Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014-2016

Jang, Guehwan; Lee, K.‐K.; Kim, S.‐H.; Lee, C.

doi:10.1111/tbed.12690

Cited by 65 publications

(54 citation statements)

References 23 publications

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“…Additionally, all Tibetan pigs infected with PDCoV were under one month of age, therefore, indicating that the prevalence of PDCoV was related to the ages of Tibetan pigs (Table ), consistent with previous studies (Mai et al., ; Song et al., ). Nevertheless, PDCoV‐positive Tibetan pigs showed mild clinical diarrhoeal disease, and no mortality was recorded among the infected pigs with clinical diarrhoeal disease, which is inconsistent with previous reports (Janetanakit et al., ; Jang et al., ). These results suggest that subclinical infection of PDCoV occurs in Tibetan pigs, which are emerging but imperfect hosts for the PDCoV.…”

Section: Discussioncontrasting

confidence: 78%

“…In our present study, we found that the prevalences of PDCoV, PEDV and PDCoV + PEDV in Tibetan pigs were significantly lower than in pigs in other provinces of China (Dong et al., ; Song et al., ), South Korea (Jang, Lee, Kim, & Lee, ), Thailand (Janetanakit et al., ; Saeng‐Chuto et al., ) and the United States(Wang et al., ). The prevalence of PDCoV in Tibetan pigs was relatively low (3.70%), and only piglets were affected.…”

Section: Discussionmentioning

confidence: 44%

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Detection and genetic characterization of porcine deltacoronavirus in Tibetan pigs surrounding the Qinghai-Tibet Plateau of China

Wang

Baloch

et al. 2018

Transbound Emerg Dis

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Porcine deltacoronavirus (PDCoV) is a recently discovered RNA virus that belongs to the family Coronaviridae and genus Deltacoronavirus. This virus causes enteric disease in piglets that is characterized by enteritis and diarrhoea. In our present investigation, 189 diarrhoeic samples were collected between July 2016 and May 2017 from Tibetan pigs inhabiting in three different provinces surrounding the Qinghai-Tibet Plateau of China. We then applied the molecular-based method of reverse transcription polymerase chain reactions (RT-PCRs) to detect the presence of PDCoV in collected samples, and RT-PCR indicated that the prevalence of PDCoV was 3.70% (7/189) in Tibetan pigs. Four of 7 PDCoV-positive pigs were monoinfections of PDCoV, three samples were co-infections of PDCoV with porcine epidemic diarrhoea virus (PEDV), and 52 (27.51%) samples were positive for PEDV. Four strains with different full-length genomes were identified (CHN/GS/2016/1, CHN/GS/2016/2, CHN/GS-/2017/1 and CHN/QH/2017/1), and their genomes were used to analyse the characteristics of PDCoV currently prevalent in Tibetan pigs. We found a 3-nt insertion in the spike gene in four strains in Tibetan pigs. Phylogenetic analysis of the complete genome and spike and nucleocapsid gene sequences revealed that these strains shared ancestors with the strain CHN-AH-2004, which was found in pigs from the Anhui province of China mainland. However, PDCoV strains from Tibetan pigs formed different branches within the same cluster, implying continuous evolution in the field. Our present findings highlight the importance of epidemiologic surveillance to limit the spread of PDCoV in livestock at high altitudes in China.

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Section: Discussioncontrasting

confidence: 78%

Section: Discussionmentioning

confidence: 44%

Detection and genetic characterization of porcine deltacoronavirus in Tibetan pigs surrounding the Qinghai-Tibet Plateau of China

Wang

Baloch

et al. 2018

Transbound Emerg Dis

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“…The two-thirds of PDCoV genome at the 5′ end encodes genome replication proteins (ORF1ab) and one-third at the 3′ end encodes four mainly structural proteins, https://doi.org/10. 1016/j.prevetmed.2019.02.017 Received 8 October 2018 Received in revised form 23 February 2019; Accepted 28 February 2019 T spike glycoprotein (S), envelope protein (E), membrane protein (M), and nucleocapsid protein (N), and three accessory proteins, including nonstructural protein 6 (NS6), NS7, and NS7a (Woo et al, 2012;Jang et al, 2017). The PDCoV S protein is a glycoprotein on the viral surface and plays an essential function in mediating viral entry into host cells by binding with host receptors (Graham and Baric, 2010;Shang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, phylogenetic and evolutionary analysis of porcine deltacoronavirus in Henan province, China

Zhang

Liang

et al. 2019

Preventive Veterinary Medicine

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A B S T R A C TPorcine deltacoronavirus (PDCoV) is a novel porcine enteric coronavirus that causes diarrhea, vomiting and dehydration in piglets. This newly virus has spread rapidly and has caused serious economic losses for pig industry since the outbreak in USA in 2014. In this study, 430 faecal and intestinal samples (143 faecal samples and 287 intestinal samples) were collected from individual pigs with diarrhea and 211 serum samples were also collected from the sows with mild diarrhea in 17 regions in Henan province, China from April 2015 to March 2018. The RT-PCR detection indicated that the infection of PDCoV was high up to 23.49% (101/430), and coinfection with PEDV were common (60.40%, 61/101) in Henan pigs. The prevalence of PDCoV in suckling piglets was the highest (36.43%, 94/258). We also found that PDCoV could be detected in sows faeces and sera while the sows showed mild, self-limited diarrhea in clinic. The complete genomes of 4 PDCoV Henan strains (CH-01, HNZK-02, HNZK-04, HNZK-06) were sequenced and analyzed. Phylogenetic analysis based on the complete genome, spike and nucleocapsid gene sequences revealed that the PDCoV Henan strains were closely related to other PDCoV reference strains that located in the Chinese clade. Furthermore, the phylogenetic analysis showed PDCoV CH-01 strain was closely related to CHN-HB-2014 strain and HKU15-44 strain, while the other PDCoV Henan strains were more related to PDCoV CHJXNI2 and CH-SXD1-2015 strains, indicating that the ancestor of these sequenced strains may different. These results would support the understanding of the prevalence and evolution characteristics of PDCoV in China.

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“…It was proven experimentally that co-infections with PEDV and TGEV in cultured cells enhanced the damage to tight junctions and the remodeling of microfilaments compared with single infections with the same viruses (Belsham et al, 2016). Co-infections with PEDV and PDCoV in pigs have also be reported in several studies previously (Dong et al, 2015;Jang et al, 2017;Song et al, 2015). Before the outbreak of PEAV in farms in China in 2017, PEDV broke out in the same farms, indicating possible concurrent infections with PEDV and PEAV (Gong et al, 2017;Zhou et al, 2018).…”

Section: Discussionmentioning

confidence: 88%

Susceptibility of porcine IPI-2I intestinal epithelial cells to infection with swine enteric coronaviruses

Wang

Fang

Liu

et al. 2019

Veterinary Microbiology

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A B S T R A C TSwine enteric coronavirus (CoV) is an important group of pathogens causing diarrhea in piglets. At least four kinds of swine enteric CoVs have been identified, including transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and the emerging HKU2-like porcine enteric alphacoronavirus (PEAV). The small intestines, particularly the jejunum and ileum, are the most common targets of these four CoVs in vivo, and co-infections by these CoVs are frequently observed in clinically infected pigs. This study was conducted to investigate the susceptibility of the porcine ileum epithelial cell line, IPI-2I, to different swine enteric CoVs. We found that IPI-2I cells are highly susceptible to TGEV, PDCoV, and PEAV, as demonstrated by cytopathic effect and virus multiplication. However, only a small number of cells could be infected by PEDV, possibly due to the heterogeneity of IPI-2I cells. A homogeneous cell line, designated IPI-FX, obtained from IPI-2I cells by sub-cloning with limited serial dilutions, was found to be highly susceptible to PEDV. Furthermore, IPI-FX cells were also highly susceptible to TGEV, PDCoV, as well as PEAV. Thus, this sub-cloned IPI-FX cell line is an ideal cell model to study the mechanisms of infection, particularly co-infections of swine enteric CoVs.

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Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014-2016

Cited by 65 publications

References 23 publications

Detection and genetic characterization of porcine deltacoronavirus in Tibetan pigs surrounding the Qinghai-Tibet Plateau of China

Detection and genetic characterization of porcine deltacoronavirus in Tibetan pigs surrounding the Qinghai-Tibet Plateau of China

Prevalence, phylogenetic and evolutionary analysis of porcine deltacoronavirus in Henan province, China

Susceptibility of porcine IPI-2I intestinal epithelial cells to infection with swine enteric coronaviruses

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