Population diversity of Staphylococcus aureus isolated from bovine mastitis in Brazilian dairy herds

Costa, Geraldo Márcio da; Paiva, Luciano Vilela; Figueiredo, Henrique César Pereira; Figueira, A.R.; Pereira, Ulisses de Pádua; Silva, N.

doi:10.1016/j.rvsc.2011.09.014

Cited by 17 publications

(19 citation statements)

References 8 publications

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“…Even if there is a trade of animals between these two areas, the results of this study suggest that CC spread more frequently within their area of origin. The differences in CC frequencies observed in comparison to other investigations and the significant variations in frequencies when herd location was considered confirm that S. aureus genetic characteristics may be related to geographical location (Costa et al, 2012;Piccinini et al, 2010;Sakwinska et al, 2011;Zecconi et al, 2006a). This latter observation has important implication for vaccine development, because effective vaccines should be able to be provide protection even in presence of strains with different genetic characteristics (Middleton et al, 2009).…”

Section: Distribution Of CC and Mrsasupporting

confidence: 61%

Pattern characterization of genes involved in non-specific immune response in Staphylococcus aureus isolates from intramammary infections

Mazzilli

Piccinini

Scali

et al. 2015

Research in Veterinary Science

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Staphylococcus aureus isolated from mammary gland are characterized by different genetic patterns. Ninety four isolates from 33 dairy herds were analyzed by the means of a microarray to investigate S. aureus virulence patterns and the distribution of genes believed to be involved in immune evasion. None of the 94 isolates considered were MRSA. However, 50% of the isolates belonged to complexes related to MRSA and to human diseases, while only about 25% of them can be considered as exclusively of bovine origin. The distribution of clonal complexes and the different gene patterns observed confirmed the presence of an influence of geographical localization. The assessment of the influence of genes related to immune evasion on quarter milk cell count showed as four of them showed to be significantly associated to an increase quarter milk SCC. These genes could be potential target for developing new vaccines against S. aureus.

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Section: Distribution Of CC and Mrsasupporting

confidence: 61%

Pattern characterization of genes involved in non-specific immune response in Staphylococcus aureus isolates from intramammary infections

Mazzilli

Piccinini

Scali

et al. 2015

Research in Veterinary Science

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“…This may be for reasons similar to those for the high MPN of coliform bacteria described above, associated with the manipulation of endogenous starter culture, because 30 to 50% of humans are asymptomatic carriers of coagulase-positive Staphylococcus (Stamford et al, 2006). Another factor may have been the high incidence of mastitis in Brazilian dairy herds (Costa et al, 2012).…”

Section: Microbiological Quality Of Endogenous Starter Culturesmentioning

confidence: 87%

Lactic acid microbiota identification in water, raw milk, endogenous starter culture, and fresh Minas artisanal cheese from the Campo das Vertentes region of Brazil during the dry and rainy seasons

Castro

Oliveira

Sant’Anna

et al. 2016

Journal of Dairy Science

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Minas artisanal cheese, produced in the Campo das Vertentes region of Brazil, is made from raw milk and endogenous starter cultures. Although this cheese is of great historical and socioeconomic importance, little information is available about its microbiological and physical-chemical qualities, or about its beneficial microbiota. This work was aimed at evaluating the qualities of the cheese and the components used for its production, comparing samples collected during the dry and rainy seasons. We also conducted molecular identification and isolated 50 samples of lactic acid bacteria from cheese (n=21), water (n=3), raw milk (n=9), and endogenous starter culture (n=17). The microbiological quality of the cheese, water, raw milk, and endogenous starter culture was lower during the rainy period, given the higher counts of coagulase-positive Staphylococcus and total and thermotolerant coliforms. Enterococcus faecalis was the lactic acid bacteria isolated most frequently (42.86%) in cheese samples, followed by Lactococcus lactis (28.57%) and Lactobacillus plantarum (14.29%). Lactobacillus brevis (5.88%), Enterococcus pseudoavium (5.88%), Enterococcus durans (5.88%), and Aerococcus viridans (5.88%) were isolated from endogenous starter cultures and are described for the first time in the literature. The lactic acid bacteria identified in the analyzed cheeses may inhibit undesirable microbiota and contribute to the safety and flavor of the cheese, but this needs to be evaluated in future research.

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“…Previous studies, which were concerned with investi gating the clonal diversity of S. aureus using the polymorphism of the 3'-terminal region of coa gene (11,27,41), showed a wide diversity in the S. aureus population and in the presence of predominant coagulotypes. The limited number of coagulotypes found in our study suggests that there is a strong selective pressure on the coa gene, besides the limited geographical location.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance and Molecular Analysis of Staphylococcus aureus Isolated from Cow's Milk and Dairy Products in Northeast Brazil

Silveira-Filho¹,

Luz²,

Campos³

et al. 2014

Journal of Food Protection

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This work aimed to assess the clonal distribution among 94 strains of Staphylococcus aureus isolated from cow's milk, raw cheese, and a milking machine in 12 dairy farms in northeast Brazil, by analyzing different typing methods and detecting resistance and toxigenic profiles. For the first time, isolates of this region were assessed simultaneously by the polymorphism of the 3'-end coa gene and 16S-23S rDNA, pulsed-field gel electrophoresis, antibiotic resistance phenotyping, and toxigenic arsenal. Although pulsed-field gel electrophoresis patterns showed a wider variation (discriminatory index 0.83) than the PCR-based methods, the internal transcribed spacer-PCR proved to be a useful and inexpensive procedure for conducting epidemiological surveys of S. aureus on a regional scale. Each dairy farm had its own resistance profile, and in two herds, 63% of the strains were multiresistant, probably due to the indiscriminate use of antibiotics in bovine mastitis treatment. No methicillin-resistant S. aureus strains were detected in this study; however, 93.6% of S. aureus strains harbored variable profiles of staphylococcal enterotoxin genes seg, seh, sei, and sej. Transcriptional analysis revealed that 53.3% of staphylococcal enterotoxin genes actually transcribed, pointing out the food poisoning risk of these dairy products to consumers in the region. Based on the detection of the most prevalent clones in a herd or region, appropriate antibiotic therapy and specific immunization can be used for the treatment and control of staphylococcal mastitis.

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Population diversity of Staphylococcus aureus isolated from bovine mastitis in Brazilian dairy herds

Cited by 17 publications

References 8 publications

Pattern characterization of genes involved in non-specific immune response in Staphylococcus aureus isolates from intramammary infections

Pattern characterization of genes involved in non-specific immune response in Staphylococcus aureus isolates from intramammary infections

Lactic acid microbiota identification in water, raw milk, endogenous starter culture, and fresh Minas artisanal cheese from the Campo das Vertentes region of Brazil during the dry and rainy seasons

Antibiotic Resistance and Molecular Analysis of Staphylococcus aureus Isolated from Cow's Milk and Dairy Products in Northeast Brazil

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