Population structure and genetic diversity in the invasive freshwater snail <i>Galba schirazensis</i> (Lymnaeidae)

Lounnas, Manon; Correa, Ana C.; Alda, Pilar; David, Patrice; Dubois, Marie‐Pierre; Calvopiña, Manuel; Caron, Yannick; Celi-Erazo, Maritza; Bui, Dung Thi; Jarne, Philippe; Loker, Eric S.; Noya, Óscar; Rodríguez‐Hidalgo, Richar; Toty, Céline; Uribe, Norma; Pointier, Jean‐Pierre; Hurtrez‐Boussès, Sylvie

doi:10.1139/cjz-2016-0319

Cited by 22 publications

(26 citation statements)

References 42 publications

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“…Considering all studies that were conducted earlier in the world ( Table 1 ) ( 1 , 2 , 4 ). Records of G. schirazensis were collected and entered an Excel database and then changed to a Comma-separated values (CSV) format for using in the model.…”

Section: Methodsmentioning

confidence: 99%

Molecular, Morphological, and Spatial Study of Galba schirazensis (Pulmonata, Lymnaeidae) from Southeastern Iran

Nasibi¹,

Moghaddam²,

Ziaali³

et al. 2021

IJPA

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Background: Snails of the genus Galba are the intermediate hosts of Fasciola species, the etiological agents of liver fluke disease, fascioliasis. A genetically different but morphologically very similar species in the genus, G. schirazensis, is sympatrically distributed with G. truncatula in some regions of the world. We aimed to investigate the occurrence of G. schirazensis in Kerman province, Iran and to characterize genetically G. schirazensis specimens from southeast Iran. Methods: Field-collected snails from four localities in Jiroft, Bam and Faryab, Kerman province, southeastern Iran were studied. Hydrological variables including temperature and pH were recorded for each habitat. Each specimen was identified using morphological as well as conchological characteristics. Genetic characterization was performed using PCR-sequencing followed by phylogenetic analyses on nuclear ITS2 as well as mitochondrial cox1 gene fragments. MaxEnt software was used to predict the most appropriate ecological niches for the targeted species. Results: G. schirazensis was found in 4 out of 28 locations. One ITS2 and two cox1 haplotypes were detected among G. schirazensis populations from the four localities. Habitat study showed that G. schirazensis thrives in habitats with alkaline pH. G.schirazensis from South America were clustered with specimens from Bam, Kerman, Iran; however, north Iranian isolates of G. schirazensis were strongly correlated with specimens from Jiroft and Faryab. MaxEnt model for the most appropriate ecological niches of the targeted species predicted environmental suitability for this species in western Africa as well as coastal areas in north and southwestern Africa. Conclusion: G. schirazensis is frequently present in southern areas of Kerman Province. At least two genetically different haplotypes are present in southeastern Iran.

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Section: Methodsmentioning

confidence: 99%

Molecular, Morphological, and Spatial Study of Galba schirazensis (Pulmonata, Lymnaeidae) from Southeastern Iran

Nasibi¹,

Moghaddam²,

Ziaali³

et al. 2021

IJPA

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“…We based our multiplex PCR method on species-specific primers amplifying microsatellite loci already described for the three targeted cryptic species: G. truncatula (Trouvé et al, 2000), G. cubensis (Lounnas et al, 2017b), and G. schirazensis (Lounnas et al, 2017a). We designed 11 primer mixes, each including one speciesspecific primer pair for each of the three species (Table S1).…”

Section: Methodsmentioning

confidence: 99%

“…Populations of Galba are small-shelled freshwater snails mostly originating in the Americas, subsequently invading Europe, Africa, and Asia (Bargues et al, 2011;Correa et al, 2011Correa et al, , 2010Lounnas et al, 2017a;Meunier et al, 2001). Their abilities to survive drought and to reproduce by self-fertilization allow them to disperse over long distances and establish new populations from single individuals (Bargues et al, 2011;Lounnas et al, 2017a, 2017b, Meunier et al, 2004. This high invasiveness has probably facilitated the worldwide expansion of fasciolosis.…”

Section: Introductionmentioning

confidence: 99%

“…Nor can they be distinguished with controlled crosses in the laboratory, as is possible in populations of the genus Physa (Dillon et al, 2011), because Galba populations typically demonstrate high selfing rates (Chapuis et al, 2007;Lounnas et al, 2017aLounnas et al, , 2017bMeunier et al, 2004). Thus, except for G. cousini adults with their distinct morphology, individuals of Galba have in recent years been identified using DNA sequencing technology (Correa et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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A new multiplex PCR assay to distinguish among three cryptic Galba species, intermediate hosts of Fasciola hepatica

Alda

Lounnas

Vázquez

et al. 2018

Veterinary Parasitology

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A molecular tool described here allows in one step for specific discrimination among three cryptic freshwater snail species (genus Galba) involved in fasciolosis transmission, a worldwide infectious disease of humans and livestock. The multiplex PCR approach taken targets for each species a distinctive, known microsatellite locus which is amplified using specific primers designed to generate an amplicon of a distinctive size that can be readily separated from the amplicons of the other two species on an agarose gel. In this way, the three Galba species (G. cubensis, G. schirazensis, and G. truncatula) can be differentiated from one another, including even if DNA from all three were present in the same reaction. The accuracy of this new molecular tool was tested and validated by comparing multiplex PCR results with species identification based on sequences at mitochondrial and nuclear markers. This new method is accurate, inexpensive, simple, rapid, and can be adapted to handle large sample sizes. It will be helpful for monitoring invasion of Galba species and for developing strategies to limit the snail species involved in the emergence or re-emergence of fasciolosis.

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“…The invasive potential of Lymnaeids species may be related to marked resistance to desiccation which increases their survival probability (Chapuis and Ferdy, 2012). When released in new environments, even a single individuals can develop a population as Lymnaeids can be self-fertilized (Escobar et al, 2011;Lounnas et al, 2018). Snails belonging to the Lymnaeidae family (Hurtrez-Boussès et al, 2001;Mas-Coma et al, 2005), such as G. truncatula and R. peregra, both introduced in the archipelago, are intermediary hosts for the Trematoda F. hepatica that contaminates part of São Miguel, Flores, and Santa Maria islands causing significant socioeconomic losses associated with damage to dairy livestock (Barbosa et al, 2019).…”

Section: Invertebratesmentioning

confidence: 99%

Non-indigenous and Invasive Freshwater Species on the Atlantic Islands of the Azores Archipelago

et al. 2021

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Freshwater systems on remote oceanic islands are particularly vulnerable to biological invasions. The case of freshwater ecosystems in the Azores Archipelago is especially relevant considering the islands’ youth and remoteness, and low natural connectivity. This study presents a review of the introduction and presence of non-indigenous freshwater species in the Azores, retrieved from various historical records, paleoenvironmental reconstructions, published records, and field data from two decades of the Water Framework Directive (WFD) monitoring programs. At least 132 non-indigenous freshwater species have successfully established in the Azores, belonging to several taxonomic groups: cyanobacteria (10), synurophytes (1), desmids (1), diatoms (20), plants (41), invertebrates (45), amphibia (2), and fishes (12). Intentional and accidental introductions have been occurring since the establishment of the first human settlers on the archipelago, impacting freshwater ecosystems. The first reported introductions in the Azores were intentional fish stocking in some lakes. Non-deliberate introductions have recently increased through transport-contaminants (51%) associated with the aquarium trade or agricultural products. In the Azores, the highest number of non-indigenous species occur on the largest and most populated island, São Miguel Island (116), followed by Flores (68). Plants constitute the most representative group of introduced species on all islands, but invertebrates, diatoms, and fishes are also well established on most islands. Among invertebrates, non-indigenous arthropods are the most well-established group on all islands except on the smallest Corvo Island. Many non-indigenous species will likely benefit from climate change and magnified by globalization that increases the probability of the movement of tropical and subtropical species to the Azores. Present trends in international trade, importations, and enhanced connectivity of the archipelago by increasing flights and shipping will probably promote the arrival of new species. Augmented connectivity among islands is likely to improve non-indigenous species dispersal within the archipelago as accidental transportation seems to be an essential pathway for non-indigenous freshwater species already present in the Azores.

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Population structure and genetic diversity in the invasive freshwater snail Galba schirazensis (Lymnaeidae)

Cited by 22 publications

References 42 publications

Molecular, Morphological, and Spatial Study of Galba schirazensis (Pulmonata, Lymnaeidae) from Southeastern Iran

Molecular, Morphological, and Spatial Study of Galba schirazensis (Pulmonata, Lymnaeidae) from Southeastern Iran

A new multiplex PCR assay to distinguish among three cryptic Galba species, intermediate hosts of Fasciola hepatica

Non-indigenous and Invasive Freshwater Species on the Atlantic Islands of the Azores Archipelago

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