Porcine Buccal Mucosa as an In Vitro Model: Relative Contribution of Epithelium and Connective Tissue as Permeability Barriers

Kulkarni, Upendra; Ravichandran, M.; Pather, Shaun; Li, Xiaoling; Jasti, Bhaskara

doi:10.1002/jps.21436

Cited by 54 publications

(42 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These lipids may undergo gel to liquid-crystalline transition, as was reported to occur in the skin's stratum corneum (58). In another reported study, no irreversible effects were found to occur in the lipid structure and function of canine buccal mucosa in the temperature range studied (59).…”

Section: Discussionmentioning

confidence: 70%

“…The thickness of the tissue was measured with the help of a digital screw gauge and recorded. This preparation procedure helped to minimize thickness variations between tissue specimens (58).…”

Section: Tissue Preparationmentioning

confidence: 99%

“…The tissues were allowed to equilibrate with buffer for 15 min before commencement of each permeability experiment. The permeability experiments were carried out using phosphate-buffered saline (PBS) with pH 7.4 in the receiver chamber and known concentrations of diffusant solutions at pH 6.8 in the donor chamber to mimic the in vivo physiological pH conditions (22,58). The receiver chamber was filled with 3.5 mL of PBS while 3.5 mL of the diffusant solution was charged into the donor chamber.…”

Section: Permeation Studiesmentioning

confidence: 99%

“…A reverse phase C18-column of 250 mm in length and 5 μm in internal diameter (Waters, Milford) was used to elute the diffusants. The HPLC conditions were developed and optimized for quantitative analysis of each individual diffusant as shown in Table II (3). The assay range for the four diffusants was established on the basis of relationship between the peak areas and the diffusant concentrations.…”

Section: Quantification Of Model Diffusantsmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Experimental Temperature on the Permeation of Model Diffusants Across Porcine Buccal Mucosa

Kulkarni

Ravichandran

et al. 2011

AAPS PharmSciTech

Self Cite

View full text Add to dashboard Cite

Abstract. The influence of experimental temperature on the permeability of model diffusants across porcine buccal mucosa was investigated in vitro. The permeability increased significantly as the experimental temperature was increased in increments of approximately 7°C. It was observed that the apparent permeability and temperature were related by an exponential relationship that conformed to the Arrhenius equation. Diffusants with higher lipophilicities-buspirone and bupivacaine-had lower activation energies for diffusion when compared to hydrophilic diffusants-antipyrine and caffeine. The activation energy for diffusion of the model diffusants decreased linearly with increasing distribution coefficients across porcine buccal mucosa. The results suggested that the buccal mucosa acts as a stronger barrier to the diffusion of hydrophilic diffusants than the lipophilic ones. The log-linear relationship between permeability and temperature indicates that temperature should be carefully controlled in diffusion experiments. These results also point to the possibility of developing heat-generating buccal delivery devices, especially for hydrophobic diffusants.

show abstract

Section: Discussionmentioning

confidence: 70%

Section: Tissue Preparationmentioning

confidence: 99%

Section: Permeation Studiesmentioning

confidence: 99%

Section: Quantification Of Model Diffusantsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Experimental Temperature on the Permeation of Model Diffusants Across Porcine Buccal Mucosa

Kulkarni

Ravichandran

et al. 2011

AAPS PharmSciTech

Self Cite

View full text Add to dashboard Cite

show abstract

“…Kulkarni et al (12) estimated a mucosal thickness of 500 µm as ideal for the simulation of natural mucosal barriers for diffusant permeation. However, Aran et al (6) set the thickness at 800 µm in their simulation studies and showed that the MucoJet had eight-fold higher capacity to deliver ovalbumin across the porcine buccal mucosa compared to the topical dropwise application.…”

mentioning

confidence: 99%