Porcine epidemic diarrhea virus in Asia: An alarming threat to the global pig industry

Sun, Yankuo; Chen, Yongjie; Han, Xuelei; Yu, Zhiqing; Wei, Yingfang; Zhang, Guihong

doi:10.1016/j.meegid.2019.02.013

Cited by 17 publications

(15 citation statements)

References 9 publications

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“…The differences in genetic component between the current epidemic strains and commercial vaccine strains may be the main reason for this lack of immune protection. Because of the genetic and antigenic diversity of PED isolates, it is necessary to monitor emerging epidemic strains with genomic surveillance programs (Sun et al, 2019). From a molecular epidemiological survey of PEDV done in our lab in 2016, we found a PEDV strain, from one clinical sample of small intestine, that had a specific continuous 36nt deletion in the N gene.…”

Section: Discussionmentioning

confidence: 99%

“…At present, the most prevalent PEDV strains worldwide, especially in China, belong to G2 strains (Lin et al, 2016). G2 strains are further divided into 2 subgroups: G2a mainly occurrs in Asia, while G2b is primarily endemic to Asia and North America (Sun et al, 2019). Liu et al (2019) reported that G2a strain-based inactivated vaccine candidates were more promising than G2b-based candidates for the development of an effective vaccine against the current highly virulent pandemic PEDV strains.…”

Section: Discussionmentioning

confidence: 99%

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Pathogenicity and immunogenicity of a new strain of porcine epidemic diarrhea virus containing a novel deletion in the N gene

Wang

Zhan

et al. 2020

Veterinary Microbiology

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A B S T R A C TSince late 2010, highly virulent PEDV G2-genotype strains have emerged globally extracting heavy losses on the pork industries of numerous countries. We investigated the characteristics of a field strain of PEDV (PEDV strain SH) isolated from a piglet with severe diarrhea on a farm in Shanghai China. Whole genome sequencing and analysis revealed that the SH strain belonged to subtype G2b and has a unique 12-aa deletion (aa 399-410) including the antigenic epitope NEP-1C9 (aa 398-406) of the N protein. PEDV SH strain is highly pathogenic to challenged newborn piglets, resulting in 100 % morbidity and mortality. Pathological examination revealed significant villus atrophy in the jejuna of infected piglets. Mice inoculated with inactivated PEDV SH produced antibodies against the N protein, but no antibodies against the deletions. These results illustrated that deletion of the NEP-1C9 epitope had no effect on the immunogenicity or pathogenicity of PEDV, providing evidence of the necessity to monitor the genetic diversity of the virus. Our study also contributes to development of candidate for vaccines and diagnostics that could differentiate pigs seropositive due to vaccination by conventional strains from wild virus infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pathogenicity and immunogenicity of a new strain of porcine epidemic diarrhea virus containing a novel deletion in the N gene

Wang

Zhan

et al. 2020

Veterinary Microbiology

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“…The global PEDV strains are classified into the classic GI group and variant GII group [4,6]. The PEDV strains in the variant GII group are closely related to the frequent occurrence of porcine epidemic diarrhea, which has resulted in huge economic losses to the pork industry worldwide [9,13]. The PEDV strains in the variant GII group appear to be genetically diverse with a gradual increase in number reported globally.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China

Yang

et al. 2020

Microbial Pathogenesis

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“…In these outbreaks, inactivated vaccines and attenuated live vaccines, which were derived from CV777, were used to control the disease but neither of them provided effective protection ( Sun et al, 2012 ; Zhou et al, 2012 ). Moreover, the virus has evolved since 2010 ( Guo et al, 2019 ; Hsu et al, 2018 ; Sun et al, 2019 ), and acquisition of whole genome features of PEDV provides a convenient tool for the tracking of PEDV epidemiology ( Chen et al, 2019b ). In addition, virus isolation and genetic analysis allow investigations on the prevalence of epidemic strains and will provide information for diagnosis and vaccine developments ( Li et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia

Liang

Zhou

Geng

et al. 2020

PeerJ

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Porcine epidemic diarrhea virus (PEDV) is a leading cause of diarrhea in pigs worldwide. Virus isolation and genetic evolutionary analysis allow investigations into the prevalence of epidemic strains and provide data for the clinical diagnosis and vaccine development. In this study, we investigated the genetic characteristics of PEDV circulation in Asia through virus isolation and comparative genomics analysis. APEDV strain designated HB2018 was isolated from a pig in a farm experiencing a diarrhea outbreak. The complete genome sequence of HB2018 was 28,138 bp in length. Phylogenetic analysis of HB2018 and 207 PEDVs in Asia showed that most PEDV strains circulating in Asia after 2010 belong to genotype GII, particularly GII-a. The PEDV vaccine strain CV777 belonged to GI, and thus, unmatched genotypes between CV777 and GII-a variants might partially explain incomplete protection by the CV777-derived vaccine against PEDV variants in China. In addition, we found the S protein of variant strains contained numerous mutations compared to the S protein of CV777, and these mutations occurred in the N-terminal domain of the S protein. These mutations may influence the antigenicity, pathogenicity, and neutralization properties of the variant strains.

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Porcine epidemic diarrhea virus in Asia: An alarming threat to the global pig industry

Cited by 17 publications

References 9 publications

Pathogenicity and immunogenicity of a new strain of porcine epidemic diarrhea virus containing a novel deletion in the N gene

Pathogenicity and immunogenicity of a new strain of porcine epidemic diarrhea virus containing a novel deletion in the N gene

Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China

Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia

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