Populations of doubled haploids for genetic mapping in hexaploid winter triticale

Abstract

To create a framework for genetic dissection of hexaploid triticale, six populations of doubled haploid (DH) lines were developed from pairwise hybrids of high-yielding winter triticale cultivars. The six populations comprise between 97 and 231 genotyped DH lines each, totaling 957 DH lines. A consensus genetic map spans 4593.9 cM is composed of 1576 unique DArT markers. The maps reveal several structural rearrangements in triticale genomes. In preliminary tests of the populations and maps, markers specific to wheat segments of the engineered rye chromosome 1R (RM1B) were identified. Example QTL mapping of days to heading in cv. Krakowiak revealed loci on chromosomes 2BL and 2R responsible for extended vernalization requirement, and candidate genes were identified. The material is available to all parties interested in triticale genetics.Electronic supplementary materialThe online version of this article (10.1007/s11032-018-0804-3) contains supplementary material, which is available to authorized users.

Plant tissue culture environment as a switch-key of (epi)genetic changes

“…The plant in vitro cultures are of interest for the production of secondary metabolites of commercial interest (Loyola-Vargas and Ochoa-Alejo 2018). Plants obtained via PTC were used in genetic studies to generate mapping population for dissection of QTLs linked to agronomic traits (Tyrka et al 2018) and in studies of mobile elements (Shingote et al 2019). For a long time, in vitro culture methods were considered as the way of generating plants that are identical to the donor plants (Metakovsky et al 1987).…”

Participation of Wheat and Rye Genome in Drought Induced Senescence in Winter Triticale (X Triticosecale Wittm.)

“…The position of the selected 133 sequences with the best match indicated three physically remote locations in the wheat genome despite a small number of recombinations in the investigated triticale population. This is probably related to structural rearrangements within triticale chromosome 2A in relation to wheat as suggested in earlier works [15,20]. Considering large physical distance of the markers flanking QCSPhC.2A.3 in wheat, we limited our search for candidate genes to +/− 2cM region around the markers.…”

“…The genetic map covered 4907 cM with a mean distance between two bins of 3.0 cM. For QTL calculations, the orientation of genetic positions of the markers on chromosomes 2R, 4R and 5R was inverted based on a comparison with a published whole-rye genome draft sequence of rye [15,28].…”

“…), an intergeneric hybrid of wheat and rye with a genomic constitution of 2n = 6x = 42 (AABBRR), is mainly used as animal feed, but also in the production of renewable energy and very little in food sector. Triticale combines favorable agronomic characteristics of wheat (high yield potential, good grain quality) and rye (abiotic stress tolerance, disease resistance) [15]. The mechanisms associated with plant senescence under soil drought may be controlled by wheat and/or rye genome [16].…”

The QTL Mapping of the important breeding traits in winter triticale (×Triticosecale Wittm.)

“…Earlier maps of the rye genome (Korzun et al 1996) have been lately completed with high density DArT maps (Gawroński et al 2016). These circumstances helped to assemble triticale maps compiling various markers (Badea et al 2011;Alheit et al 2014;Tyrka et al 2011Tyrka et al , 2015Tyrka et al , 2018. Data on triticale QTL analyses are scarce so far (Niedziela et al 2012;Alheit et al 2014).…”