Poor expression of miR‐195‐5p can assist the diagnosis of cerebral vasospasm after subarachnoid hemorrhage and predict adverse outcomes

Abstract

Objective: Patients with spontaneous subarachnoid hemorrhage (SAH) may develop refractory arterial cerebral vasospasm (CVS), which is the leading cause of death in SAH patients. This study explored the clinical diagnostic value of serum miR-195-5p levels in CVS after SAH (SAH + CVS) and its relationship with the prognosis of SAH + CVS. Methods: A total of 110 patients with spontaneous SAH were divided into the SAH group (N = 62) and SAH + CVS group (N = 58), with 60 healthy subjects as controls. The clinical data of blood glucose, blood sodium fluctuation, and serum lactic acid were recorded. miR-195-5p serum level was detected by RT-qPCR and its diagnostic value on SAH + CVS was analyzed by receiver operating characteristic curve. Serum levels of PDGF/IL-6/ET-1 and their correlation with miR-195-5p were analyzed using RT-qPCR, enzyme-linked immunosorbent assay, and Pearson's method. The patient prognosis was evaluated by Glasgow Outcome Scale. The effect of miR-195-5p levels on adverse prognosis was analyzed by Kaplan-Meier method and Cox regression analysis. Results: miR-195-5p was lowly expressed in the serum of SAH patients and lower in SAH + CVS patients. Serum miR-195-5p level assisted the diagnosis of SAH and SAH+ CVS and was negatively correlated with PDGF/IL-6/ET-1 levels and was an independent risk factor together with ET-1 and blood glucose for SAH + CVS. miR-195-5p low expression predicted a higher cumulative incidence of adverse outcomes and was an independent predictor of adverse outcomes. Conclusion:Poor expression of miR-195-5p can assist the diagnosis of SAH + CVS and predict higher adverse outcomes.

MicroRNAs’ Role in Diagnosis and Treatment of Subarachnoid Hemorrhage

“…MiRNAs play a crucial role in controlling gene expression, including genes involved in SAH, which can lead to both overexpression and under expression of target genes [ 41 ]. Studies have also shown that low expression of miR-195-5p is linked to cerebral vasospasm and poor outcomes in SAH patients [ 75 ]. In other studies, low levels of miR-630 in the CSF were associated with better endothelial function after SAH [ 76 ].…”

MicroRNAs Role in Diagnosis and Treatment of Subarachnoid Hemorrhage

“…MiR-NAs play a crucial role in controlling gene expression; including genes involved in SAH, which can lead to both overexpression and underexpression of target genes 41 . Studies have also shown that low expression of miR-195-5p is linked to cerebral vasospasm and poor outcomes in SAH patients 75 . On the other hand, in other studies low level of miR-630 in the CSF were associated with better endothelial function after SAH 76 .…”