Seasonal variation in diet of golden jackal in Serbia was assessed by stomach content analysis. During the period 2004During the period -2013 samples were collected at two localitiesSurčin (central Serbia, 15 km west of Belgrade, along river Sava) and Veliko Gradište (northeastern Serbia, 85 km east of Belgrade, along Danube River). Jackals consumed a broad range (15) of different food categories. Seasonal differences in diet are related with the availability of certain food categories. Small mammals are the most important food category when they are most abundant-in summer and autumn regarding both frequency of occurrence and frequency of biomass consumed (32.1 %O and 36.5 %B, and 29.0 %O and 37.0 %B, respectively). Moreover, high percentage of plant material is recorded during summer (23.8 %O, 20.8 %B), consisting mainly of fruit that ripen at this time of the year. Due to presence of domestic ungulate leftovers on illegal dumps year-round, this food category is representing its staple diet. High overlap between summer and autumn as well as between winter and spring is recorded (C=0.97 and C=0.94, respectively). These findings confirm golden jackal's opportunistic nature, foraging behaviour and high dependence on human-provided food resources.