Population genetics of 30 INDELs in populations of Poland and Taiwan

Abstract

The Investigator DIPplex® kit (Qiagen) contain components for the simultaneous amplification and analysis of 30 biallelic autosomal INDELs and amelogenin. The objective of this study was to estimate the diversity of the 30 markers in Polish (NP = 122) and Taiwanese (NT = 126) population samples and to evaluate their usefulness in forensic genetics. All amplicon lengths were shorter than 160 base pairs. The DIPplex genotype distributions showed no significant deviation from Hardy–Weinberg rule expectations (Bonferroni corrected) except for DLH39 in the Taiwanese population. Among the Poles and the Taiwanese the mean observed heterozygosity values are 0.4385 and 0.4079, and the combined matching probability values are 7.98 × 10−14 and 1.22 × 10−11, respectively. The investigated marker set has been confirmed as a potential extension to standard short tandem repeat-based kits or a separate informative system for individual identification and kinship analysis. Eight INDELs have been selected as possible ancestry informative single-nucleotide polymorphisms for further analyses.

Study of InDel genetic markers with forensic and ancestry informative interest in PALOP’s immigrant populations in Lisboa

“…They are characterized by the presence or absence of a specific sequence of nucleotides [9][10][11][12]. Significative differences in allele frequencies of InDel markers, between different groups or populations, can be used as ancestry and eventually, evolutionary indicators [13].…”

“…This kit allows the study of 30 InDel markers and the homologous gene of amelogenin as informative of the individual's sexual gender [12,16,17]. This multiplex has the advantage that amplified products can be separated and analyzed by capillary electrophoresis using an automatic sequencer, with similar technology to STR analysis.…”

“…The Investigator® DIPplex kit (Qiagen, Hilden, Germany) has already been employed in some population studies, specially in populations of European, American and Asian origin [8][9][10]12,[16][17][18][19][20][21][22][23][24]. Demonstrating high discrimination power and high power of exclusion, has shown to be proper for forensic cases, specially, in individual identification that require analysis of degraded samples.…”

Population Genetics and Forensic Efficiency of 30 InDel Markers in Four Chinese Ethnic Groups Residing in Sichuan

“…Insertion-deletion (InDel) polymorphisms has been a promising and powerful supplementary tool in forensic personal identification cases and kinship testing, since they bear the advantages of lower mutation rate, smaller amplicons and absence of stutter peaks compared with traditional gold standard short tandem repeats (STRs) [1][2][3][4][5].…”

Forensic performance of Investigator DIPplex indels genotyping kit in native, immigrant, and admixed populations in South Africa

“…It is composed of Amelogenin and 30 indel markers shorter than 160 bp with biallelic size range variation from 4 to 14 bp, located in the coding region of 19 autosomes. Since its launch, several studies have been conducted to evaluate its forensic performance and population polymorphisms in America, Europe, and Asia . However population data for indels is limited for African populations.…”