Population genetics of<i>Mycobacterium tuberculosis</i>complex in Scotland analysed by pulsed-field gel electrophoresis

Olson, Edwin S.; Forbes, Kenneth James; Watt, B.; Pennington, T. H.

doi:10.1017/s0950268800052006

Cited by 12 publications

(19 citation statements)

References 42 publications

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“…In contrast, Zhang et al [21], using Xbal in PFGE analysis of isolates from the USA, found greater strain diversity. This was interpreted as arising from the more diverse origin of the human population, possibly due to migration, compared to Scotland [22]. In the current study, Xbul was shown to distinguish between unrelated strains cultured from ethnically different populations.…”

Section: Discussionmentioning

confidence: 48%

“…Some isolates with the same pattern were isolated from patients in different Australian states, but no one strain predominated. Most isolates cultured from patients born in Australia and Northern Europe produced more DNA bands (19)(20)(21)(22) than isolates cultured from patients born in Asian countries such as the . However, a high degree of polymorphism was found amongst isolates of both groups.…”

Section: Resultsmentioning

confidence: 99%

“…In a previous study, Olson et al [22] reported that 75% of the isolates they examined could not be digested with Drul or gave smears of DNA on electrophoresis. The different results obtained in this study may be attributed to the use of different methods of DNA extraction.…”

Section: Discussionmentioning

confidence: 99%

“…Digestion with Xbu 1 identified less polymorphism than with Drul. This enzyme has been used previously to differentiate isolates of the M. tuberculosis complex from Scotland [22], but little polymorphism was reported. In contrast, Zhang et al [21], using Xbal in PFGE analysis of isolates from the USA, found greater strain diversity.…”

Section: Discussionmentioning

confidence: 99%

“…The technique has been used previously to study the population genetics of the M. tuberculosis complex, and for differentiation of isolates from the USA and the UK [21,22]. The Australian isolates of M. tuberculosis examined in this study were characterised previously by RFLP with pTBN12 and IS6110 as probes [17,23,and Edwards R,unpublished data], and in the current study these results were compared with those obtained by PFGE.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Differentiation of Australian Isolates of Mycobacterium Tuberculosis by Pulsed-Field Gel Electrophoresis

Feizabadi

Robertson²,

Edwards³

et al. 1997

Journal of Medical Microbiology

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As part of an epidemiological study of tuberculosis in Australia, 84 isolates of Mycobacteriuin tuberculosis from patients were analysed by pulsed-field gel electrophoresis (PFGE). The isolates were genetically heterogeneous, with 66 different DNA banding patterns obtained following digestion of genomic DNA with Dral and 53 patterns with Xbal. When the results were compared with those previously obtained in restriction fragment length polymorphism analysis (RFLP), in 87% of cases the results with Dral were consistent with those obtained with insertion sequence IS6110 as a probe in RFLP. However, PFGE was able to differentiate four of eight isolates which were identical with IS6110 typing. The high polymorphism amongst strains and the high average age of the patients (51 years) suggested that most organisms were cultured from patients who had reactivation of existing infections. Isolates with identical DNA patterns were found in different states of Australia, but no one strain predominated in any area. This suggests that tuberculosis has been introduced into

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Section: Discussionmentioning

confidence: 48%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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