Porcine Deltacoronaviruses: Origin, Evolution, Cross-Species Transmission and Zoonotic Potential

Kong, Fanzhi; Wang, Qiuhong; Kenney, Scott P.; Jung, Kwonil; Vlasova, Anastasia N.; Saif, Linda J.

doi:10.3390/pathogens11010079

Cited by 29 publications

(38 citation statements)

References 114 publications

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“…Empirical evidence shows that PDCoV has cross-species transmission ability, zoonotic potential, and a wide host range. It is uncertain whether there exist other unidentified PDCoV hosts or whether the virus will infect new hosts in the future [ 8 , 13 ]. To date, no effective therapeutic agent or commercial vaccine is available for the treatment or prevention of PDCoV infection [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…Subsequent challenge experiments confirmed that PDCoV is an important causative agent of diarrhoea in pigs and particularly in neonates [ 6 , 7 ]. Outbreaks of pig diarrhoea caused by PDCoV were then reported in Canada, Korea, China, Japan, Vietnam, Thailand, Laos, Mexico, and Haiti [ 1 , 8 , 9 ] and caused considerable economic losses to the global pig industry [ 2 ]. Birds, chickens, turkey poults, and calves could also become PDCoV hosts under natural and experimental conditions [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…PDCoV can infect a wide range of cell lines derived from pigs, humans and chickens [ 12 ]. The foregoing evidence suggests that PDCoV poses the potential risk of cross-species transmission [ 8 , 12 , 13 ]. A 2021 study reported that PDCoV was successfully isolated from the plasma of three Haitian children presenting with an acute undifferentiated febrile illness [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…It may occur as a single infection, co-infection, or multiple infections [ 8 , 14 ]. Co-infection of PDCoV with one or two other swine viral enteropathogens such as porcine epidemic diarrhoea virus (PEDV), porcine enteric alphacoronavirus (PEAV), transmissible gastroenteritis virus (TGEV), and porcine rotavirus (PRoV) is fairly common in pig herds worldwide and significantly aggravates existing diarrhoea [ 1 , 8 , 15 ]. The clinical signs and pathological features of the foregoing swine diarrhoeal diseases are often indistinguishable [ 16–18 ].…”

Section: Introductionmentioning

confidence: 99%

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A nucleic acid detection assay combining reverse transcription recombinase-aided amplification with a lateral flow dipstick for the rapid visual detection of porcine deltacoronavirus

et al. 2022

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Porcine deltacoronavirus (PDCoV) is an emerging enteropathogen causing severe diarrhoea, dehydration, and death in nursing piglets and enormous economic losses for the global swine industry. Furthermore, it can infect multiple animal species including humans. Therefore, a rapid, definitive diagnostic assay is required for the effective control of this zoonotic pathogen. To identify PDCoV, we developed a nucleic acid detection assay combining reverse transcription recombinase-aided amplification (RT-RAA) with a lateral flow dipstick (LFD) targeting the highly conserved genomic region in the ORF1b gene. The RT-RAA-LFD assay exhibited good PDCoV detection reproducibility and repeatability and could be completed within 11 min. Ten minutes at 40 °C was required for nucleic acid amplification and 1 min at room temperature was needed for the visual LFD readout. The assay specifically detected PDCoV and did not cross-react with any other major swine pathogens. The 95% limit of detection (LOD) was 3.97 median tissue culture infectious dose PDCoV RNA per reaction. This performance was comparable to that of a reference TaqMan-based real-time RT-PCR (trRT-PCR) assay for PDCoV. Of 149 swine small intestine, rectal swab, and serum samples, 71 and 75 tested positive for PDCoV according to RT-RAA-LFD and trRT-PCR, respectively. The diagnostic coincidence rate for both assays was 97.32% (145/149) and the kappa value was 0.946 (p < 0.001). Overall, the RT-RAA-LFD assay is a user-friendly diagnostic tool that can rapidly and visually detect PDCoV.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A nucleic acid detection assay combining reverse transcription recombinase-aided amplification with a lateral flow dipstick for the rapid visual detection of porcine deltacoronavirus

et al. 2022

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“…Deltacoronavirus/δ-CoV (Daha yakın zamanda tanımlanan cins) içerisinde de domuzlardan (Porcine Deltacoronavirus/PDCoV) ve yabani kuşların yanı sıra, Asya leopar kedisinden izole edilen çeşitli virüsler yer almaktadır [10,11]. Lednicky olacak zoonoz bir özellik kazanma potansiyeli olduklarını vurgulamışlardır [11,12].…”

Section: Betacoronavirus Grup D (Nobecovirus Alt Cinsi) Yalnızca Yara...unclassified

Covid-19 Yayilmasinda Arkadaş Hayvanlarin Rolü

Ünal¹

2022

Ankara Universitesi Eczacilik Fakultesi Dergisi

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Amaç: Yirmi birinci yüzyılda, sağlık alanındaki aşı ve terapötik ajanlarla ilgili gelişmeler ve enfeksiyon yönetimindeki iyileştirmelere rağmen hala insan ve hayvanlarda ölümcül olabilen yeni viral salgınlar ortaya çıkmaktadır. Yarasa kökenli olan ve ara konağı hala tartışılan, zoonoz COVID-19 enfeksiyonu Aralık 2019 yılında Çin'in Wuhan eyaletinde bir hayvan pazarı ziyaret geçmişi olan insanlarda ortaya çıkmıştır. Dünya Sağlık Örgütü, Şubat 2020 tarihinde hastalığın pandemik bir enfeksiyon olduğunu ilan etmiştir. Milyonlarca insan bu salgından etkilenmiştir. Salgın mücadelesinin ekonomik yönden büyük maliyeti olmuş ve olmaya da devam etmektedir. İnsanlar yaşam rutinlerini değiştirmiştir. Bu süreçte arkadaş (companion) hayvanların insanlarla yakın ilişkisi dolayısıyla COVID-19 hastalığına yakalanması, etkeni taşıması, tekrar insanlara bulaştırması ile ilgili kaygılar yaşanmaktadır. Bu derlemenin amacı kedi, köpek, hamster ve atlar gibi arkadaş hayvanlarda COVID-19 varlığı ve bulaşı ile ilgili çalışmaların incelenmesidir.Sonuç ve Tartışma: Yapılan çalışmalarda arkadaş hayvanların çoğunun (kedi, köpek ve hamster) SARS-CoV-2'ye duyarlı olduğu ve insanların onlar için bir enfeksiyon kaynağı olabileceği bildirilmiştir. Ancak arkadaş hayvanların insanlara hastalık bulaştırmasındaki potansiyel rolü tam olarak bilinmemektedir. Bu son salgında, enfeksiyöz etkenlerin özellikle zoonoz olanlarının tek sağlık konseptinde epidemiyolojik olarak araştırılmasının gerekliliği bir kez daha ortaya çıkmıştır.

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Susceptibility to mice and potential evolutionary characteristics of porcine deltacoronavirus

Zhang

Ding

Yuan

et al. 2022

Journal of Medical Virology

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Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes diarrhea in suckling piglets and has the potential for cross‐species transmission, posing a threat to animal and human health. However, the susceptibility profile of different species of mice to PDCoV infection and its evolutionary characteristics are still unclear. In the current study, we found that BALB/c and Kunming mice are susceptible to PDCoV. Our results showed that there were obvious lesions in intestinal and lung tissues from the infected mice. PDCoV RNAs were detected in the lung, kidney, and intestinal tissues from the infected mice of both strains, and there existed wider tissue tropism in the PDCoV‐infected BALB/c mice. The RNA and protein levels of aminopeptidase N from mice were relatively high in the kidney and intestinal tissues and obviously increased after PDCoV infection. The viral‐specific IgG and neutralizing antibodies against PDCoV were detected in the serum of infected mice. An interesting finding was that two key amino acid mutations, D138H and Q641K, in the S protein were identified in the PDCoV‐infected mice. The essential roles of these two mutations for PDCoV‐adaptive evolution were confirmed by cryo‐electron microscope structure model analysis. The evolutionary characteristics of PDCoV among Deltacoronaviruses (δ‐CoVs) were further analyzed. δ‐CoVs from multiple mammals are closely related based on the phylogenetic analysis. The codon usage analysis demonstrated that similar codon usage patterns were used by most of the mammalian δ‐CoVs at the global codon, synonymous codon, and amino acid usage levels. These results may provide more insights into the evolution, host ranges, and cross‐species potential of PDCoV.

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Porcine Deltacoronaviruses: Origin, Evolution, Cross-Species Transmission and Zoonotic Potential

Cited by 29 publications

References 114 publications

A nucleic acid detection assay combining reverse transcription recombinase-aided amplification with a lateral flow dipstick for the rapid visual detection of porcine deltacoronavirus

A nucleic acid detection assay combining reverse transcription recombinase-aided amplification with a lateral flow dipstick for the rapid visual detection of porcine deltacoronavirus

Covid-19 Yayilmasinda Arkadaş Hayvanlarin Rolü

Susceptibility to mice and potential evolutionary characteristics of porcine deltacoronavirus

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