Genetic Differentiation of Australian Isolates of Mycobacterium Tuberculosis by Pulsed-Field Gel Electrophoresis

Feizabadi, Mohammad M.; Robertson, Ian D.; Edwards, Rachel; Cousins, Debby; Hampson, David J.

doi:10.1099/00222615-46-6-501

Cited by 9 publications

(11 citation statements)

References 18 publications

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“…This hypothesis was supported by the PGRS probe, since isolates from old patients were also related when they were subjected to this probe. Previous studies showed the PGRS probe to be a powerful tool that can differentiate between strains that lack the IS6110 sequence and those that contain a few copies of this genetic element (10,11). The numbers of IS6110 and PGRS copies in the isolates cultured from old patients in Tehran were higher than those cultured from refugees and immigrants.…”

Section: Discussionmentioning

confidence: 99%

Multidrug-resistant Strains of Mycobacterium tuberculosis Isolated from Patients in Tehran Belong to a Genetically Distinct Cluster

Feizabadi

Shahriari

Safavi

et al. 2003

Scandinavian Journal of Infectious Diseases

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Restriction fragment length polymorphism (RFLP) analysis was used to study the molecular epidemiology of tuberculosis (TB) in certain areas of Tehran. 120 isolates of Mycobacterium tuberculosis, including drug-resistant strains (n = 23), were analysed using polymorphic GC-rich sequence (PGRS) and IS6110 probes. There was considerable diversity among the strains cultured from patients from certain areas. The results of RFLP showed that multidrug resistant (MDR) isolates of M. tuberculosis in Tehran belong to a group of strains with low copies of IS6110 and PGRS. The degree of clustering was higher for the drug-resistant strains than for the susceptible ones (65% vs 20%). Based on the demographic data and results of RFLP, it appears that recent transmissions of TB from old patients have occurred in Tehran. However, drug-resistant TB in the city is mainly caused by strains that look different from those cultured from such patients. The majority of MDR isolates (85%) in this study contained a low copy number of IS6110 and PGRS in RFLP, and were mostly recovered from immigrants and refugees.

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Section: Discussionmentioning

confidence: 99%

Multidrug-resistant Strains of Mycobacterium tuberculosis Isolated from Patients in Tehran Belong to a Genetically Distinct Cluster

Feizabadi

Shahriari

Safavi

et al. 2003

Scandinavian Journal of Infectious Diseases

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“…PFGE typing has successfully been used to differentiate between strains of M. tuberculosis (159), M. bovis (160), and M. bovis BCG (161). The reproducibility and discriminatory power of PFGE-based methods are high, although their use seems to be restricted to scientific or reference laboratories.…”

Section: Methods Based On Nonrepetitive Sequencesmentioning

confidence: 99%

“…PFGE is expensive and technically demanding, has a long turnover time (usually about 5 days), and, similarly to RFLP, requires large amounts of high-quality DNA. Moreover, discrimination between strains might not always be produced (159,162,163). The above-mentioned limitations discourage the use of PFGE for molecular epidemiological studies of MTBC infections.…”

Section: Methods Based On Nonrepetitive Sequencesmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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SUMMARYMolecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. With the advent of fingerprinting techniques, many traditional concepts regarding transmission, infectivity, or pathogenicity of mycobacterial bacilli have been revisited, and their conventional interpretations have been challenged. Since the mid-1990s, when the first typing methods were introduced, a plethora of other modalities have been proposed. So-called molecular epidemiology has become an essential subdiscipline of modern mycobacteriology. It serves as a resource for understanding the key issues in the epidemiology of tuberculosis and other mycobacterial diseases. Among these issues are disclosing sources of infection, quantifying recent transmission, identifying transmission links, discerning reinfection from relapse, tracking the geographic distribution and clonal expansion of specific strains, and exploring the genetic mechanisms underlying specific phenotypic traits, including virulence, organ tropism, transmissibility, or drug resistance. Since genotyping continues to unravel the biology of mycobacteria, it offers enormous promise in the fight against and prevention of the diseases caused by these pathogens. In this review, molecular typing methods forMycobacterium tuberculosisand nontuberculous mycobacteria elaborated over the last 2 decades are summarized. The relevance of these methods to the epidemiological investigation, diagnosis, evolution, and control of mycobacterial diseases is discussed.

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“…Finally, no standardized procedure for performing PFGE has yet been recommended. Despite these disadvantages, the PFGE typing was successfully used to differentiate between strains of M. tuberculosis [26], M. bovis [27], and M. bovis BCG [28]. However, the PFGE typing is rarely used in M. tuberculosis complex due to technical, time, and cost considerations, as mentioned above.…”

Section: Pulsed-field Gel Electrophoresis (Pfge)mentioning

confidence: 99%

“…However, the PFGE typing is rarely used in M. tuberculosis complex due to technical, time, and cost considerations, as mentioned above. Moreover, PFGE analysis does not always generate sufficient discrimination between the strains [26, 29, 30]. …”

Section: Pulsed-field Gel Electrophoresis (Pfge)mentioning

confidence: 99%

Current Methods in the Molecular Typing ofMycobacterium tuberculosisand Other Mycobacteria

Jagielski

Ingen

Rastogi

et al. 2014

BioMed Research International

121

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In the epidemiology of tuberculosis (TB) and nontuberculous mycobacterial (NTM) diseases, as in all infectious diseases, the key issue is to define the source of infection and to disclose its routes of transmission and dissemination in the environment. For this to be accomplished, the ability of discerning and tracking individual Mycobacterium strains is of critical importance. Molecular typing methods have greatly improved our understanding of the biology of mycobacteria and provide powerful tools to combat the diseases caused by these pathogens. The utility of various typing methods depends on the Mycobacterium species under investigation as well as on the research question. For tuberculosis, different methods have different roles in phylogenetic analyses and person-to-person transmission studies. In NTM diseases, most investigations involve the search for environmental sources or phylogenetic relationships. Here, too, the type of setting determines which methodology is most suitable. Within this review, we summarize currently available molecular methods for strain typing of M. tuberculosis and some NTM species, most commonly associated with human disease. For the various methods, technical practicalities as well as discriminatory power and accomplishments are reviewed.

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Genetic Differentiation of Australian Isolates of Mycobacterium Tuberculosis by Pulsed-Field Gel Electrophoresis

Cited by 9 publications

References 18 publications

Multidrug-resistant Strains of Mycobacterium tuberculosis Isolated from Patients in Tehran Belong to a Genetically Distinct Cluster

Multidrug-resistant Strains of Mycobacterium tuberculosis Isolated from Patients in Tehran Belong to a Genetically Distinct Cluster

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Current Methods in the Molecular Typing ofMycobacterium tuberculosisand Other Mycobacteria

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