Population-based study of the incidence of Shigella diarrhea and causative serotypes in Santiago, Chile

Prado, Valeria; Lagos, Rosanna; Nataro, James P.; Martin, Oriana San; Arellano, Carolina; Wang, Jin Yuan; Borczyk, A; Levine, Myron M.

doi:10.1097/00006454-199906000-00005

Cited by 21 publications

(15 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…"E.coli strain was the first most common group entero pathogen isolates (7.7) %. The relative prevalence of these categories diarrhea E.coli was similar to that observed among malnourished children [17]) and children with acute diarrhea in north Jorden [18]," and their presence in children with diarrhea in other developing countries has been documented [19] [20]. However, the findings of this study confirm the importance of "salmonella spp.…”

Section: Study Populationsupporting

confidence: 85%

Factors association with some bacteria cause diarrhea disease among children under 5 years old

Al-Mizury¹

2018

MJS

View full text Add to dashboard Cite

Diarrhea has been a common cause of morbidity and mortality in children under 5 years old. This study was intended to assessment level of personal hygiene, type of water taken by children, crowding index and some factors associated with some bacterial infection in children under 5 years." This study enrolled 143 children under 5 years with clinical evidence of diarrheal disease through the period extending from 15/4/2016 to 30/8/2016, who were admitted to Baghdad teaching hospital, stool samples were collected from children who had diarrhea were inoculated on selective culture media using standard method. "The isolate were identified depending on morphological feature of colonies and from all media biochemically using API 20E system." A total bacterial infection was observed (13.9%). Specific prevalence of species bacteria is as follows,"E.coli (7.7%), and Shigella spp. (2%), and Salmonella spp.(3.5%) and V. cholera (0.7%)." Finding from our study indicate that patient in the age group>5 years of age were more likely to have diarrhea than those who were younger, and Children that consumed tap water were more infected with bacteria (9.7%). In this study, crowding index were associated with diarrheal disease, "children from households with 1 or 2 people per room were (1.4%) less likely to have diarrhea compared to children from household with more than 3 people per room (30%). Our results indicate that availability of house hold sanitation facilities, access to filter and clean water, good personal hygiene and butter nutrition were all associated with lower incidence of diarrhea." Keywords

show abstract

Section: Study Populationsupporting

confidence: 85%

Factors association with some bacteria cause diarrhea disease among children under 5 years old

Al-Mizury¹

2018

MJS

View full text Add to dashboard Cite

show abstract

“…A total of 55 Shigella genomes were selected from our culture collection for sequencing in an effort to capture a broad range of genomic, geographic, and temporal diversity ( Table 1); 30 of these genomes were sequenced as part of the NIAID Genome Sequencing Center for Infectious Diseases (GSCID) project (http://gscid.igs.umaryland.edu /wp.php?wp ϭ emerging _diarrheal_pathogens). Additional sequenced isolates included a collection of Shigella isolates (n ϭ 12) from Nyanza Province, western Kenya, collected by the Kenya Medical Research Institute (KEMRI)/Centers for Disease Control and Prevention (CDC) Research and Public Health Collaboration that were associated with lethal clinical outcomes (19), a set of isolates from Chile (n ϭ 2) used in previous studies (20,21), and a collection of Canadian isolates (n ϭ 11) obtained from the Public Health Agency of Canada through routine surveillance from 2008 to 2011; all Shigella isolates were identified based on serological analyses. A list of 281 additional genomes downloaded from GenBank, including completed genomes as well as draft assemblies, as well as the reads for an additional 96 S. flexneri genomes for comparative analyses are listed in Table S1 in the supplemental material.…”

Section: Methodsmentioning

confidence: 99%

Defining the Phylogenomics of Shigella Species: a Pathway to Diagnostics

et al. 2015

Self Cite

View full text Add to dashboard Cite

g Shigellae cause significant diarrheal disease and mortality in humans, as there are approximately 163 million episodes of shigellosis and 1.1 million deaths annually. While significant strides have been made in the understanding of the pathogenesis, few studies on the genomic content of the Shigella species have been completed. The goal of this study was to characterize the genomic diversity of Shigella species through sequencing of 55 isolates representing members of each of the four Shigella species: S. flexneri, S. sonnei, S. boydii, and S. dysenteriae. Phylogeny inferred from 336 available Shigella and Escherichia coli genomes defined exclusive clades of Shigella; conserved genomic markers that can identify each clade were then identified. PCR assays were developed for each clade-specific marker, which was combined with an amplicon for the conserved Shigella invasion antigen, IpaH3, into a multiplex PCR assay. This assay demonstrated high specificity, correctly identifying 218 of 221 presumptive Shigella isolates, and sensitivity, by not identifying any of 151 diverse E. coli isolates incorrectly as Shigella. This new phylogenomics-based PCR assay represents a valuable tool for rapid typing of uncharacterized Shigella isolates and provides a framework that can be utilized for the identification of novel genomic markers from genomic data.

show abstract

“…S. dysenteriae causes one of the most severe forms of epidemic severe dysenteries. 26 It was most sensitive to ceftriaxone, ceftizoxime, cephalotine, and co-trimoxazole; and most resistant to kanamycin, amikacin and nalidixic acid.…”

Section: 25mentioning

confidence: 99%

Frequency and antimicrobial susceptibility of Shigella species isolated in Children Medical Center Hospital, Tehran, Iran, 2001-2006

Pourakbari¹,

Mamishi²,

Mashoori³

et al. 2010

Braz J Infect Dis

View full text Add to dashboard Cite

We declare no confl ict of interest. ABSTRACTAppropriate antimicrobial treatment of shigellosis depends on identifying its changing resistance pattern over time. We evaluated 15,255 stool culture submitted from July 2001 to June 2006 to the Laboratory of Children Medical Center Hospital. Specimen culture, bacterial identifi cation, and disk diffusion susceptibility testing were performed according to National Committee for Clinical Laboratory Standards guidelines. From 15,255 stool samples, 682 (4.5%) were positive for Shigella species. The most common species of Shigella were S. fl exneri (48%) and S. sonnei (45%); other results were S. dysenteriae (5%) and S. boydii (2%). The rate of Sensitivity to ceftriaxone (95%), ceftizoxime (94%), and nalidixic acid (84%) were among our isolates. Resistance to co-trimoxazole and ampicillin was 87% and 86%, respectively. S. fl exneri was more multiresistant than other species (47.9%). Our isolates are overall most sensitive to ceftriaxone, ceftazidime, and nalidixic acid (> 84%). They were most resistant to co-trimoxazole and ampicillin (> 86%). Because resistance varies according to specifi c location, continuous local monitoring of resistance patterns is necessary for the appropriate selection of empirical antimicrobial therapy.

show abstract

Population-based study of the incidence of Shigella diarrhea and causative serotypes in Santiago, Chile

Cited by 21 publications

References 29 publications

Factors association with some bacteria cause diarrhea disease among children under 5 years old

Factors association with some bacteria cause diarrhea disease among children under 5 years old

Defining the Phylogenomics of Shigella Species: a Pathway to Diagnostics

Frequency and antimicrobial susceptibility of Shigella species isolated in Children Medical Center Hospital, Tehran, Iran, 2001-2006

Contact Info

Product

Resources

About