Pomegranate (<i>Punica granatum</i>) peel extract provides protection against mercuric chloride-induced oxidative stress in Wistar strain rats

Kumar, Dinesh; Singh, Sandeep; Singh, Amit Kumar; Rizvi, Syed Ibrahim

doi:10.3109/13880209.2012.738333

Cited by 26 publications

(18 citation statements)

References 26 publications

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“…40 The activity of PMRS has been shown to be elevated in erythrocytes of aged rats 19 and also in rats subjected to oxidative stress in vivo. 41 In view of the important role of erythrocyte PMRS in providing protection against oxidative stress during aging 39 and the hypothesis that the activity of PMRS may be an important determinant of animal life span, 42 the present observation of an increase in PMRS activity in senescent cells signifies increased oxidative stress. The increase in PMRS activity in senescent cells is more pronounced in old (24-month-old) rats, which again substantiates the higher oxidative stress in old rats.…”

Section: Figmentioning

confidence: 91%

Markers of Oxidative Stress in Senescent Erythrocytes Obtained from Young and Old Age Rats

Kumar

Rizvi

2014

Rejuvenation Research

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The role of oxidative stress during aging is well documented. Evidence is available linking animal life span to the development of oxidative stress. Up to a certain limit of oxidative stress, cells function to counteract the oxidant effects and to restore redox balance by resetting critical homeostatic parameters. Red blood cells (RBCs) offer a very good model to study cellular senescence. In vivo aging of red blood cells is associated with increased cellular density, which corresponds to increased cell age. The present study aims to investigate agedependent oxidative stress in RBC subpopulations obtained after Percoll density gradient centrifugation from young and old rats. We observe an increase in plasma membrane redox system (PMRS) activity ( p < 0.001) and lipid peroxidation ( p < 0.001) between less dense and senescent RBCs in both young and old rats. Our findings provide evidence of a higher level of oxidative stress in senescent erythrocytes, with the effect being more pronounced in old (24-month-old) rats compared to young (4-month-old) rats. The present findings emphasize the role of oxidative stress not only in organismal aging but also in cell senescence.

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Section: Figmentioning

confidence: 91%

Markers of Oxidative Stress in Senescent Erythrocytes Obtained from Young and Old Age Rats

Kumar

Rizvi

2014

Rejuvenation Research

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“…Previous studies have demonstrated that heavy metals increase the generation of ROS causing damage to macromolecules, membrane ion transport systems, enzymes, lipid peroxidation in hematological system leading probably to genotoxic effects on blood (Kumar et al 2013;Ashour & El-Shemi 2014) in rats treated by mercury chloride and cadmium, respectively. Barium, an alkaline earth metal, alters hematological parameters including the decrease in blood hemoglobin content and thrombocytes count in industrial workers (Tarasenko et al 1977), while its genotoxicity has not been previously characterised in experimental models.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of pomegranate peel against hematotoxicity, chromosomal aberrations, and genotoxicity induced by barium chloride in adult rats

Elwej

Salah

Kallel

et al. 2016

Pharmaceutical Biology

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Context Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, antiinflammatory, and antimutagenic properties. Objective This study investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl 2 ) in adult rats. Materials and methods Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied. Results PP showed a rich composition of antioxidant compounds. DPPH test found IC 50 value¼ 5.3 mg/mL and a high polysaccharides content (315 ± 5 mg/g of extract). In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats. A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls. BaCl 2 -treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells. Oxidative stress induced by BaCl 2 might be the major cause for chromosomal abnormalities leading to DNA damage. Discussion and conclusion A decrease in hematotoxic and genotoxic effects induced by PP is due to its powerful antioxidant capacity. ARTICLE HISTORY

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“…Exposure to Pb is related to cancer, neurotoxicity, hepatotoxicity, and cardiovascular disorder (Kumar et al, 2013). The method by which NAC and DMSA ameliorated Pb and HgCl 2 toxicity is not examined well till now.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, antioxidants, plant extracts and chelating agents have the potential to inhibit these effects (Kumar et al, 2013;Jarrar, 2003;Mehana et al, 2012;Ekor et al, 2010). Our findings showed that both NAC and DMSA ameliorated the decrease in serum and mRNA expression of antioxidants.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Some Chelating Agents and Antioxidants on the Biohazards Produced From Water Pollution by Heavy Metals in Wistar Rats: Biological, Genetic and Histopathological Study

Baiomy¹,

Soliman²,

Saad³

et al. 2017

Afr J Tradit Complement Altern Med

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Background: Heavy metals that normally cause problems are mercury (HgCl 2 ) and lead acetate (LA). Chelating and inhibitor agents are the target to treat and overcome metal toxicity. The current study has been carried out to evaluate the protective effects of N-acetyl cysteine (NAC) and meso 2,3 dimercaptosuccinic acid (DMSA) against HgCl 2 and LA toxicity. Materials and Methods: Ninety male Wistar rats were divided into nine equal groups. The groups were administered NAC and/or DMSA in presence or absence of LA (LA; 0.2% in drinking water) or HgCl 2 (2 mg/kg BW) for 2 consecutive months. Serum and organs were collected for biochemical, genetic and histopathological changes. Results: Biochemical results revealed that LA and HgCl 2 significantly increased the levels of liver and kidney biomarkers. Administration of NAC and DMSA considerably improved these altered changes. LA and HgCl 2 decreased serum levels of antioxidants and were ameliorated in NAC and DMSA administered rats. LA and HgCl 2 administration upregulated expression of IL-1β and IL-8 that were normalized by NAC and DMSA. Kidneys of LA and HgCl 2 groups showed intraluminal hyaline casts. Kidneys of DMSA-administrated rats showed mild hydropic degeneration of renal tubular epithelium in LA and HgCl 2 groups. Kidneys of NAC administrated rats showed atrophy of capillary tufts. Kidneys of LA and HgCl 2 administered rats which received DMSA and NAC showed normal glomerular structure. Liver histopathology showed sever changes that were ameliorated by NAC and DMSA. Conclusion: Taken together, usage of NAC and DMSA provide significant protection against LA and HgCl 2 -induced hepatotoxicity and nephrotoxicity in male Wistar rats.

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Pomegranate (Punica granatum) peel extract provides protection against mercuric chloride-induced oxidative stress in Wistar strain rats

Cited by 26 publications

References 26 publications

Markers of Oxidative Stress in Senescent Erythrocytes Obtained from Young and Old Age Rats

Markers of Oxidative Stress in Senescent Erythrocytes Obtained from Young and Old Age Rats

Protective effects of pomegranate peel against hematotoxicity, chromosomal aberrations, and genotoxicity induced by barium chloride in adult rats

Protective Effect of Some Chelating Agents and Antioxidants on the Biohazards Produced From Water Pollution by Heavy Metals in Wistar Rats: Biological, Genetic and Histopathological Study

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