Population diversity and multiplicity of infection in Theileria annulata

Weir, William; Karagenç, Tülin; Gharbi, Mohamed; Simuunza, Martin; Aypak, Süleyman; Aysul, Nuran; Darghouth, Mohamed Aziz; Shiels, Brian; Tait, Andrew

doi:10.1016/j.ijpara.2010.08.004

Cited by 62 publications

(81 citation statements)

References 43 publications

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“…In almost every animal positive for T. annulata a high MOI was observed. Similar MOI levels were observed in Turkey and lower values were recorded in Tunisia (Weir et al, 2007(Weir et al, , 2011. MOI is an epidemiological measure of the number of parasite genotypes per infected individual, frequently used in malaria research, which can improve knowledge of parasite populations in aspects such as the dynamics of infection and as an indicator of immune status, pathogenesis, or the effect of transmission intensity and drug efficacy (Ross et al, 2012).…”

Section: Discussionmentioning

confidence: 68%

“…The number of co-infecting genotypes gradually increases over time, presumably as the animal is exposed to new genotypes. In contrasts to the previous study, which included a significant number of calves (Weir et al, 2011), the focus of sampling in the present study was on adult carrier animals and therefore the relationship between host age and MOI could not be explored. No association of MOI was detected with different cattle breeds, although the number of animals representing each breed was relatively low and therefore there was little statistical power to investigate this issue.…”

Section: Discussionmentioning

confidence: 96%

“…Host age was previously demonstrated to be a significant determinant of MOI in Turkey and Tunisia because of the increased tick exposure over time and multiple re-infections of animals during the tick season (Weir et al, 2011). The number of co-infecting genotypes gradually increases over time, presumably as the animal is exposed to new genotypes.…”

Section: Discussionmentioning

confidence: 99%

“…For the construction of multilocus genotypes (MLG) for each sample, we identified the predominant peak (predominant allele) at each locus in each sample and assigned it to a bin. The genotyping of a cloned parasite isolate (Ankara D7) in this study allowed the MLG dataset to be harmonized with and compared to a previous Tunisian and Turkish dataset (Weir et al, 2011).…”

Section: Genotyping and Allele Callingmentioning

confidence: 99%

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Population diversity of Theileria annulata in Portugal

Gomes

Salgueiro

Inácio

et al. 2016

Infection, Genetics and Evolution

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The tick-borne protozoan parasite Theileria annulata causes tropical theileriosis, a severe disease of cattle that occurs across the Mediterranean littoral, the Middle East and Southern Asia. In the Mediterranean region, the disease has long been perceived as being a constraint to livestock production in North Africa and Turkey but was believed to have minimal impact in Southern European countries. It has recently been demonstrated that in Southern Portugal the prevalence of T. annulata is approximately 30%. While the population genetics of the parasite and the multiplicity of infection in the bovine host have been studied in a number of countries, no information is currently available on the composition of the parasite population in Southern Europe or its relationship to populations in bordering regions. A parasite genotyping system, based on micro-and mini-satellite amplification, was used to perform genetic analysis of T. annulata populations from T. annulata infected cattle in twelve farms in Southern Portugal. A diversity of genotypes and a high multiplicity of infection were found, suggesting that the parasite possesses a panmictic population in this region. In comparison with genotypes found in Tunisia and Turkey, parasites from Portugal form a genetically distinct group and show lower genetic diversity.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Genotyping and Allele Callingmentioning

confidence: 99%

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Population diversity of Theileria annulata in Portugal

Gomes

Salgueiro

Inácio

et al. 2016

Infection, Genetics and Evolution

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“…Parazitin epidemiyolojisi üzerinde selektif etki oluşturan ilaç uygulamaları, koruyucu amaçla uygulanan aşılamalar, doğal koşullarda oluşan tekrarlı enfeksiyonlar, aşırı ve düzensiz akarisit kullanımı, kene habitatlarındaki değişimler ve kontrolsüz hayvan hareketleri gibi faktörler parazitin popülasyon yapısında genetik düzeyde farklılaşmaların oluşmasına neden olmaktadır (10)(11)(12)(13). Bu durum hastalığın epidemiyolojisi üzerine etki yaparak gelecekte parazitin farklı popülasyonlarından kaynaklanan yeni risklerin ortaya çıkmasına yol açabilmektedir.…”

Section: Introductionunclassified

Selection of Genetic Markers to Determine Diversity in Theileria annulata Populations after Recombination

Bilgiç¹,

Ünlü²,

Aksulu³

et al. 2017

TurkiyeParazitolDerg

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ÖZAmaç: Theileria annulata popülasyonlarında rekombinasyon sonrası oluşacak genetik çeşitliliğin ve rekombinasyonun yoğun olduğu kromozomal bölgelerin tespit edilmesi amacıyla kullanılacak polimorfik mini ve mikrosatellite markerlerin belirlenmesidir. Yöntemler: Markerlerin belirlenmesi amacıyla Unipro UGENE programı kullanılmıştır. T. annulata'nın rastgele karıştırılmış genomik dizilimlere göre gerçek DNA diziliminde 10 kat fazla tandem tekrar (TR) belirleyen skor, eşik değer olarak belirlenmiştir. Tekrarlı motif benzerliği %96-100 arasında bulunan, minimum tekrar sayısı en az üç ve minimum tandem boyutu minisatellite bölgeler için en az üç nükleotid ve mikrosatellite bölgeler için en az altı nükleotid olan TR'ler suffix array algoritması kullanılarak tüm genom boyunca taranmıştır. Bulgular: Tarama sonrası 359 minisatellite ve 8973 mikrosatellite bölge belirlenmiştir. TR'ler tüm genom boyunca tek tek taranarak; T. annulata'nın kromozomları üzerindeki yerleşim yerlerine, motiflerin benzerliğine (>%96), tekrar sayıları ile tek bölgeyi temsil etmesine, bölgelerin uzunluklarına (<1500 bp) ve primer tasarlanmaya uygun korunmuş bölgeler içermesine bakılarak mini ve mikrosatellite bölgeler belirlenmiştir. Belirlenen bölgeleri çoğaltmada kullanılacak primerler, korunmuş bölgelerden tasarlanmış ve her bir primer, T. annulata'nın farklı izolatları kullanılarak değerlendirilmiştir. Sonuç: Bu çalışmada, T.annulata popülasyonlarındaki çeşitliliğinin belirlenmesi amacıyla daha önce belirlenen markerlere ilave olarak kullanılabile-cek, farklı kromozomlar üzerinde bulunan toplam 13 mini ve mikrosatellite marker seçilmiştir. Anahtar Kelimeler: Theileria annulata, rekombinasyon, satellit marker Geliş Tarihi: 17.06.2016Kabul Tarihi: 21.12.2016 ABSTRACT Objective: Selecting polymorphic mini-and microsatellite markers to determine genetic diversity and chromosomal regions exhibiting elevated rates of recombination in Theileria annulata populations after recombination. Methods: The Unipro UGENE software was used to select markers. A score at which 10 times more tandem repeats (TRs) were identified in the real DNA sequence than those in the scrambled sequences of T. annulata was used as the cutoff. TRs containing minimum three nucleotides in length for microsatellite and six nucleotides for minisatellite regions and having a repeat motif identity between 96%-100% with the unit size repeated minimum three times were screened through the whole genome using the suffix array algorithm.Results: A total of 359 minisatellites and 8973 microsatellites were identified. TRs were screened one by one through the whole genome; mini-and microsatellites representing a single region and having suitable regions for primer design were selected based on their localization on T. annulata chromosomes, their repeat motif identity (>96%), and their repeat length (<1500 bp). The primers used to amplify selected candidates were designed, and each primer was used to check 27 different isolates of T. annulata. Conclusion: In the present study, a total of 13 po...

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Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia

Sallemi

Rjeibi

Rouatbi

et al. 2017

Veterinary Medicine & Sci

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The present study aimed to estimate the molecular prevalence of Theileria annulata and Trypanosoma evansi infection in cattle in Northern Tunisia. A total number of 96 cattle from five farms were evaluated. T. annulata and T. evansi prevalences were 61% [56/66] and 10% [7/13], respectively, at a confidence interval (CI) of 95%, while co‐infection was present in 6% [4/8] of the tested animals at a CI of 95%. There was a significant correlation between age and the prevalence of T. annulata infection, whereas, there was no significant association shown with the age of cattle and T. evansi infection. Sequence and phylogenetic analyses showed that the T. annulata Tams1 gene and T. evansi ITS1 rDNA gene were highly conserved with 97.1–100% and 98.3–100% sequence identity, respectively.

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Population diversity and multiplicity of infection in Theileria annulata

Cited by 62 publications

References 43 publications

Population diversity of Theileria annulata in Portugal

Population diversity of Theileria annulata in Portugal

Selection of Genetic Markers to Determine Diversity in Theileria annulata Populations after Recombination

Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia

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