Population genetic structure of the tiger prawn (Penaeus monodon) in the coastal waters of South China, based on mitochondrial DNA control region sequences

Abstract

Summary Genetic variation and population structure of Penaeus monodon in the coastal waters of South China were detected using mitochondrial DNA control region sequences. Eighty individuals were collected at Sanya, Shenzhen, Zhanjiang and Beihai; 69 haplotypes with 157 polymorphic sites were detected. Nucleotide diversity (π) of the combined samples (6.16 ± 3.01%) was much higher than many other species in Chinese seas, such as Penaeus japonicus, Portunus trituberculatus, and Acanthopagrus schlegeli. Genetic differentiation was significant between Beihai and Sanya (pairwise FST = 0.09836, P < 0.05), and between Beihai and Shenzhen (pairwise FST = 0.12153, P < 0.05). Significant genetic differentiation among all populations was found by analysis of molecular variance (amova) (FST = 0.053, P = 0.037 < 0.05). The upgma dendrogram of the four populations showed Sanya and Shenzhen as the closest to each other, with Beihai having the greatest genetic distance from Sanya and Shenzhen. The tiger prawn of the coastal waters of South China should therefore be bred as two separated stocks, avoiding inbreeding or outbreeding selection of P. monodon in the captive breeding program. According to our results one source population is Beihai, and the others are from Sanya and Shenzhen.

Mitogenomic variation of Bangladesh Penaeus monodon (Decapoda, Panaeidae) and reassessment of its phylogeography in the Indo-West Pacific region

“…The high haplotype diversity in Bangladesh is similar as observed elsewhere in the Indo-West Pacific region where it ranges from 0.97 to 1.00 (You et al, 2008;Zhou et al, 2009;Waqairatu et al, 2012;Mkare et al, 2014). The nucleotide diversity (p) in Bangladesh is also comparable to the most samples with a single CR lineage, and it is close to the average of the samples characterized with the C lineage but nominally lower than within the samples characterized by the A lineage, south and east of the Sunda Shelf (Table 3).…”

“…The sequences were aligned using ClustalW Multiple alignment of Bioedit Sequence Alignment Editor (Hall, 1999) and were trimmed to 496 bp fragment, the sequences from the study of Walther et al (2011) and Mkare et al (2014) were aligned for 225 bps. The sequences of the CR were classified into the different lineages (A, B and C), as defined by Zhou et al (2009) and Walther et al (2011), by constructing a maximum likelihood tree with partial deletion treatment for missing data and a general time reversible (GTR) model with gamma and invariant sites parameters (G?I) in MEGA ver. 6.06 (Tamura et al, 2013) (tree not shown).…”

“…Penaeus monodon is known for high variation in molecular markers, which is geographically structured in the Indo-West Pacific region (Benzie et al, 2002;Kumar et al, 2007;You et al, 2008;Zhou et al, 2009;Mandal et al, 2012;Waqairatu et al, 2012). Variation in the mitochondrial DNA has been widely assessed for taxonomic identification and in phylogeographic and population genetic studies e.g.…”

“…The high mtDNA diversity in P. monodon was proposed by Walther et al (2011) to result from a paralogous duplication event but studies of the mitochondrial CR defined three distinct lineages across the species distribution (A, B and C); A was mostly found in the Pacific Ocean region, B from the juncture of the Indian-West Pacific Oceans and C from India and Africa (Zhou et al, 2009;Walther et al, 2011). Walther et al (2011) detected different haplotypes within a subset of individuals from Indonesia and concluded that these could have arisen due to duplication, either due to insertions of mitochondrial sequences into the nuclear genome (numts) or within the mitochondrial genome, and concluded that previous studies had thus highly overestimated the mtDNA variation.…”

GENETIC STATUS, SOURCE AND ESTABLISHMENT RISK OF THE GIANT TIGER SHRIMP (PENAEIDAE: Penaeus monodon), AN INVASIVE SPECIES IN COLOMBIAN CARIBBEAN WATERS

“…Even when we compare the data from the invasive population with other studies of native populations (e.g. Kumar et al, 2007;You et al, 2008;Zhou et al, 2009;Walther et al, 2011;Waqairatu et al, 2012), their genetic diversity remains low (Table 3). Genetic diversity loss associated with a founder effect has been observed in other invading organisms.…”