Population growth and physiological characteristics of microalgae in a miniaturized bioreactor during space flight

Wang, Gaohong; Chen, Haofeng; Li, Genbao; Chen, Lanzhou; Li, Dunhai; Hu, Chunxiang; Chen, Kun; Liu, Yongding

doi:10.1016/j.actaastro.2005.11.001

Cited by 57 publications

(19 citation statements)

References 14 publications

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“…Results showed that the constant rotation of clinorotation treatment might act as a kind of stress and influenced the quantum yield of PSII photochemistry in Synechocystis. Similar results of decreased photosynthetic fluorescence efficiency in micro-algae and Brassica rapa plants in space microgravity condition were also found in Wang et al (2006) and Kochubey et al (2004). A decrease in photosys- tem efficiency may also be the result of mechanical injuries due to clinorotation.…”

Section: Resultssupporting

confidence: 72%

Physiological Responses of Synechocystis sp. PCC 6803 under Clinorotation

Wang

et al. 2012

Microgravity Sci. Technol.

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Photosystem efficiency and the characteristic on oxidative stress were examined to elucidate the metabolic responses of Synechocystis sp. PCC 6803 to short-term clinorotation. Results compiled when using clinostat to simulate microgravity for 60 h, showed that clinorotation clearly prohibited the photochemical quantum yield, but promoted the synthesis of chlorophyll and total protein. This may be a compensatory mechanism for the algal cell to maintain its normal metabolism. An increased malondialdehyde (MDA) content of algal cell upon clinorotation, together with an enhanced catalase (CAT) activity was observed during the whole period of clinorotation. One conclusion is that short-term clinorotation acts as a kind of stress, and that these physiological responses may be a special way for an algal cell to adapt itself to a different environment other than earth gravity.

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Section: Resultssupporting

confidence: 72%

Physiological Responses of Synechocystis sp. PCC 6803 under Clinorotation

Wang

et al. 2012

Microgravity Sci. Technol.

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“…As running lab experiments in space is a complex process and very expensive, much of the research is done on Earth with space simulations. Clinostats can simulate microgravity conditions and have promoted the development of space biology (Liu et al, 1993;Wang et al, 2006;Olsson-Francis & Cockell, 2010;Xiao et al, 2010). Although clinostats have been widely used to simulate microgravity conditions, most of the studies have concentrated on the effect of microgravity on tissues and cells of higher plants and mammals.…”

Section: Introductionmentioning

confidence: 99%

Cadmium uptake capacity of an indigenous cyanobacterial strain, Nostoc entophytum ISC32: new insight into metal uptake in microgravity-simulating conditions

et al. 2016

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Among nine cyanobacterial strains isolated from oil-contaminated regions in southern Iran, an isolate with maximum cadmium uptake capacity was selected and identified on the basis of analysis of morphological criteria and 16S rRNA gene sequence similarity as Nostoc entophytum (with 99 % similarity). The isolate was tentatively designated N. entophytum ISC32. The phylogenetic affiliation of the isolates was determined on the basis of their 16S rRNA gene sequence. The maximum amount of Cd(II) adsorbed by strain ISC32 was 302.91 mg g 21 from an initial exposure to a solution with a Cd(II) concentration of 150 mg l 21 . The cadmium uptake by metabolically active cells of cyanobacterial strain N. entophytum ISC32, retained in a clinostat for 6 days to simulate microgravity conditions, was examined and compared with that of ground control samples. N. entophytum ISC32 under the influence of microgravity was able to take up cadmium at amounts up to 29 % higher than those of controls. The activity of antioxidant enzymes including catalase and peroxidase was increased in strain ISC32 exposed to microgravity conditions in a clinostat for 6 days, as catalase activity of the cells was more than three times higher than that of controls. The activity of the peroxidase enzyme increased by 36 % compared with that of the controls. Membrane lipid peroxidation was also increased in the cells retained under microgravity conditions, up to 2.89-fold higher than in non-treated cells. Images obtained using scanning electron microscopy showed that cyanobacterial cells form continuous filaments which are drawn at certain levels, while the cells placed in a clinostat appeared as round-shaped, accumulated together and distorted to some extent.

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“…Microalgae need relatively small space and simple medium for growth, so they are very suitable for the space culture (Dixon et al, 1999). Microalgae bioreactor is a very efficient system to produce large amounts of protein in short time (Wang et al, 2006). The microalgae need CO 2 and light energy for photosynthesis, which can be coupled with oxygen production.…”

Section: Introductionmentioning

confidence: 99%