Population dynamics of bacteria including antifungal species in the rhizosphere of oilseed rape during its life cycle

Berg, Gabriele; Marten, P.; Bahl, Hubert

doi:10.1080/03235409809383231

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…Further studies have shown that overproduction of the inducible T. harzianum extracellular protease resulted in enhanced biocontrol efficacy against the fungal pathogen Rhizoctonia solani (Flores et al, 1997). The Gram-negative rod Stenotrophomonas maltophilia (formerly Xanthomonas maltophilia) is a typical soil inhabitant (Bollet et al, 1995 ;Berg et al, 1998) which has been the focus of scientific interest due to its potential for bioremediation (Boonchan et al, 1998) and for biological control Jakobi et al, 1996 ;Giesler & Yuen, 1998). S. maltophilia W81 is a sugar beet rhizosphere isolate capable of conferring protection against Py.…”

Section: Introductionmentioning

confidence: 99%

Overproduction of an inducible extracellular serine protease improves biological control of Pythium ultimum by Stenotrophomonas maltophilia strain W81

et al. 2000

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Stenotrophomonas maltophilia W81 can protect sugar beet against Pythiummediated damping-off disease through the production of an extracellular protease. Here, the proteolytic enzyme of W81 was purified by anion-exchange chromatography and characterized as a serine protease. The purified enzyme was fungicidal against Pythium ultimum in vitro. Its synthesis was inducible by casein in W81, and mutagenesis of this strain using the luciferase (luxAB) reporter transposon Tn5-764cd resulted in the isolation of two mutant derivatives (W81M3 and W81M4) capable of producing significantly increased levels of extracellular protease in the presence of casein. Strain W81M4 also exhibited increased chitinolytic activity. The luxAB fusions in strains W81M3 and W81M4 were highly expressed in the absence of casein but not in its presence, suggesting that the corresponding loci were involved in downregulating extracellular protease production. Extracellular protease production in the W81 wild-type strain and protease overproduction in mutants W81M3 and W81M4 were also induced in the presence of the autoclaved fungal mycelium. In soil microcosms naturally infested by Pythium spp., inoculation of sugar beet seeds with W81M3 or W81M4 resulted in improved biocontrol of Pythium-mediated damping-off disease compared with W81, and the level of protection achieved was equivalent to that conferred by chemical fungicides. The wild-type W81 and its mutant derivatives did not differ in rhizosphere colonization. Therefore, the improved biocontrol ability of W81M3 and W81M4 resulted from their capacity to overproduce extracellular serine protease.

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Section: Introductionmentioning

confidence: 99%

Overproduction of an inducible extracellular serine protease improves biological control of Pythium ultimum by Stenotrophomonas maltophilia strain W81

et al. 2000

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“…Pseudsomonas, Bacillus and Stenotrophomonas maltophilia, showed antifungal activity against Verticillium dahliae var. longisporum in vitro and were evaluated as potential biocontrol agents by 22 Pseudomonads represents the major group of nondifferentiating microorganisms that produce antibiotics such as phycocyanin, pyrolnitrin and pseudomonic acid, was investigated in in vitro and in vivo that showed anticandidal activity against Candida species 30 . Thomashow et al, 31 showed that the production of phenazine-1-carboxylic acid by P. fluorescens in the rhizosphere of wheat was correlated with take-all disease control.…”

Section: Resultsmentioning

confidence: 99%

Screening for Antifungal Activity of <italic>Pseudomonas Fluorescens</italic> Against phytopathogenic fungi

2008

IJMB

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Antifungal activity of different strains of Pseudomonas fluorescens were tested against some plant pathogens such as Alternaria solani, Curvularia lunata, Fusarium sp., Bipolaris sp. and Helminthosporium sp. in in vitro. Different concentrations (1000, 2000, 3000, 4000 and 5000 μg/mL) of Pseudomonas fluorescens were used and maximum inhibition of spore germination of fungus starts from 2000 μg/mL. The result indicated that all the strains of Pseudomonas fluorescens presented a most significant value against all the phytopathogenic fungi. Out of the five strains studied, the best result was shown by PUR-46, which showed almost complete inhibition against Fusarium sp., Curvularia lunata and Bipolaris sp. followed by R1 and PSB-1 against fungus Helminthosporium and Curvularia lunata respectively at concentration of 5000 μg/mL. The lowest inhibition at 5000 μg/mL was shown by PSB-1 and R-1 against Bipolaris.

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“…In vitro evaluation of Pseudomonas spp showed antifungal activity against Verticillium dahliae var. longisporum as potential biocontrol agents (Berg et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity of secondary metabolites of Pseudomonas fluorescens isolates as a biocontrol agent of chocolate spot disease (Botrytis fabae) of faba bean in Ethiopia

Alemu¹,

Alemu²

2013

Afr. J. Microbiol. Res.

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Pseudomonas fluorescens isolates possess a variety of promising properties of antifungal activity of secondary metabolites which make it as a biocontrol agent. In the present study, 12 isolates of P. fluorescens were isolated from rhizospheric soil of faba bean crop evaluated for their antagonistic activity against chocolate spot disease (Botrytis fabae) of faba bean. P. fluorescens 10 (Pf 10) (88.1%) showed high antagonistic activity against B. fabae. All isolate of P. fluorescens were successfully employed in controlling chocolate spot disease of faba bean due to their antifungal metabolites. The antifungal compounds were extracted from all P. fluorescens isolates with equal volume of ethyl acetate, hexane and methanol. The antifungal compounds extracted with ethyl acetate, hexane and methanol from P. fluorescens 3 (Pf 3), P. fluorescens 8 (Pf 8), and P. fluorescens 3 (Pf 3), isolates at 0.1% concentration completely inhibited the mycelial growth of the pathogen respectively. Bio-primed faba bean seeds with isolates of P. fluorescens 9 (Pf 9) and P. fluorescens 10 (Pf 10) evaluated against B. fabae in vivo (pot culture) indicated the inhibitory effects to the pathogen and also showed the inducing properties to enhance the immune system of crop. Therefore, it can be concluded that the use of P. fluorescens 9 (Pf 9) and P. fluorescens 10 (Pf 10) of isolates could inhibit the mycelial growth and reduced the disease incidence, severity and infection processes of B. fabae and simultaneously increase the plant growth performance and yield of faba bean. These isolates can be used as potential biocontrol agents against B. fabae and also used as biofertilizers for the production of faba bean.

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Population dynamics of bacteria including antifungal species in the rhizosphere of oilseed rape during its life cycle

Cited by 6 publications

References 22 publications

Overproduction of an inducible extracellular serine protease improves biological control of Pythium ultimum by Stenotrophomonas maltophilia strain W81

Overproduction of an inducible extracellular serine protease improves biological control of Pythium ultimum by Stenotrophomonas maltophilia strain W81

Screening for Antifungal Activity of <italic>Pseudomonas Fluorescens</italic> Against phytopathogenic fungi

Antifungal activity of secondary metabolites of Pseudomonas fluorescens isolates as a biocontrol agent of chocolate spot disease (Botrytis fabae) of faba bean in Ethiopia

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