kg(-1) E. coli O111.B4 was implanted in the peritoneum. One hour after clot implantation, tIPR was utilized for 30 min and then removed from the ventilator circuit for 30 min. This tIPR cycle was repeated 4-times. Changes in haemodynamic parameters were calculated by comparing pre-tIPR values to peak values during tIPR administration. Following peritonitis, tIPR significantly increased the peak cardiac index (mean ± SEM) (14.8 ± 2.6 vs 7.9 ± 2.3 ml kg(-1)) and mean arterial pressure (10.2 ± 1.5 vs 4.9 ± 1.1 mmHg) and simultaneously decreased PAP (-7.7 ± 1.5 vs -2.7 ± 0.8 mmHg). These results support the feasibility of the concept that therapeutic application of negative expiratory pressure may provide a non-invasive and complementary approach to increase cardiac output and organ perfusion in the setting of septic shock.

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Porcine Models of Severe Sepsis: Emphasis on Porcine Peritonitis

Cited by 32 publications

References 35 publications

The comparative evaluation of serum biochemical, haematological, bacteriological and clinical findings of dead and recovered bitches with pyometra in the postoperative process

The comparative evaluation of serum biochemical, haematological, bacteriological and clinical findings of dead and recovered bitches with pyometra in the postoperative process

Candidate inhibitors of porcine complement

Biphasic intra-thoracic pressure regulation augments cardiac index during porcine peritonitis: a feasibility study

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