Poor maternal nutrition inhibits muscle development in ovine offspring

Reed, Sarah; Raja, Joseline; Hoffman, M. L.; Zinn, S. A.; Govoni, Kristen E

doi:10.1186/2049-1891-5-43

Cited by 66 publications

(83 citation statements)

References 62 publications

(69 reference statements)

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“…Similarly, satellite cells isolated from the offspring of rats undernourished during gestation demonstrated formation of fewer muscle fibers (de Melo et al, 2011). Furthermore, RES offspring at 3 months of age have reduced muscle fiber cross sectional area compared with CON lambs (Reed et al, 2014), supporting inhibited muscle growth as a result of restricted maternal nutrition during gestation.…”

Section: Discussionmentioning

confidence: 91%

“…Pregnant ewes were transitioned onto a control (100% NRC requirements for TDN) or restricted-fed (60% NRC requirements) diet at day 31 ± 1.3 of gestation and remained on their respective diets until parturition as described in Reed et al (2014). At birth, lambs from control-fed ewes (CON, n = 12) and restricted-fed ewes (RES, n = 12) were allowed to nurse to receive colostrum for up to 24 h. One-half of the lambs from each diet group were necropsied within 24 h of birth.…”

Section: Methodsmentioning

confidence: 99%

“…Immediately after thawing, a Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA, USA) was used to extract RNA from satellite cells isolated from lambs at birth. Expression of Pax7, MyoD and myogenin was measured by real-time RT-PCR using iTaq SybrGreen Master Mix (BioRad, Hercules, CA, USA) and the ABI 7900HT Fast Real-time PCR machine (Applied Biosystems, Foster City, CA, USA) as previously described (Reed et al, 2014). Cell culture data were analyzed as a completely randomized design with treatments arranged as a 2 × 2 factorial with age of lamb and maternal diet as main effects.…”

Section: Methodsmentioning

confidence: 99%

“…Poor maternal nutrition during gestation results in reduced lean-to-fat ratio (Zhu et al, 2006), reduced muscle cross-sectional area (Reed et al, 2014) and increased fat deposition (Bee, 2004) in offspring. Insults to muscle progenitor cells, like poor maternal nutrition during prenatal development, likely contribute to these immediate and long-term negative outcomes.…”

Section: Introductionmentioning

confidence: 99%

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Restricted maternal nutrition alters myogenic regulatory factor expression in satellite cells of ovine offspring

Raja

Hoffman

Govoni

et al. 2016

Animal

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Poor maternal nutrition inhibits muscle development and postnatal muscle growth. Satellite cells are myogenic precursor cells that contribute to postnatal muscle growth, and their activity can be evaluated by the expression of several transcription factors. Pairedbox (Pax)7 is expressed in quiescent and active satellite cells. MyoD is expressed in activated and proliferating satellite cells and myogenin is expressed in terminally differentiating cells. Disruption in the expression pattern or timing of expression of myogenic regulatory factors negatively affects muscle development and growth. We hypothesized that poor maternal nutrition during gestation would alter the in vitro temporal expression of MyoD and myogenin in satellite cells from offspring at birth and 3 months of age. Ewes were fed 100% or 60% of NRC requirements from day 31 ± 1.3 of gestation. Lambs from control-fed (CON) or restricted-fed (RES) ewes were euthanized within 24 h of birth (birth; n = 5) or were fed a control diet until 3 months of age ( n = 5). Satellite cells isolated from the semitendinosus muscle were used for gene expression analysis or cultured for 24, 48 or 72 h and immunostained for Pax7, MyoD or myogenin. Fusion index was calculated from a subset of cells allowed to differentiate. Compared with CON, temporal expression of MyoD and myogenin was altered in cultured satellite cells isolated from RES lambs at birth. The percent of cells expressing MyoD was greater in RES than CON ( P = 0.03) after 24 h in culture. After 48 h of culture, there was a greater percent of cells expressing myogenin in RES compared with CON ( P < 0.001). After 72 h of culture the percent of satellite cells expressing myogenin in RES was less than CON ( P < 0.01). There were no differences in the gene expression of Pax7, Myf5 or MyoD in isolated satellite cells at birth ( P > 0.05). In satellite cells from RES lambs at 3 months of age, the percent of cells expressing MyoD and myogenin were greater than CON after 72 h in culture ( P < 0.05). Fusion index was reduced in RES lambs at 3 months of age compared with CON ( P < 0.001). Restricted nutrition during gestation alters the temporal expression of myogenic regulatory factors in satellite cells of the offspring, which may reduce the pool of myoblasts, decrease myoblast fusion and contribute to the poor postnatal muscle growth previously observed in these animals.

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Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Restricted maternal nutrition alters myogenic regulatory factor expression in satellite cells of ovine offspring

Raja

Hoffman

Govoni

et al. 2016

Animal

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“…Poor maternal nutrition can result from excess or reduced nutrient intake including energy, protein, and micronutrients in the diet. Poor maternal nutrition during gestation is known to reduce fetal growth [2–4], impair muscle development [5,6], reduce bone density [1,7], increase fat accretion [5,8,9], alter metabolism [2,3,10], and impair stem cell function [11,12] in the offspring.…”

Section: Introductionmentioning

confidence: 99%

Effects of Poor Maternal Nutrition during Gestation on Bone Development and Mesenchymal Stem Cell Activity in Offspring

et al. 2016

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Poor maternal nutrition impairs overall growth and development of offspring. These changes can significantly impact the general health and production efficiency of offspring. Specifically, poor maternal nutrition is known to reduce growth of bone and muscle, and increase adipose tissue. Mesenchymal stem cells (MSC) are multipotent stem cells which contribute to development of these tissues and are responsive to changes in the maternal environment. The main objective was to evaluate the effects of poor maternal nutirtion during gestation on bone and MSC function in offspring. Thirty-six ewes were fed 100%, 60%, or 140% of energy requirements [NRC, 1985] beginning at day 31 ± 1.3 of gestation. Lambs from ewes fed 100% (CON), 60% (RES) and 140% (OVER) were euthanized within 24 hours of birth (1 day; n = 18) or at 3 months of age (n = 15) and bone and MSC samples were collected. Dual X-ray absorptiometry was performed on bones obtained from day 1 and 3 months. Proliferation, differentiation, and metabolic activity were determined in the MSC isolated from lambs at day 1. Data were analyzed using mixed procedure in SAS. Maternal diet negatively affected offspring MSC by reducing proliferation 50% and reducing mitochondrial metabolic activity. Maternal diet did not alter MSC glycolytic activity or differentiation in culture. Maternal diet tended to decrease expression of P2Y purinoreceptor 1, but did not alter expression of other genes involved in MSC proliferation and differentiation. Maternal diet did not alter bone parameters in offspring. In conclusion, poor maternal diet may alter offspring growth through reduced MSC proliferation and metabolism. Further studies evaluating the potential molecular changes associated with altered proliferation and metabolism in MSC due to poor maternal nutrition are warranted.

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Effects of different maternal feeding strategies from day 1 to day 85 of gestation on glucose tolerance and muscle development in both low and normal birth weight piglets

Peng

Yang

et al. 2020

J Sci Food Agric

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BACKGROUND: Maternal nutrition during gestation plays a vital role in fetal development. The effects of different maternal feeding strategies from day 1 to day 85 of gestation on glucose tolerance and muscle development in low and normal birth weight offspring were investigated by using 80 gilts randomly allotted to T1 and T2 groups and treated respectively with a gradual-increase (T1) and a convex transition (T2) feeding strategy, with no difference in total feed intake. RESULTS: T2 group was seen to have a higher percentage of piglets with birth weight less than 500 g, while T1 group was shown to have a higher percentage of piglets with birth weight over 700 g. Meanwhile, for both low and normal birth weight piglets, T1 group was higher than T2 group in terms of muscle free amino acid concentration, mRNA expression levels of muscle growthrelated factors, relative muscle fiber number and cross-sectional area. We must emphasize that the T2 group was shown to improve glucose tolerance, slow-twitch muscle fiber protein levels, and muscle mitochondrial function only in low birth weight piglets. CONCLUSION: The convex transition feeding strategy can decrease the percentage of piglets with birth weight over 700 g, while improving glucose tolerance, slow-twitch muscle fiber protein levels, and muscle mitochondrial function in low birth weight piglets. Our findings provide new evidence for the potential importance of nutritional strategies during gestation, especially for improving the glucose tolerance and muscle development of low birth weight neonatal.

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Poor maternal nutrition inhibits muscle development in ovine offspring

Cited by 66 publications

References 62 publications

Restricted maternal nutrition alters myogenic regulatory factor expression in satellite cells of ovine offspring

Restricted maternal nutrition alters myogenic regulatory factor expression in satellite cells of ovine offspring

Effects of Poor Maternal Nutrition during Gestation on Bone Development and Mesenchymal Stem Cell Activity in Offspring

Effects of different maternal feeding strategies from day 1 to day 85 of gestation on glucose tolerance and muscle development in both low and normal birth weight piglets

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