“…In this sense, chitosan is an option for mixing with other antifungal agents, such as secondary metabolites like the ones found in the free extracts in Pseudomonas fluorescens cells. This bacteria displays control effects over phytopathogenic fungi in plantations of cabbage (Brassica oleracea), alfalfa (Medicago sativa), wheat (Triticum aestivum), broad bean (Vicia faba) and others (Alemu and Alemu, 2013;Mishra and Arora, 2012;Yanes et al, 2012;) by producing extracellular metabolites, which can be recovered from the culture medium and have an antifungal effect, without the need of having the bacteria present (Pal and McSpadden, 2006) and tackle the limitations of the use of biomass. The aim of this study was to evaluate the antifungal effect of the combination of chitosan and P. fluorescens extracts on the control in vitro of mycelia and A. alternata conidia, as well as the incidence and severity on tomato plants in greenhouse as an alternative possibility to reduce the use of agrochemicals.…”