Pomegranate Extract Attenuates Gentamicin-Induced Nephrotoxicity in Rats by Reducing Oxidative Stress

Abstract

Nephrotoxicity is a major complication of gentamicin (GEN), which is widely used in the treatment of severe Gramnegative infections. Reactive oxygen species are important mediators of GEN-induced nephrotoxicity. Because of the strong antioxidant properties of pomegranate extract (PE), we evaluated the protective effect of PE against GEN-induced nephrotoxicity. Thirty-two adult male rats were randomly divided into four equal groups: (1) controls; (2) treated with GEN for 14 consecutive days (100 mg/kg/day); (3) treated with GEN plus distilled water; and (4) treated with GEN plus PE (100 μL). After 15 days, the rats were killed and their kidneys were taken, and blood analysis was performed. Tubular necrosis and interstitial fibrosis scores were determined histopathologically; and biochemically, nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels in kidneys were determined. Urea, creatinine, Na þ , and K þ levels were investigated in the blood analysis. Statistical analyses were made by the chi-square test and analysis of variance. Serum urea and creatinine levels were significantly higher in rats treated with GEN alone than rats in the control and the GEN þ PE-treated groups. The GSH level in renal tissue of only GEN-treated rats was significantly lower than those in the control group, and administration of PE to GEN-treated rats significantly increased the level of GSH. The group that was given GEN and PE had significantly lower MDA levels in kidney cortex tissue than those given GEN alone. There was no significant difference of NO levels between the groups. In rats treated with GEN þ PE, despite the presence of mild tubular degeneration and tubular necrosis is less severe, and glomeruli maintained a better morphology when compared with the GEN-treated group. We think that PE prevents kidney damage by decreasing oxidative stress in kidney.

Assessment the Effect of Pomegranate Molasses against Diazinon Toxicity in Male Rats

“…Significant decrease in GSH and an increase in MDA in liver and brain tissue of G2 were partially returned to the control levels by PM in G4 due to the free radical scavenging properties of the PM. These results are in agreement with Cekmen et al [23] who observed that pomegranate extract has strong antioxidant properties against gentamicin induced nephrotoxicity, as it increased the level of GSH and decreased level of MAD in kidney cortex tissue. Mahmoud and Abdel Moneim [24], reported that CCl4 enhanced MAD generation and decreased GSH content in brain tissues of rats, administration of pomegranate juice alone decreased the levels of MAD and increased GSH content of the brain.…”

The protective effect of pomegranate extract against cisplatin toxicity in rat liver and kidney tissue

“…The main phenolic compound in pomegranate flower extract is gallic acid, which is well known for its anti-oxidant activity (Aruoma et al, 1993;Li et al, 2005). Protective effects of aqueous extract of pomegranate have been shown in nephrotoxicity models (Cekmen et al, 2013;Motamedi et al, 2014).…”

The antioxidant effects of pomegranate extract on local and remote organs in a mesenteric ischemia and reperfusion model

“…Pomegranate is suggested to decrease MDA levels. 33 Similarly, when evaluating the preventative effects of pomegranate against lipid peroxidation, we found that MDA levels were significantly lower in the IR + PG group (Group 3) than in the IR group (Group 4) (P = 0.031).…”

“…Similar results in renal tissue have been reported in previous studies. In a recent study from Cekmen et al, 33 pomegranate was reported to be preventive for oxidative stress in kidneys caused by gentamicin treatment. A different study demonstrated that pomegranate had a protective role against carbon tetrachloride-induced toxicity in the kidneys.…”