Porcine deltacoronavirus: histological lesions and genetic characterization

Wang, Leyi; Hayes, Jeff; Sarver, C. F.; Byrum, Beverly; Zhang, Yan

doi:10.1007/s00705-015-2627-4

Cited by 41 publications

(49 citation statements)

References 13 publications

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“…In agreement with previous studies (Marthaler, Raymond, et al., ; Wang, Hayes, Sarver, Byrum, & Zhang, ), phylogenetic analysis based on the PDCoV complete genomes exhibited a clear separation between US and Chinese strains. Of which, all Korean PDCoV strains identified in 2014 (KNU14‐04) and in 2016 (KNU16‐07 and KNU16‐11) were grouped within the US PDCoV clade; however, they were situated in different branches (Figure b).…”

Section: Resultssupporting

confidence: 91%

Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014-2016

Jang

Lee

Kim

et al. 2017

Transbound Emerg Dis

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Porcine deltacoronavirus (PDCoV) is a newly emerged enterotropic swine coronavirus that causes enteritis and diarrhoea in piglets. Here, a nested reverse transcription (RT)-PCR approach for the detection of PDCoV was developed to identify and characterize aetiologic agent(s) associated with diarrhoeal diseases in piglets in South Korea. A PCR-based method was applied to investigate the presence of PDCoV in 683 diarrhoeic samples collected from 449 commercial pig farms in South Korea from January 2014 to December 2016. The molecular-based survey indicated a relatively high prevalence of PDCoV (19.03%) in South Korea. Among those, the monoinfection of PDCoV (9.66%) and co-infection of PDCoV (6.30%) with porcine epidemic diarrhoea (PEDV) were predominant in diarrhoeal samples. The full-length genomes or the complete spike genes of the most recent strains identified in 2016 (KNU16-07, KNU16-08 and KNU16-11) were sequenced and analysed to characterize PDCoV currently prevalent in South Korea. We found a single insertion-deletion signature and dozens of genetic changes in the spike (S) genes of the KNU16 isolates. Phylogenetic analysis based on the entire genome and spike protein sequences of these strains indicated that they are most closely related to other Korean isolates grouped with the US strains. However, Korean PDCoV strains formed different branches within the same cluster, implying continuous evolution in the field. Our data will advance the understanding of the molecular epidemiology and evolutionary characteristics of PDCoV circulating in South Korea.

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Section: Resultssupporting

confidence: 91%

Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014-2016

Jang

Lee

Kim

et al. 2017

Transbound Emerg Dis

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“…A retrospective epidemiological study showed that PDCoV was detected in pig samples as early as August 2013 in the United States (McCluskey et al, 2015) and in pig samples in South Korea in May 2014 . Some groups have recently reported the emergence of PDCoV in different areas of the Chinese mainland, and interestingly, we detected PDCoV in pig diarrhea samples collected as early as 2004 (Chen et al, 2015a(Chen et al, , 2015bDong et al, 2015;Song et al, 2015;Wang et al, 2015aWang et al, , 2015b. With increased concern about this newly emerging enteric disease, reverse-transcription polymerase chain reaction (RT-PCR), real-time RT-PCR, nested RT-PCR, and singleplex RT insulated isothermal PCR methods have been developed to detect PDCoV in clinical samples Wang et al, 2014a;Song et al, 2015;Zhang et al, 2015Zhang et al, , 2016.…”

Section: Introductionmentioning

confidence: 89%

Isolation, genomic characterization, and pathogenicity of a Chinese porcine deltacoronavirus strain CHN-HN-2014

Dong

Fang

Yang

et al. 2016

Veterinary Microbiology

111

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Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus that causes diarrhea in piglets. Since the first outbreak of PDCoV in the United States in 2014, this novel porcine coronavirus has been detected in South Korea, Canada, Mexico, Thailand, and China. In this study, a Chinese PDCoV strain, designated CHN-HN-2014, was isolated from piglets with severe diarrhea on a pig farm in Henan Province, China, and examined with a specific immunofluorescence assay and electron microscopy. Genomic analysis showed that CHN-HN-2014 shares 91.6%-99.4% nucleotide identity with other known PDCoV strains. The pathogenicity of CHN-HN-2014 was further investigated in 5-day-old and 21-day-old piglets. Both kinds of piglets developed clear clinical symptoms, including vomiting, anorexia, lethargy, and severe diarrhea, by 2days postinoculation (DPI), and diarrhea persisted for about 5-6 days. Viral shedding was detected in rectal swabs until 14 DPI in challenged 5-day-old pigs and until 18 DPI in challenged 21-day-old pigs. At necropsy at 4 DPI, macroscopic and microscopic lesions were observed and viral antigen was detected in the small intestines with immunohistochemical staining. These data demonstrate that Chinese PDCoV strain CHN-HN-2014 shares high nucleotide identity with previously reported PDCoV strains and is pathogenic in 5-day-old and 21-day-old piglets.

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“…The infection incidents were in wide range within the geographical scope in the affected countries. The piglets naturally infected with PDCoV exhibited diarrhoea, vomiting and dehydration together with histological lesions typical for multifocal to diffuse villous atrophy of the small intestines (Janetanakit et al., ; Song et al., ; Wang, Hayes, Sarver, Byrum, & Zhang, ). The characteristic clinical signs and microscopic intestinal lesions that were associated with PDCoV infection were evidenced by experimental infection studies (Chen, Gauger, et al., ; Jung et al., ).…”

Section: Introductionmentioning

confidence: 99%

Point‐of‐care diagnostic assay for rapid detection of porcine deltacoronavirus using the recombinase polymerase amplification method

Zeng

Huang

et al. 2019

Transbound Emerg Dis

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Summary Porcine deltacoronavirus (PDCoV) has emerged and spread throughout the porcine industry in many countries over the last 6 years. PDCoV caused watery diarrhoea, vomiting and dehydration in newborn piglets. A sensitive diagnostic method would be beneficial to the prevention and control of PDCoV infection. Recombinase polymerase amplification (RPA) is an isothermal amplification method which has been widely used for virus detection. A probe‐based reverse transcription RPA (RT‐RPA) assay was developed for real‐time detection of PDCoV. The amplification can be finished in 20 min and fluorescence monitoring was performed by a portable device. The lowest detection limit of the PDCoV RT‐RPA assay was 100 copies of RNA molecules per reaction; moreover, the RT‐RPA assay had no cross‐reaction with other common swine viruses. The clinical performance of the RT‐RPA assay was evaluated using 108 clinical samples (54 intestine specimens and 54 faecal swab specimens). The coincidence rate of the detection results for clinical samples between RT‐RPA and RT‐qPCR was 97.2%. In summary, the real‐time RT‐RPA assay offers a promising alternative to RT‐qPCR for point‐of‐care detection of PDCoV.

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Porcine deltacoronavirus: histological lesions and genetic characterization

Cited by 41 publications

References 13 publications

Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014-2016

Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014-2016

Isolation, genomic characterization, and pathogenicity of a Chinese porcine deltacoronavirus strain CHN-HN-2014

Point‐of‐care diagnostic assay for rapid detection of porcine deltacoronavirus using the recombinase polymerase amplification method

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